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mmu_circUggt1_470
Basic information of circular RNA
PanCircBase ID:
mmu_circUggt1_470
mm39_Chr. Position ID:
chr1|36212515|36214183|-
Gene name:
Uggt1
Transcript:
ENSMUST00000046875.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
25,24,23
Exon/intron offsets:
0,644,1461
Genomic length:
1668
Exon/intron sizes in nt:
13,11,32,207
Flanking exon/introns:
chr1:36209375-36212515|chr1:36214183-36215313
Splice length:
470
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
AAAACCAGTAACAATGTTAGGATAAGTATGATCAACAATCCCAGCCAAGAGATCAGTGACTCAAGCACCCCCATCTTCAGAGCCATCTGGGCAGCTCTGCAAACACAGGCCTCCAGCTCTGCTAAGAACTTCATCACCAAGATGGCCAAAGAGGAGACGGCAGAGGCCCTGGCCGCAGGAGTGGACATTGCGGAATTCTCTGTCGGGGGCATGGATGTCAGTCTTTTTAAAGAGGTCTTTGAGTCTTCCAGAATGGATTTCATTTTGTCTCATGCCCTGTACTGCAGGGATGTTCTGAAGCTGAAGAAGGGACAGCGAGTGGTGATCAGCAACGGAAGGATCATTGGGCCTCTGGAGGACAATGAGCTCTTCAACCAAGATGATTTCCACCTCCTGGAAAATATCATCCTGAAAACATCGGGACAGAAAATAAAGTCTCATATCCAACAGCTTCGAGTAGAAGAAGACGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAAGATGATTTCCACCTCCT
59.08
GGGGTGCTTGAGTCACTGAT
59.67
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAAGATGATTTCCACCTCCT
59.08
TGAAGATGGGGGTGCTTGAG
59.67
176
PCR template sequence:
TCAACCAAGATGATTTCCACCTCCTGGAAAATATCATCCTGAAAACATCGGGACAGAAAATAAAGTCTCATATCCAACAGCTTCGAGTAGAAGAAGACGTAAAACCAGTAACAATGTTAGGATAAGTATGATCAACAATCCCAGCCAAGAGATCAGTGACTCAAGCACCCCCATCTTCAGAGCCATCTGGGCAGCTCTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-7b-5p(1), mmu-miR-7a-5p(1), mmu-miR-342-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TAGAAGAAGACGTAAAACCAGTAACA
siRNA sets
TACTGGTTTTACGTCTTCT, TTACTGGTTTTACGTCTTC, TGTTACTGGTTTTACGTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUGGT1_470_ORF_1
1.2288
0.342198252
0.857709467
468
ORF sequence:
ATGATCAACAATCCCAGCCAAGAGATCAGTGACTCAAGCACCCCCATCTTCAGAGCCATCTGGGCAGCTCTGCAAACACAGGCCTCCAGCTCTGCTAAGAACTTCATCACCAAGATGGCCAAAGAGGAGACGGCAGAGGCCCTGGCCGCAGGAGTGGACATTGCGGAATTCTCTGTCGGGGGCATGGATGTCAGTCTTTTTAAAGAGGTCTTTGAGTCTTCCAGAATGGATTTCATTTTGTCTCATGCCCTGTACTGCAGGGATGTTCTGAAGCTGAAGAAGGGACAGCGAGTGGTGATCAGCAACGGAAGGATCATTGGGCCTCTGGAGGACAATGAGCTCTTCAACCAAGATGATTTCCACCTCCTGGAAAATATCATCCTGAAAACATCGGGACAGAAAATAAAGTCTCATATCCAACAGCTTCGAGTAGAAGAAGACGTAAAACCAGTAACAATGTTAGGATAA
Peptide sequence:
MINNPSQEISDSSTPIFRAIWAALQTQASSSAKNFITKMAKEETAEALAAGVDIAEFSVGGMDVSLFKEVFESSRMDFILSHALYCRDVLKLKKGQRVVISNGRIIGPLEDNELFNQDDFHLLENIILKTSGQKIKSHIQQLRVEEDVKPVTMLG
IRESfinder result
IRESfinder score
0.51526821
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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