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mmu_circSpata7_182
Basic information of circular RNA
PanCircBase ID:
mmu_circSpata7_182
mm39_Chr. Position ID:
chr12|98603824|98614741|+
Gene name:
Spata7
Transcript:
ENSMUST00000048402.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,5
Exon/intron offsets:
0,10783
Genomic length:
10917
Exon/intron sizes in nt:
48,134
Flanking exon/introns:
chr12:98600578-98603824|chr12:98614741-98624471
Splice length:
182
Other database IDs:
circAtlas: mmu-Spata7_0005
Human Ortholog IDs:
Mature spliced Sequence:
CCGCAGTGGACTGCTCAATTCCAGTAAGTGTGAATACTAGCATCAAATATGCTGACCAACAACGAAGAGAAAAACTGAGGAAGGAGTTGGCACGGTGTGAAAAGGAGTTTAAATTAAGTAAATCTGCTATGCAGACCAATTCTAAGATGAATTCCAAGTTCTTTGTAAATTCTCTACAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACGGTGTGAAAAGGAGTTTAA
55.66
TCTCTTCGTTGTTGGTCAGCA
59.86
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTATGCAGACCAATTCTAAGA
57.64
TCTCTTCGTTGTTGGTCAGCA
59.86
128
PCR template sequence:
ACGGTGTGAAAAGGAGTTTAAATTAAGTAAATCTGCTATGCAGACCAATTCTAAGATGAATTCCAAGTTCTTTGTAAATTCTCTACAGAAGCCGCAGTGGACTGCTCAATTCCAGTAAGTGTGAATACTAGCATCAAATATGCTGACCAACAACGAAGAGAAAAACTGAGGAAGGAGTTGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
TTCTCTACAGAAGCCGCAGTGGACTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSPATA7_182_ORF_1
0.8625
-0.006433232
0.043543661
81
ORF sequence:
ATGCAGACCAATTCTAAGATGAATTCCAAGTTCTTTGTAAATTCTCTACAGAAGCCGCAGTGGACTGCTCAATTCCAGTAA
Peptide sequence:
MQTNSKMNSKFFVNSLQKPQWTAQFQ
IRESfinder result
IRESfinder score
0.617736128
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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