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mmu_circUggt1_434
Basic information of circular RNA
PanCircBase ID:
mmu_circUggt1_434
mm39_Chr. Position ID:
chr1|36213159|36215408|-
Gene name:
Uggt1
Transcript:
ENSMUST00000046875.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
24,23,22
Exon/intron offsets:
0,817,2154
Genomic length:
2249
Exon/intron sizes in nt:
132,207,95
Flanking exon/introns:
chr1:36212646-36213159|chr1:36215408-36215856
Splice length:
434
Other database IDs:
circBase: hsa_circ_0056422
Human Ortholog IDs:
Mature spliced Sequence:
ATGATTCTTTTATTAGACCAGTGACTTTTTGGATTGTTGGAGATTTTGATAGCCCTTCTGGGCGGCAGCTACTATATGATGCCATTAAACATCAGAAAACCAGTAACAATGTTAGGATAAGTATGATCAACAATCCCAGCCAAGAGATCAGTGACTCAAGCACCCCCATCTTCAGAGCCATCTGGGCAGCTCTGCAAACACAGGCCTCCAGCTCTGCTAAGAACTTCATCACCAAGATGGCCAAAGAGGAGACGGCAGAGGCCCTGGCCGCAGGAGTGGACATTGCGGAATTCTCTGTCGGGGGCATGGATGTCAGTCTTTTTAAAGAGGTCTTTGAGTCTTCCAGAATGGATTTCATTTTGTCTCATGCCCTGTACTGCAGGGATGTTCTGAAGCTGAAGAAGGGACAGCGAGTGGTGATCAGCAACGGAAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTCATGCCCTGTACTGCA
59.75
TATAGTAGCTGCCGCCCAGA
60.18
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCTCATGCCCTGTACTGC
59.75
TATAGTAGCTGCCGCCCAGA
60.18
150
PCR template sequence:
TGAGTCTTCCAGAATGGATTTCATTTTGTCTCATGCCCTGTACTGCAGGGATGTTCTGAAGCTGAAGAAGGGACAGCGAGTGGTGATCAGCAACGGAAGGATGATTCTTTTATTAGACCAGTGACTTTTTGGATTGTTGGAGATTTTGATAGCCCTTCTGGGCGGCAGCTACTATATGATGCCATTAAACATCAGAAAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-7b-5p(1), mmu-miR-7a-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-22-3p(1), mmu-miR-342-5p(1), mmu-miR-224-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGCAACGGAAGGATGATTCTTTTAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUGGT1_434_ORF_1
1.3015
0.325712288
0.713679188
336
ORF sequence:
ATGATCAACAATCCCAGCCAAGAGATCAGTGACTCAAGCACCCCCATCTTCAGAGCCATCTGGGCAGCTCTGCAAACACAGGCCTCCAGCTCTGCTAAGAACTTCATCACCAAGATGGCCAAAGAGGAGACGGCAGAGGCCCTGGCCGCAGGAGTGGACATTGCGGAATTCTCTGTCGGGGGCATGGATGTCAGTCTTTTTAAAGAGGTCTTTGAGTCTTCCAGAATGGATTTCATTTTGTCTCATGCCCTGTACTGCAGGGATGTTCTGAAGCTGAAGAAGGGACAGCGAGTGGTGATCAGCAACGGAAGGATGATTCTTTTATTAGACCAGTGA
Peptide sequence:
MINNPSQEISDSSTPIFRAIWAALQTQASSSAKNFITKMAKEETAEALAAGVDIAEFSVGGMDVSLFKEVFESSRMDFILSHALYCRDVLKLKKGQRVVISNGRMILLLDQ
IRESfinder result
IRESfinder score
0.512871685
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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