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mmu_circTtll7_541
Basic information of circular RNA
PanCircBase ID:
mmu_circTtll7_541
mm39_Chr. Position ID:
chr3|146607078|146615425|+
Gene name:
Ttll7
Transcript:
ENSMUST00000038090.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
6,7,8
Exon/intron offsets:
0,2710,8182
Genomic length:
8347
Exon/intron sizes in nt:
15,92,17,165
Flanking exon/introns:
chr3:146603569-146607078|chr3:146615425-146618984
Splice length:
541
Other database IDs:
circAtlas: mmu-Ttll7_0010
circBase: hsa_circ_0114291, circAtlas: hsa-TTLL7_0082
Human Ortholog IDs:
Mature spliced Sequence:
AATGATCAAGTCTCGGCCTATGGATTATACCTTTGTTCCGAGAACGTGGATTTTCCCTTCTGAATATACTCAGTTCCAAAACTATGTGAAAGAATTGAAGAAAAAACGGAAGCAGAAAACTTTTATAGTAAAACCAGCTAATGGTGCAATGGGTCATGGGATTTCATTGATAAGAAATGGGGATAAAGTCCCTTCTCAGGATCATTTGATAGTTCAAGAATACATTGAGAAACCTTTCCTAATGGAAGGCTACAAGTTTGACTTACGGATTTATATTTTGGTTACATCCTGTGATCCATTAAAGATATTTCTCTACCATGATGGGCTTGTGCGCATGGGCACTGAGAAGTACATCCCACCAAACGAGTCCAACTTGACCCAGTTATACATGCATCTGACCAACTACTCAGTGAACAAACACAATGAACGGTTTGAGCGCAATGAAACTGAAGACAAAGGCAGCAAGCGGTCAATCAAGTGGTTTACGGAATTCCTACAGGCCAATCAACATGATGTCACTAAGTTCTGGAGTGATATTTCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAGACAAAGGCAGCAAGC
59.33
CCACGTTCTCGGAACAAAGG
59.13
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGCAAGCGGTCAATCAAG
60.46
GGGAAAATCCACGTTCTCGG
58.92
140
PCR template sequence:
TGAAACTGAAGACAAAGGCAGCAAGCGGTCAATCAAGTGGTTTACGGAATTCCTACAGGCCAATCAACATGATGTCACTAAGTTCTGGAGTGATATTTCAAATGATCAAGTCTCGGCCTATGGATTATACCTTTGTTCCGAGAACGTGGATTTTCCCTTCTGAATATACTCAGTTCCAAAACTATGTGAAAGAATTGAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-32-5p(1), mmu-miR-25-3p(1), mmu-miR-136-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-539-5p(1), mmu-miR-202-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGTGATATTTCAAATGATCAAGTCT
siRNA sets
CTTGATCATTTGAAATATC, TTGATCATTTGAAATATCA, TGATCATTTGAAATATCAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTTLL7_541_ORF_1
0.7357
0.127945034
0.799889086
660
ORF sequence:
ATGAACGGTTTGAGCGCAATGAAACTGAAGACAAAGGCAGCAAGCGGTCAATCAAGTGGTTTACGGAATTCCTACAGGCCAATCAACATGATGTCACTAAGTTCTGGAGTGATATTTCAAATGATCAAGTCTCGGCCTATGGATTATACCTTTGTTCCGAGAACGTGGATTTTCCCTTCTGAATATACTCAGTTCCAAAACTATGTGAAAGAATTGAAGAAAAAACGGAAGCAGAAAACTTTTATAGTAAAACCAGCTAATGGTGCAATGGGTCATGGGATTTCATTGATAAGAAATGGGGATAAAGTCCCTTCTCAGGATCATTTGATAGTTCAAGAATACATTGAGAAACCTTTCCTAATGGAAGGCTACAAGTTTGACTTACGGATTTATATTTTGGTTACATCCTGTGATCCATTAAAGATATTTCTCTACCATGATGGGCTTGTGCGCATGGGCACTGAGAAGTACATCCCACCAAACGAGTCCAACTTGACCCAGTTATACATGCATCTGACCAACTACTCAGTGAACAAACACAATGAACGGTTTGAGCGCAATGAAACTGAAGACAAAGGCAGCAAGCGGTCAATCAAGTGGTTTACGGAATTCCTACAGGCCAATCAACATGATGTCACTAAGTTCTGGAGTGATATTTCA
Peptide sequence:
MNGLSAMKLKTKAASGQSSGLRNSYRPINMMSLSSGVIFQMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKWFTEFLQANQHDVTKFWSDIS
IRESfinder result
IRESfinder score
0.638611425
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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