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mmu_circTenm2_329
Basic information of circular RNA
PanCircBase ID:
mmu_circTenm2_329
mm39_Chr. Position ID:
chr11|36097731|36112357|-
Gene name:
Tenm2
Transcript:
ENSMUST00000102801.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
9,8
Exon/intron offsets:
0,14503
Genomic length:
14626
Exon/intron sizes in nt:
2,06,123
Flanking exon/introns:
chr11:36062348-36097731|chr11:36112357-36164057
Splice length:
329
Other database IDs:
circBase: mmu_circ_0003006, CIRCpedia: MMU_CIRCpedia_18061, circAtlas: mmu-Tenm2_0005
Human Ortholog IDs:
Mature spliced Sequence:
CAATGCATCTGCTCGGACTCAATTGGCAACTCCAGCCGGCAGATGGACACACCTTTAACAATGGCGTAAGGACCGGCTTACCAGGAAACGATGATGTGGCAACAGTGCCATCTGGAGGCAAAGTGCCCTGGTCATTGAAAAACAGCAGCATAGACAGTGGCGAAGCAGAAGTTGGTCGGCGGGTGACACAGGAAGTCCCACCAGGGGTGTTTTGGAGGTCCCAGATTCACATCAGTCAGCCTCAATTCTTAAAGTTCAACATCTCCCTGGGCAAGGATGCCCTCTTCGGTGTCTATATAAGGAGAGGACTACCACCGTCTCATGCCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTTCAACATCTCCCTGGGCA
59.96
TCCTGGTAAGCCGGTCCTTA
59.96
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTTCAACATCTCCCTGGGCA
59.96
ATCGTTTCCTGGTAAGCCGG
60.11
168
PCR template sequence:
CATCAGTCAGCCTCAATTCTTAAAGTTCAACATCTCCCTGGGCAAGGATGCCCTCTTCGGTGTCTATATAAGGAGAGGACTACCACCGTCTCATGCCCAGCAATGCATCTGCTCGGACTCAATTGGCAACTCCAGCCGGCAGATGGACACACCTTTAACAATGGCGTAAGGACCGGCTTACCAGGAAACGATGATGTGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-183-5p(1), mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCTCATGCCCAGCAATGCATCTGCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTENM2_329_ORF_1
0.7502
0.158353759
0.411368514
396
ORF sequence:
ATGCATCTGCTCGGACTCAATTGGCAACTCCAGCCGGCAGATGGACACACCTTTAACAATGGCGTAAGGACCGGCTTACCAGGAAACGATGATGTGGCAACAGTGCCATCTGGAGGCAAAGTGCCCTGGTCATTGAAAAACAGCAGCATAGACAGTGGCGAAGCAGAAGTTGGTCGGCGGGTGACACAGGAAGTCCCACCAGGGGTGTTTTGGAGGTCCCAGATTCACATCAGTCAGCCTCAATTCTTAAAGTTCAACATCTCCCTGGGCAAGGATGCCCTCTTCGGTGTCTATATAAGGAGAGGACTACCACCGTCTCATGCCCAGCAATGCATCTGCTCGGACTCAATTGGCAACTCCAGCCGGCAGATGGACACACCTTTAACAATGGCGTAA
Peptide sequence:
MHLLGLNWQLQPADGHTFNNGVRTGLPGNDDVATVPSGGKVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQQCICSDSIGNSSRQMDTPLTMA
IRESfinder result
IRESfinder score
0.329974824
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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