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mmu_circRictor_478
Basic information of circular RNA
PanCircBase ID:
mmu_circRictor_478
mm39_Chr. Position ID:
chr15|6794532|6799684|+
Gene name:
Rictor
Transcript:
ENSMUST00000228266.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
10,11,12,13,14,15
Exon/intron offsets:
0,3002,4194,4693,4876,5071
Genomic length:
5152
Exon/intron sizes in nt:
68,83,88,91,67,81
Flanking exon/introns:
chr15:6793828-6794532|chr15:6799684-6801612
Splice length:
478
Other database IDs:
circAtlas: mmu-Rictor_0024
Human Ortholog IDs:
Mature spliced Sequence:
AGAAGACAGAGAAGCACGATTTCTAGCCAGTAAGATGGGAATCATTGCGACATTCCGCTCGTGGGCAGGTATTATTAATTTATGTAAGCCTGGAAATTCTGGGATACAGTCTCTAATTGGAGTACTTTGCATACCGAATATGGAAATAAGGCGAGGTCTGCTCGAAGTGCTCTATGACATCTTTCGTCTCCCTCTGCCCGTTGTGACGGATGAGTTCATAGAAGCCCTGCTCAGTGTAGATCCAGGAAGATTCCAGGACAGTTGGAGGCTTTCCGATGGCTTTGTGGCAGCTGAGGCAAAAACTATTCTTCCCCATCGTGCTAGATCCAGGCCAGACCTCATGGACAATTATTTGGCTCTGATACTCTCTGCATTTATTCGTAATGGACTTTTAGAGGGCTTGGTCGAGGTGATAACGAACAGTGACGACCACATCTCAGTGAGGGCGACCATCCTTTTAGGAGAGCTCCTGCATATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTAGAGGGCTTGGTCGAGGT
60.25
ATAATACCTGCCCACGAGCG
59.97
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGCTTGGTCGAGGTGATA
60.33
ATAATACCTGCCCACGAGCG
59.97
158
PCR template sequence:
TCGTAATGGACTTTTAGAGGGCTTGGTCGAGGTGATAACGAACAGTGACGACCACATCTCAGTGAGGGCGACCATCCTTTTAGGAGAGCTCCTGCATATGAGAAGACAGAGAAGCACGATTTCTAGCCAGTAAGATGGGAATCATTGCGACATTCCGCTCGTGGGCAGGTATTATTAATTTATGTAAGCCTGGAAATTCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
GCTCCTGCATATGAGAAGACAGAGAA
siRNA sets
TCTCTGTCTTCTCATATGC, CTCTGTCTTCTCATATGCA, TTCTCTGTCTTCTCATATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCRICTOR_478_ORF_1
0.8466
0.024789656
0.463179124
477
ORF sequence:
ATGGGAATCATTGCGACATTCCGCTCGTGGGCAGGTATTATTAATTTATGTAAGCCTGGAAATTCTGGGATACAGTCTCTAATTGGAGTACTTTGCATACCGAATATGGAAATAAGGCGAGGTCTGCTCGAAGTGCTCTATGACATCTTTCGTCTCCCTCTGCCCGTTGTGACGGATGAGTTCATAGAAGCCCTGCTCAGTGTAGATCCAGGAAGATTCCAGGACAGTTGGAGGCTTTCCGATGGCTTTGTGGCAGCTGAGGCAAAAACTATTCTTCCCCATCGTGCTAGATCCAGGCCAGACCTCATGGACAATTATTTGGCTCTGATACTCTCTGCATTTATTCGTAATGGACTTTTAGAGGGCTTGGTCGAGGTGATAACGAACAGTGACGACCACATCTCAGTGAGGGCGACCATCCTTTTAGGAGAGCTCCTGCATATGAGAAGACAGAGAAGCACGATTTCTAGCCAGTAA
Peptide sequence:
MGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTDEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMRRQRSTISSQ
IRESfinder result
IRESfinder score
0.626774485
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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