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mmu_circTbc1d8b_959
Basic information of circular RNA
PanCircBase ID:
mmu_circTbc1d8b_959
mm39_Chr. Position ID:
chrX|138605350|138614168|+
Gene name:
Tbc1d8b
Transcript:
ENSMUST00000096313.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,1354,2515,7652,8653
Genomic length:
8818
Exon/intron sizes in nt:
11,92,26,24,12,08,165
Flanking exon/introns:
chrX:138601858-138605350|chrX:138614168-138624651
Splice length:
959
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAGCCAACAGAGAAGAAATAACAAAACATTGGGACTGGTTGGAACAAAATATAATGAAGACTTTATCTGTGTTTGATTCAAATGAAGATATTACCAATTTTGTACAAGGCAAAATAAGAGGACTAATCGCTGAAGAGGGGAAACAGTCTTTTGCTAAAGAAGATGATCCTGAGAAATTTCGAGAAGCCCTTTTGAAATTTGAAAAATCTTTTGGTTTACCAGAGCAGGAAAAATTAGTAACTTATTATTCCTGCAGTTACTGGAGAGGACGGGTCCCTTGTCAGGGCTGGCTCTATCTAAGTACCAACTTTCTGAGCTTCTATTCCTTCTTGCTAGGATCAGAAATTAAACTCATTATCTCCTGGGATGCAATCTCAAAACTTGAAAAGACTTCAACTGTTATATTAACTGAGAGCATTCACGTATGTTCTCAAGGAGAGAATCACTATTTTTCAATGTTTTTGCACATTAATGAGACATACCTTCTTATGGAACAACTGGCAAACTATGCCATAAAGAGACTTTTTGATAAAGAAACATTTGATAATGACCCAGTCCTTGATGATCCTCTACAGATTACCAAAAGAGGTTTGGAATATCGAGCCCACAGTGAGCAATTCAAAGCCTTTTTTAGGCTACCCAAAGAAGAGACATTGAAAGAAGTACATGAGTGTTTCTTATGGGTACCATTCAGCCACTTCAGTAGTCACGGAAAAATGTGCATTTCAGAAAACTATATTTGCTTTGCTAGCCAGGATGGGAATCTATGCAGTGTAATCATTCCATTACGAGAGGTCTTAGCTATAGATAAAACTGATGACTCCAACAGATCGGTCATCATTAGCATCAAAGGAAAAACAGCTTTTCGTTTCAGTGAGCTTAAAGACTTTGAGCAACTGGTGGCAAAACTCAGGCTCAAGTGCCGGGCAGCTTCAACTCAAGATGATGTTAGTACAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTTTTCGTTTCAGTGAGCT
58.43
TGTTCCAACCAGTCCCAATGT
59.78
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTTTTCGTTTCAGTGAGCTT
59.05
TGTTCCAACCAGTCCCAATGT
59.78
146
PCR template sequence:
AGCTTTTCGTTTCAGTGAGCTTAAAGACTTTGAGCAACTGGTGGCAAAACTCAGGCTCAAGTGCCGGGCAGCTTCAACTCAAGATGATGTTAGTACAGAGGAGCCAACAGAGAAGAAATAACAAAACATTGGGACTGGTTGGAACAAAATATAATGAAGACTTTATCTGTGTTTGATTCAAATGAAGATATTACCAATTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-19b-3p(1), mmu-miR-19a-3p(1), mmu-miR-324-5p(1), mmu-miR-362-5p(1), mmu-miR-301a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTTAGTACAGAGGAGCCAACAGAGA
siRNA sets
TCTCTGTTGGCTCCTCTGT, TTGGCTCCTCTGTACTAAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTBC1D8B_959_ORF_1
0.6837
0.007111999
0.937150184
927
ORF sequence:
ATGAAGACTTTATCTGTGTTTGATTCAAATGAAGATATTACCAATTTTGTACAAGGCAAAATAAGAGGACTAATCGCTGAAGAGGGGAAACAGTCTTTTGCTAAAGAAGATGATCCTGAGAAATTTCGAGAAGCCCTTTTGAAATTTGAAAAATCTTTTGGTTTACCAGAGCAGGAAAAATTAGTAACTTATTATTCCTGCAGTTACTGGAGAGGACGGGTCCCTTGTCAGGGCTGGCTCTATCTAAGTACCAACTTTCTGAGCTTCTATTCCTTCTTGCTAGGATCAGAAATTAAACTCATTATCTCCTGGGATGCAATCTCAAAACTTGAAAAGACTTCAACTGTTATATTAACTGAGAGCATTCACGTATGTTCTCAAGGAGAGAATCACTATTTTTCAATGTTTTTGCACATTAATGAGACATACCTTCTTATGGAACAACTGGCAAACTATGCCATAAAGAGACTTTTTGATAAAGAAACATTTGATAATGACCCAGTCCTTGATGATCCTCTACAGATTACCAAAAGAGGTTTGGAATATCGAGCCCACAGTGAGCAATTCAAAGCCTTTTTTAGGCTACCCAAAGAAGAGACATTGAAAGAAGTACATGAGTGTTTCTTATGGGTACCATTCAGCCACTTCAGTAGTCACGGAAAAATGTGCATTTCAGAAAACTATATTTGCTTTGCTAGCCAGGATGGGAATCTATGCAGTGTAATCATTCCATTACGAGAGGTCTTAGCTATAGATAAAACTGATGACTCCAACAGATCGGTCATCATTAGCATCAAAGGAAAAACAGCTTTTCGTTTCAGTGAGCTTAAAGACTTTGAGCAACTGGTGGCAAAACTCAGGCTCAAGTGCCGGGCAGCTTCAACTCAAGATGATGTTAGTACAGAGGAGCCAACAGAGAAGAAATAA
Peptide sequence:
MKTLSVFDSNEDITNFVQGKIRGLIAEEGKQSFAKEDDPEKFREALLKFEKSFGLPEQEKLVTYYSCSYWRGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDAISKLEKTSTVILTESIHVCSQGENHYFSMFLHINETYLLMEQLANYAIKRLFDKETFDNDPVLDDPLQITKRGLEYRAHSEQFKAFFRLPKEETLKEVHECFLWVPFSHFSSHGKMCISENYICFASQDGNLCSVIIPLREVLAIDKTDDSNRSVIISIKGKTAFRFSELKDFEQLVAKLRLKCRAASTQDDVSTEEPTEKK
IRESfinder result
IRESfinder score
0.774223548
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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