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mmu_circTtll5_779
Basic information of circular RNA
PanCircBase ID:
mmu_circTtll5_779
mm39_Chr. Position ID:
chr12|85904153|85938984|+
Gene name:
Ttll5
Transcript:
ENSMUST00000095536.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
6,7,8,9,10,11,12,13,14
Exon/intron offsets:
0,6184,7145,19111,21007,21988,31779,33641,34736
Genomic length:
34831
Exon/intron sizes in nt:
83,70,85,102,92,108,82,62,95
Flanking exon/introns:
chr12:85896155-85904153|chr12:85938984-85940528
Splice length:
779
Other database IDs:
circAtlas: mmu-Ttll5_0043
Human Ortholog IDs:
Mature spliced Sequence:
ATTCATATTCAAAGGACCGAGGACCTTGGATAGTAAAACCAGTGGCCTCTTCTAGGGGGCGGGGCGTCTACTTGATCAACAACCCAAACCAGATCTCCCTGGAAGAGAACATCTTGGTCTCCCGTTACATTAACAACCCCCTGCTCATAGATGATTTCAAGTTTGACGTGCGCCTCTATGTGCTCGTGACTTCCTATGATCCCCTTGTCATCTATCTCTATGAGGAAGGACTGGCTAGGTTTGCAACTGTGCGTTATGATCAAGGCTCCAAGAACATCCGCAACCAGTTCATGCACCTGACGAACTACAGCGTGAACAAGAAGAGTGGAGACTATGTGAGTTGTGATGACCCAGAAGTGGAGGATTATGGGAACAAGTGGAGCATGAGTGCCATGCTTAGGTACCTGAAACAAGAAGGCAAGGACACAACTGCATTGATGGCCCATGTGGAAGACCTGATCATTAAGACAATAATCTCTGCTGAACTAGCTATCGCCACTGCCTGTAAAACCTTTGTTCCACACCGCAGCAGTTGTTTTGAACTGTATGGCTTTGATGTGCTCATAGATAATACTCTGAAGCCATGGTTGTTGGAAGTGAATCTCTCTCCTTCTTTGGCCTGTGATGCTCCTCTGGATCTAAAGATTAAAGCCAGTATGATCTCAGACATGTTCACTGTTGTAGGATTTGTGTGCCAAGATCCTGCCCAGCGGACATCCAACCGGTCAATTTATCCCAGTTTTGAGTCTTCCAGGCGGAACCCTTTTCAGAAACCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGACATCCAACCGGTCAAT
60.39
TTGATCAAGTAGACGCCCCG
59.83
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGTGTGCCAAGATCCTGCC
60.9
TTGATCAAGTAGACGCCCCG
59.83
171
PCR template sequence:
TGTAGGATTTGTGTGCCAAGATCCTGCCCAGCGGACATCCAACCGGTCAATTTATCCCAGTTTTGAGTCTTCCAGGCGGAACCCTTTTCAGAAACCTCAGATTCATATTCAAAGGACCGAGGACCTTGGATAGTAAAACCAGTGGCCTCTTCTAGGGGGCGGGGCGTCTACTTGATCAACAACCCAAACCAGATCTCCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-29b-1-5p(2), mmu-miR-7b-5p(1), mmu-miR-135a-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-7a-5p(1), mmu-miR-185-5p(1), mmu-miR-136-5p(1), mmu-miR-96-5p(1), mmu-miR-34b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGAAACCTCAGATTCATATTCAAA
siRNA sets
TTTGAATATGAATCTGAGG, TATGAATCTGAGGTTTCTG, AATATGAATCTGAGGTTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTTLL5_779_ORF_1
0.9522
0.203017366
0.748041163
522
ORF sequence:
ATGCACCTGACGAACTACAGCGTGAACAAGAAGAGTGGAGACTATGTGAGTTGTGATGACCCAGAAGTGGAGGATTATGGGAACAAGTGGAGCATGAGTGCCATGCTTAGGTACCTGAAACAAGAAGGCAAGGACACAACTGCATTGATGGCCCATGTGGAAGACCTGATCATTAAGACAATAATCTCTGCTGAACTAGCTATCGCCACTGCCTGTAAAACCTTTGTTCCACACCGCAGCAGTTGTTTTGAACTGTATGGCTTTGATGTGCTCATAGATAATACTCTGAAGCCATGGTTGTTGGAAGTGAATCTCTCTCCTTCTTTGGCCTGTGATGCTCCTCTGGATCTAAAGATTAAAGCCAGTATGATCTCAGACATGTTCACTGTTGTAGGATTTGTGTGCCAAGATCCTGCCCAGCGGACATCCAACCGGTCAATTTATCCCAGTTTTGAGTCTTCCAGGCGGAACCCTTTTCAGAAACCTCAGATTCATATTCAAAGGACCGAGGACCTTGGATAG
Peptide sequence:
MHLTNYSVNKKSGDYVSCDDPEVEDYGNKWSMSAMLRYLKQEGKDTTALMAHVEDLIIKTIISAELAIATACKTFVPHRSSCFELYGFDVLIDNTLKPWLLEVNLSPSLACDAPLDLKIKASMISDMFTVVGFVCQDPAQRTSNRSIYPSFESSRRNPFQKPQIHIQRTEDLG
IRESfinder result
IRESfinder score
0.657180328
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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