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mmu_circTanc2_600
Basic information of circular RNA
PanCircBase ID:
mmu_circTanc2_600
mm39_Chr. Position ID:
chr11|105667631|105689650|+
Gene name:
Tanc2
Transcript:
ENSMUST00000100330.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,6,7
Exon/intron offsets:
0,3133,21755
Genomic length:
22019
Exon/intron sizes in nt:
149,187,264
Flanking exon/introns:
chr11:105604279-105667631|chr11:105689650-105701297
Splice length:
600
Other database IDs:
CIRCpedia: MMU_CIRCpedia_1557, circAtlas: mmu-Tanc2_0005
circBase: hsa_circ_0045172, circAtlas: hsa-TANC2_0009, CIRCpedia: HSA_CIRCpedia_120282
Human Ortholog IDs:
Mature spliced Sequence:
GTGATGTTGAACAAGAACTTGGCCCACCTCCATCTGTAGATGAGGCAGCAAACACACTCATGACTCGTCTGGGTTTCCTCCTTGGAGAGAAAGTCACAGAAGTACAACCAAGTGACCAATATAGCATGGAGGTACAGGATGAAAATCAGACTTCAGCAATCACCCAGAGAATAAGTCCCTGCTCCACCTTGACTAGCAGCACTGCCTCTCCACCAGCCAGCAGCCCCTGCTCTACGCTCCCACCAGTCAGTACAAATGCAGCTGCCAAGGACTGTAGCTATGGGGCTGTCACCAGCCCCACCTCCACTCTAGAAAGCAGAGACAGTGGCATCATTGCAACATTAACAAATTATTCTGAAAACATGGAGCGAACAAAATATGTTGGAGAAGGCAGTAAAGAATTAGGATCTGGAGGAAACCTAAAACCATGGCAGTCTCAAAAATCCAGCATGGACTCTTGTCTGTACCGAGTGGATGAAAACATGGCTGCATCCACATACAGTCTGAACAAGATCCCGGAGCGAAATCTGGAAACAGTTTTATCGCAATCAGTACAGTCTATTCCGTTGTATCTCATGCCTCGGCCAAATTCTGTAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAACAAGATCCCGGAGCGAA
60.11
TGAGTGTGTTTGCTGCCTCA
60.11
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAACAAGATCCCGGAGCGAA
60.11
AGGAGGAAACCCAGACGAGT
60.18
177
PCR template sequence:
AGTCTGAACAAGATCCCGGAGCGAAATCTGGAAACAGTTTTATCGCAATCAGTACAGTCTATTCCGTTGTATCTCATGCCTCGGCCAAATTCTGTAGCAGGTGATGTTGAACAAGAACTTGGCCCACCTCCATCTGTAGATGAGGCAGCAAACACACTCATGACTCGTCTGGGTTTCCTCCTTGGAGAGAAAGTCACAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-335-3p(2), mmu-miR-375-3p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-34b-5p(1), mmu-miR-297a-5p(1), mmu-miR-705(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AATTCTGTAGCAGGTGATGTTGAACA
siRNA sets
TCAACATCACCTGCTACAG, TTCAACATCACCTGCTACA, GTTCAACATCACCTGCTAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTANC2_600_ORF_1
0.7796
0.154506003
0.993548179
1140
ORF sequence:
ATGACTCGTCTGGGTTTCCTCCTTGGAGAGAAAGTCACAGAAGTACAACCAAGTGACCAATATAGCATGGAGGTACAGGATGAAAATCAGACTTCAGCAATCACCCAGAGAATAAGTCCCTGCTCCACCTTGACTAGCAGCACTGCCTCTCCACCAGCCAGCAGCCCCTGCTCTACGCTCCCACCAGTCAGTACAAATGCAGCTGCCAAGGACTGTAGCTATGGGGCTGTCACCAGCCCCACCTCCACTCTAGAAAGCAGAGACAGTGGCATCATTGCAACATTAACAAATTATTCTGAAAACATGGAGCGAACAAAATATGTTGGAGAAGGCAGTAAAGAATTAGGATCTGGAGGAAACCTAAAACCATGGCAGTCTCAAAAATCCAGCATGGACTCTTGTCTGTACCGAGTGGATGAAAACATGGCTGCATCCACATACAGTCTGAACAAGATCCCGGAGCGAAATCTGGAAACAGTTTTATCGCAATCAGTACAGTCTATTCCGTTGTATCTCATGCCTCGGCCAAATTCTGTAGCAGGTGATGTTGAACAAGAACTTGGCCCACCTCCATCTGTAGATGAGGCAGCAAACACACTCATGACTCGTCTGGGTTTCCTCCTTGGAGAGAAAGTCACAGAAGTACAACCAAGTGACCAATATAGCATGGAGGTACAGGATGAAAATCAGACTTCAGCAATCACCCAGAGAATAAGTCCCTGCTCCACCTTGACTAGCAGCACTGCCTCTCCACCAGCCAGCAGCCCCTGCTCTACGCTCCCACCAGTCAGTACAAATGCAGCTGCCAAGGACTGTAGCTATGGGGCTGTCACCAGCCCCACCTCCACTCTAGAAAGCAGAGACAGTGGCATCATTGCAACATTAACAAATTATTCTGAAAACATGGAGCGAACAAAATATGTTGGAGAAGGCAGTAAAGAATTAGGATCTGGAGGAAACCTAAAACCATGGCAGTCTCAAAAATCCAGCATGGACTCTTGTCTGTACCGAGTGGATGAAAACATGGCTGCATCCACATACAGTCTGAACAAGATCCCGGAGCGAAATCTGGAAACAGTTTTATCGCAATCAGTACAGTCTATTCCGTTGTATCTCATGCCTCGGCCAAATTCTGTAGCA
Peptide sequence:
MTRLGFLLGEKVTEVQPSDQYSMEVQDENQTSAITQRISPCSTLTSSTASPPASSPCSTLPPVSTNAAAKDCSYGAVTSPTSTLESRDSGIIATLTNYSENMERTKYVGEGSKELGSGGNLKPWQSQKSSMDSCLYRVDENMAASTYSLNKIPERNLETVLSQSVQSIPLYLMPRPNSVAGDVEQELGPPPSVDEAANTLMTRLGFLLGEKVTEVQPSDQYSMEVQDENQTSAITQRISPCSTLTSSTASPPASSPCSTLPPVSTNAAAKDCSYGAVTSPTSTLESRDSGIIATLTNYSENMERTKYVGEGSKELGSGGNLKPWQSQKSSMDSCLYRVDENMAASTYSLNKIPERNLETVLSQSVQSIPLYLMPRPNSVA
IRESfinder result
IRESfinder score
0.533991103
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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