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mmu_circTbc1d14_797
Basic information of circular RNA
PanCircBase ID:
mmu_circTbc1d14_797
mm39_Chr. Position ID:
chr5|36728639|36742180|-
Gene name:
Tbc1d14
Transcript:
ENSMUST00000124036.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,2
Exon/intron offsets:
0,13486
Genomic length:
13541
Exon/intron sizes in nt:
742,55
Flanking exon/introns:
chr5:36700641-36728639|chr5:36742180-36743311
Splice length:
797
Other database IDs:
circBase: mmu_circ_0012607, CIRCpedia: MMU_CIRCpedia_37254, circAtlas: mmu-Tbc1d14_0019
Human Ortholog IDs:
Mature spliced Sequence:
ACGCAAGTTACAATGAGGCTTTATCTAGACCGCTTCTGGGGACTGATGGCAAAAGTTTCTTCTGGGACCAAGATGACTGATGGAAACCTTTCCACCTCGATGAATGGTGTAGCATTGATGGGCATCCTAGATGGTCGGCAAGGAGACTCCCTTCAGGACCTACAACACCTTAGTATCAAGGCGGCTCCCAGATCCCTTTCAGTGCCTGACTACGGACCTTCACTAAAACTTGGTGCTTTGGAAGATCGACACAGCCTTCAATCAGTGGACTCGGGCATTCCTACCCTGGAGATTGGCAACCCAGAGCCTGTTCCCTGCAGTGTGGTCCATGTGAAGAGAAAGCAGTCTGAGTCAGAGATCGTCCCGGAGCGGGCCTTCCAGAGTGCATGCCCGCTGCCGTCGTGCACACCCTCAGCTCCCACCTGCAGCGAGCGGGAGCAGGTTGTGCGGAAGTCTTCCACATTTCCTAGGACAGGCTATGACTCAGTGAAACTCTACAGCCCCACCTCCAAAGCCTTGAGCCGAAGTGACAATGTCTCTGTCTGCAGTGTGTCTAGTCTTGGCACAGAGCTGTCAACTACGTTGTCGGTCAGCAATGAGGACATCTTGGACCTCATGGTCACGAGCAATTCCAGCGCTATTGTGACCCTGGAGAATGATGATGACCCACAGTTTACTGACGTCACCCTGAGCTCCATCAATGAAACCAGCGACTTACACCAGCAGGACTGTGTTGCTGAGACTGAGGAGGGGAGGAAATTGAAACTATTGCACCCATTCAGTCACTTCTTTACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACCAGCGACTTACACCAGC
60.6
TTGCCATCAGTCCCCAGAAG
59.67
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACCAGCGACTTACACCAGC
60.6
ACTTTTGCCATCAGTCCCCA
59.52
149
PCR template sequence:
TCAATGAAACCAGCGACTTACACCAGCAGGACTGTGTTGCTGAGACTGAGGAGGGGAGGAAATTGAAACTATTGCACCCATTCAGTCACTTCTTTACAAGACGCAAGTTACAATGAGGCTTTATCTAGACCGCTTCTGGGGACTGATGGCAAAAGTTTCTTCTGGGACCAAGATGACTGATGGAAACCTTTCCACCTCGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-326-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-370-3p(1), mmu-miR-666-5p(1), mmu-miR-705(1), mmu-miR-29b-2-5p(1), mmu-miR-452-3p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
ACTTCTTTACAAGACGCAAGTTACAA
siRNA sets
TAACTTGCGTCTTGTAAAG, AACTTGCGTCTTGTAAAGA, TTGTAACTTGCGTCTTGTA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTBC1D14_797_ORF_1
0.8953
0.241069942
0.958927116
801
ORF sequence:
ATGAGGCTTTATCTAGACCGCTTCTGGGGACTGATGGCAAAAGTTTCTTCTGGGACCAAGATGACTGATGGAAACCTTTCCACCTCGATGAATGGTGTAGCATTGATGGGCATCCTAGATGGTCGGCAAGGAGACTCCCTTCAGGACCTACAACACCTTAGTATCAAGGCGGCTCCCAGATCCCTTTCAGTGCCTGACTACGGACCTTCACTAAAACTTGGTGCTTTGGAAGATCGACACAGCCTTCAATCAGTGGACTCGGGCATTCCTACCCTGGAGATTGGCAACCCAGAGCCTGTTCCCTGCAGTGTGGTCCATGTGAAGAGAAAGCAGTCTGAGTCAGAGATCGTCCCGGAGCGGGCCTTCCAGAGTGCATGCCCGCTGCCGTCGTGCACACCCTCAGCTCCCACCTGCAGCGAGCGGGAGCAGGTTGTGCGGAAGTCTTCCACATTTCCTAGGACAGGCTATGACTCAGTGAAACTCTACAGCCCCACCTCCAAAGCCTTGAGCCGAAGTGACAATGTCTCTGTCTGCAGTGTGTCTAGTCTTGGCACAGAGCTGTCAACTACGTTGTCGGTCAGCAATGAGGACATCTTGGACCTCATGGTCACGAGCAATTCCAGCGCTATTGTGACCCTGGAGAATGATGATGACCCACAGTTTACTGACGTCACCCTGAGCTCCATCAATGAAACCAGCGACTTACACCAGCAGGACTGTGTTGCTGAGACTGAGGAGGGGAGGAAATTGAAACTATTGCACCCATTCAGTCACTTCTTTACAAGACGCAAGTTACAATGA
Peptide sequence:
MRLYLDRFWGLMAKVSSGTKMTDGNLSTSMNGVALMGILDGRQGDSLQDLQHLSIKAAPRSLSVPDYGPSLKLGALEDRHSLQSVDSGIPTLEIGNPEPVPCSVVHVKRKQSESEIVPERAFQSACPLPSCTPSAPTCSEREQVVRKSSTFPRTGYDSVKLYSPTSKALSRSDNVSVCSVSSLGTELSTTLSVSNEDILDLMVTSNSSAIVTLENDDDPQFTDVTLSSINETSDLHQQDCVAETEEGRKLKLLHPFSHFFTRRKLQ
IRESfinder result
IRESfinder score
0.452664465
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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