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mmu_circTbc1d32_667
Basic information of circular RNA
PanCircBase ID:
mmu_circTbc1d32_667
mm39_Chr. Position ID:
chr10|56063838|56074656|-
Gene name:
Tbc1d32
Transcript:
ENSMUST00000099739.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
11,10,9,8,7,6,5
Exon/intron offsets:
0,5097,7613,8665,8815,9045,10692
Genomic length:
10818
Exon/intron sizes in nt:
91,60,14,56,21,04,79,100
Flanking exon/introns:
chr10:56056716-56063838|chr10:56074656-56077983
Splice length:
667
Other database IDs:
circAtlas: mmu-Tbc1d32_0014
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGATATGAAGCTTTACAAACATTGTGCTCAGCTCCCCCATCCGACGTCCTGAGCTGTGAGAACTGGACCACTCTCTGTGAAAAGCTGACCACATCTCTGTCTGATCCAGATCCTATGTTCACTGACCGGATTTTAAAGTTCTACGCACAGACTTTTACCCTCTCACCGTTACATATGACCAAGGAGATTTATACAAGCTTAGCTAAATATCTGGAGGTATATTTTCTTTCTAGAGAAAATCACCTTCCTACTCTTTCAACTGGAGTAGACATAACCAGTCCCAATGTGACCCGCTTACTGAAAAAGGTTCGCCTTCTGAATGAGTACCAGAAAGAGGCTCCATCTTTTTGGATTCGTCACCCAGAGAAGTATATGGAGGAAATTGTGGAGAGTACTCTGTCCTTGCTCTCAGTCAAACACGAGCAAAGCCACCTGGTGCCACAGAAGATTTTGGATCCCATCTATTTTTTCGCATTAGTTGATACTAAGGCTGTGTGGTTCAAAAAGTGGATGCATGCATATTACAGCAGAACTGCAGTACTGAGACTTCTTGAAAAGAAATATAAATGTCTGATAACTACAGCAGTTCAGCAATGTGTTCAGTACTTGGAGTTATGTGAAGCTATGAAAGCTGATGAAATTCTGAGACATCCAAAACATTGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGCAGTTCAGCAATGTGT
58.32
GTTCTCACAGCTCAGGACGT
59.68
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGCAGTTCAGCAATGTGT
58.32
GTGGTCCAGTTCTCACAGCT
59.61
159
PCR template sequence:
AAATGTCTGATAACTACAGCAGTTCAGCAATGTGTTCAGTACTTGGAGTTATGTGAAGCTATGAAAGCTGATGAAATTCTGAGACATCCAAAACATTGTGGTGAGATATGAAGCTTTACAAACATTGTGCTCAGCTCCCCCATCCGACGTCCTGAGCTGTGAGAACTGGACCACTCTCTGTGAAAAGCTGACCACATCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-23a-3p(1), mmu-miR-434-3p(1), mmu-miR-383-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCAAAACATTGTGGTGAGATATGAAG
siRNA sets
TTCATATCTCACCACAATG,TCATATCTCACCACAATGT,TCTCACCACAATGTTTTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTBC1D32_667_ORF_1
0.8503
0.014222637
0.601918899
561
ORF sequence:
ATGTTCACTGACCGGATTTTAAAGTTCTACGCACAGACTTTTACCCTCTCACCGTTACATATGACCAAGGAGATTTATACAAGCTTAGCTAAATATCTGGAGGTATATTTTCTTTCTAGAGAAAATCACCTTCCTACTCTTTCAACTGGAGTAGACATAACCAGTCCCAATGTGACCCGCTTACTGAAAAAGGTTCGCCTTCTGAATGAGTACCAGAAAGAGGCTCCATCTTTTTGGATTCGTCACCCAGAGAAGTATATGGAGGAAATTGTGGAGAGTACTCTGTCCTTGCTCTCAGTCAAACACGAGCAAAGCCACCTGGTGCCACAGAAGATTTTGGATCCCATCTATTTTTTCGCATTAGTTGATACTAAGGCTGTGTGGTTCAAAAAGTGGATGCATGCATATTACAGCAGAACTGCAGTACTGAGACTTCTTGAAAAGAAATATAAATGTCTGATAACTACAGCAGTTCAGCAATGTGTTCAGTACTTGGAGTTATGTGAAGCTATGAAAGCTGATGAAATTCTGAGACATCCAAAACATTGTGGTGAGATATGA
Peptide sequence:
MFTDRILKFYAQTFTLSPLHMTKEIYTSLAKYLEVYFLSRENHLPTLSTGVDITSPNVTRLLKKVRLLNEYQKEAPSFWIRHPEKYMEEIVESTLSLLSVKHEQSHLVPQKILDPIYFFALVDTKAVWFKKWMHAYYSRTAVLRLLEKKYKCLITTAVQQCVQYLELCEAMKADEILRHPKHCGEI
IRESfinder result
IRESfinder score
0.784491145
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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