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mmu_circTango2_489
Basic information of circular RNA
PanCircBase ID:
mmu_circTango2_489
mm39_Chr. Position ID:
chr16|18125833|18142314|-
Gene name:
Tango2
Transcript:
ENSMUST00000115628.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
6,5,4,3,2
Exon/intron offsets:
0,2899,4669,8676,16387
Genomic length:
16481
Exon/intron sizes in nt:
71,115,120,89,94
Flanking exon/introns:
chr16:18121700-18125833|chr16:18142314-18161758
Splice length:
489
Other database IDs:
circAtlas: mmu-Tango2_0002
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_35956
Human Ortholog IDs:
Mature spliced Sequence:
ACCTTGTGACCGTGTGAGCAAAGCCACCCTTGCAACCCATGTGCATCATCTTCTTTAAGTTTGATCCTCGTCCTGTTTCCAAAAATGCATACAGGCTCATCTTGGCAGCCAACAGAGATGAATTCTATAACAGGCCGTCCAAGTTGGCAGACTTCTGGGGGAACAACAGTGAGATCCTCAGTGGGCTCGACATGGAGGAAGGCAAGGCAGGAGGAACATGGCTGGGCATCAGCACACGTGGGAAGCTGGGGGCACTCACCAACTACCTACAGCCCCGGCAAGAGCCAGACGCCCGTGGTAGAGGTGAACTTGTGTCTCACTTTCTGACCAGTGACATGGACAGCCTGTCCTACCTGAAGAAGGTCTCTACAGAGGGCCACCTGTATAACGGCTTTAACATCATAGCAGCTGACCTGAGCACGTCAAAAGGAGATGTTGTTTGCTACTATGGAAACCGGGGGGAGCCTGAGCCTATTGTCTTGACACCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCATAGCAGCTGACCTGAGC
59.54
GATGATGCACATGGGTTGCA
59.18
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CATAGCAGCTGACCTGAGCA
59.82
AGATGATGCACATGGGTTGC
58.89
140
PCR template sequence:
GGCTTTAACATCATAGCAGCTGACCTGAGCACGTCAAAAGGAGATGTTGTTTGCTACTATGGAAACCGGGGGGAGCCTGAGCCTATTGTCTTGACACCAGACCTTGTGACCGTGTGAGCAAAGCCACCCTTGCAACCCATGTGCATCATCTTCTTTAAGTTTGATCCTCGTCCTGTTTCCAAAAATGCATACAGGCTCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-328-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
GTCTTGACACCAGACCTTGTGACCGT
siRNA sets
ACAAGGTCTGGTGTCAAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTANGO2_489_ORF_1
0.995
0.318745551
0.779853756
468
ORF sequence:
ATGTGCATCATCTTCTTTAAGTTTGATCCTCGTCCTGTTTCCAAAAATGCATACAGGCTCATCTTGGCAGCCAACAGAGATGAATTCTATAACAGGCCGTCCAAGTTGGCAGACTTCTGGGGGAACAACAGTGAGATCCTCAGTGGGCTCGACATGGAGGAAGGCAAGGCAGGAGGAACATGGCTGGGCATCAGCACACGTGGGAAGCTGGGGGCACTCACCAACTACCTACAGCCCCGGCAAGAGCCAGACGCCCGTGGTAGAGGTGAACTTGTGTCTCACTTTCTGACCAGTGACATGGACAGCCTGTCCTACCTGAAGAAGGTCTCTACAGAGGGCCACCTGTATAACGGCTTTAACATCATAGCAGCTGACCTGAGCACGTCAAAAGGAGATGTTGTTTGCTACTATGGAAACCGGGGGGAGCCTGAGCCTATTGTCTTGACACCAGACCTTGTGACCGTGTGA
Peptide sequence:
MCIIFFKFDPRPVSKNAYRLILAANRDEFYNRPSKLADFWGNNSEILSGLDMEEGKAGGTWLGISTRGKLGALTNYLQPRQEPDARGRGELVSHFLTSDMDSLSYLKKVSTEGHLYNGFNIIAADLSTSKGDVVCYYGNRGEPEPIVLTPDLVTV
IRESfinder result
IRESfinder score
0.588042163
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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