top of page
mmu_circUnc13b_416
Basic information of circular RNA
PanCircBase ID:
mmu_circUnc13b_416
mm39_Chr. Position ID:
chr4|43091227|43101874|+
Gene name:
Unc13b
Transcript:
ENSMUST00000079978.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
3,4,5,6
Exon/intron offsets:
0,4518,5671,10573
Genomic length:
10647
Exon/intron sizes in nt:
100,118,124,74
Flanking exon/introns:
chr4:43087932-43091227|chr4:43101874-43115102
Splice length:
416
Other database IDs:
CIRCpedia: MMU_CIRCpedia_20008, circAtlas: mmu-Unc13b_0009
circAtlas: hsa-UNC13B_0007, CIRCpedia: HSA_CIRCpedia_63998
Human Ortholog IDs:
Mature spliced Sequence:
ATAAATTTAACACATATGTGACCCTGAAAGTCCAGAACGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAGCAGGATTTCATGTTTGAGATCAGTCGTCTGGACCTGGGCCTAAGTGTGGAGGTGTGGAACAAGGGACTGATATGGGACACAATGGTGGGCACCGTGTGGATTGCACTGAAGACAATCCGTCAGTCGGATGAGGAAGGGCCCGGGGAGTGGTCTACACTGGAGGCAGAGACGTTAATGAAAGATGATGAGATCTGTGGAACTAAAAATCCAACTCCTCATAAAATCTTGCTTGATACAAGATTTGAGCTGCCTTTTGACATCCCAGAGGAGGAAGCCAGATATTGGACCTACAAATTGGAGCAAATCAATGCCTTGGCAGATGATAATGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTGCCTTTTGACATCCCA
59.89
GGAAGGCTGATCACCACGAA
60.04
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTGCCTTTTGACATCCCA
59.89
AGGCTGATCACCACGAACTG
60.04
163
PCR template sequence:
TGATACAAGATTTGAGCTGCCTTTTGACATCCCAGAGGAGGAAGCCAGATATTGGACCTACAAATTGGAGCAAATCAATGCCTTGGCAGATGATAATGAGATAAATTTAACACATATGTGACCCTGAAAGTCCAGAACGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAGCAGGATTTCATGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-130a-3p(1), mmu-miR-148a-3p(1), mmu-miR-326-3p(1), mmu-miR-721(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
AGATGATAATGAGATAAATTTAACAC
siRNA sets
TAAATTTATCTCATTATCA, ATTTATCTCATTATCATCT, AAATTTATCTCATTATCAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUNC13B_416_ORF_1
1.0364
0.256765308
0.545512188
342
ORF sequence:
ATGTTTGAGATCAGTCGTCTGGACCTGGGCCTAAGTGTGGAGGTGTGGAACAAGGGACTGATATGGGACACAATGGTGGGCACCGTGTGGATTGCACTGAAGACAATCCGTCAGTCGGATGAGGAAGGGCCCGGGGAGTGGTCTACACTGGAGGCAGAGACGTTAATGAAAGATGATGAGATCTGTGGAACTAAAAATCCAACTCCTCATAAAATCTTGCTTGATACAAGATTTGAGCTGCCTTTTGACATCCCAGAGGAGGAAGCCAGATATTGGACCTACAAATTGGAGCAAATCAATGCCTTGGCAGATGATAATGAGATAAATTTAACACATATGTGA
Peptide sequence:
MFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEINLTHM
IRESfinder result
IRESfinder score
0.5949226
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page