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mmu_circTagln2_278
Basic information of circular RNA
PanCircBase ID:
mmu_circTagln2_278
mm39_Chr. Position ID:
chr1|172333327|172333855|+
Gene name:
Tagln2
Transcript:
ENSMUST00000111230.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,425
Genomic length:
528
Exon/intron sizes in nt:
1,75,103
Flanking exon/introns:
chr1:172332894-172333327|chr1:172333855-172334133
Splice length:
278
Other database IDs:
CIRCpedia: HSA_CIRCpedia_351331
Human Ortholog IDs:
Mature spliced Sequence:
GTTCTGTGCAAGCTTATTAATTCACTGTATCCTGAGGGGCAGGCCCCAGTAAAGAAGATCCAGGCCTCTTCGATGGCCTTCAAGCAGATGGAGCAGATCTCCCAGTTCCTGCAGGCAGCCGAGCGCTATGGCATTAACACCACGGACATCTTCCAGACTGTGGATCTCTGGGAAGGAAAGAACATGGCTTGTGTGCAGCGGACACTAATGAACCTGGGTGGGCTGGCAGTAGCCAGGGACGATGGGCTCTTCTCTGGGGATCCCAACTGGTTTCCTAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGTGCAGCGGACACTAAT
59.97
CCATCGAAGAGGCCTGGATC
59.97
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGTGTGCAGCGGACACTAA
60.18
CCATCGAAGAGGCCTGGATC
59.97
166
PCR template sequence:
AGAACATGGCTTGTGTGCAGCGGACACTAATGAACCTGGGTGGGCTGGCAGTAGCCAGGGACGATGGGCTCTTCTCTGGGGATCCCAACTGGTTTCCTAAGTTCTGTGCAAGCTTATTAATTCACTGTATCCTGAGGGGCAGGCCCCAGTAAAGAAGATCCAGGCCTCTTCGATGGCCTTCAAGCAGATGGAGCAGATCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTGGTTTCCTAAGTTCTGTGCAAGC
siRNA sets
ACAGAACTTAGGAAACCAG, GCACAGAACTTAGGAAACC, CACAGAACTTAGGAAACCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTAGLN2_278_ORF_1
1.0866
0.29084161
0.454008083
258
ORF sequence:
ATGGCCTTCAAGCAGATGGAGCAGATCTCCCAGTTCCTGCAGGCAGCCGAGCGCTATGGCATTAACACCACGGACATCTTCCAGACTGTGGATCTCTGGGAAGGAAAGAACATGGCTTGTGTGCAGCGGACACTAATGAACCTGGGTGGGCTGGCAGTAGCCAGGGACGATGGGCTCTTCTCTGGGGATCCCAACTGGTTTCCTAAGTTCTGTGCAAGCTTATTAATTCACTGTATCCTGAGGGGCAGGCCCCAGTAA
Peptide sequence:
MAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKFCASLLIHCILRGRPQ
IRESfinder result
IRESfinder score
0.411673761
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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