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mmu_circPpp1r12b_1425
Basic information of circular RNA
PanCircBase ID:
mmu_circPpp1r12b_1425
mm39_Chr. Position ID:
chr1|134793515|134824199|-
Gene name:
Ppp1r12b
Transcript:
ENSMUST00000045665.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,7029,8161,10187,13855,20645,21497,24978,26380,27572,30565
Genomic length:
30684
Exon/intron sizes in nt:
183,126,83,201,113,140,80,75,145,160,119
Flanking exon/introns:
chr1:134770471-134793515|chr1:134824199-134830115
Splice length:
1425
Other database IDs:
circAtlas: mmu-Ppp1r12b_0009
circBase: hsa_circ_0015985, circAtlas: hsa-PPP1R12B_0005, CIRCpedia: HSA_CIRCpedia_29509
Human Ortholog IDs:
Mature spliced Sequence:
GTATTTTATTAGCCATGGAGCCAGTGTGGGTATTGTGAACAGTGAAGGTGAAGTTCCTTCTGACCTTGCAGAGGAGCCAGCCATGAAAGACCTTCTTCTGGAACAAGTAAAGAAACAAGGCGTTGATCTAGAGCAGTCAAGGAAGGAGGAAGAACAGCAGATGTTGCAGGATGCCCGCCAGTGGCTCAACAGCGGAAGAATAGAAGATGTGAGGCAGGCTCGCTCAGGGGCCACTGCCCTGCATGTAGCTGCTGCCAAGGGTTACTCTGAAGTCCTCAGGCTTCTCATTCAGGCTGGCTATGAACTCAATGTTCAGGATCATGACGGCTGGACTCCTCTTCATGCAGCTGCACACTGGGGAGTAAAAGAGGCTTGCTCCATCCTGGCAGAAGCACTCTGTGACATGGATATCCGGAACAAACTGGGCCAGACGCCATTTGATGTGGCTGATGAAGGTCTCGTGGAGCACTTGGAGATGCTTCAGAAGAAGCAAGATGTGCTTCGAAGTGAAAAGGAGACACGGAATAAGCTCATCGAGTCAGACCTGAATAGCAAGTTTCAGAGTGGACTCTTTAAGAACAAAGAGAAGATGCTTTATGAAGAAGAGATCCCGAAGTCCCAAGACACAGAGGAAGAAAACAAAGAGTCCAGCAGCTCCAGCTCAGAGGAGGAGGAAGGGGAAGATGAAGTTTCTGAATCAGAGACTGAGAAGGAGGCAGATAAAAAGCCAGAAGCCACTGTCAACCATTCCAACTCTGAAATCAAGAGTCGGATCATGGAGCAGATCCCAGCCCCAGCTCAGAACACCTTCTCTGCCTCTTCAGCTAGGAGACTGTCCAGTCTTTTCAACAAGGCAGAAGAGCCTAAAGATGAATCTCCCTCTTCCTGGAGACTGGGGCTCAGAAAAACTGGTAGCCACAATATGCTGAGTGAAGTGGCCAATTCCAGGGAGGCCCTTAGGGATCGAGGTTCTTCCATCTACCGGTCCTCCTCAAGCCCTCGGATCTCTGCTTTGCTGGATGACAAAGATAAGGAAAGAGAAAACAAAAGCTATTTCAGTATGCTAGTACCCCGGAGGCTCAGCAGCACAAGTGACATTGAAGAAAAGGAGAACAGAGAGTCAGCTGTTAATCTAGTAAGGAGTGGCTCCCATACCAGGCAGCTGTGGAGGGATGAAGCAAAGGGAAGTGAAACCCCACAGACAATCGCTCCTTCCACTTACACGTCAACTTACTTGAAAAGGACTCCTTACAAGTCCCAGGCTGACTCAACAGCAGAGAAAACAGCAGACAGTGTCTCCTCCAGCACCCCACTGTGTGTGATCACCAACCGTCCTGCGCCAAGTACTGCCAATGGGGTTCCAGCTGCCACAGTGTTCTCCTCCGCTGGAACAGACCCCTCTGTGGAGGCCCGGGAGAAGAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAAGTACTGCCAATGGGGT
59.96
GTCTTTCATGGCTGGCTCCT
60.03
177
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAAGTACTGCCAATGGGGT
59.96
AGGTCTTTCATGGCTGGCTC
60.03
179
PCR template sequence:
CCAACCGTCCTGCGCCAAGTACTGCCAATGGGGTTCCAGCTGCCACAGTGTTCTCCTCCGCTGGAACAGACCCCTCTGTGGAGGCCCGGGAGAAGAGGAGGTATTTTATTAGCCATGGAGCCAGTGTGGGTATTGTGAACAGTGAAGGTGAAGTTCCTTCTGACCTTGCAGAGGAGCCAGCCATGAAAGACCTTCTTCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(2), mmu-miR-298-5p(2), mmu-miR-149-5p(2), mmu-miR-207(2), mmu-miR-452-3p(2), mmu-miR-200a-3p(1), mmu-miR-199a-5p(1), mmu-miR-34a-5p(1), mmu-miR-204-5p(1), mmu-miR-222-3p(1), mmu-miR-709(1), mmu-miR-125b-5p(1), mmu-miR-351-5p(1), mmu-miR-26b-5p(1), mmu-miR-1199-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGGAGAAGAGGAGGTATTTTATTAGC
siRNA sets
AAAATACCTCCTCTTCTCC, TAAAATACCTCCTCTTCTC, AAATACCTCCTCTTCTCCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCPPP1R12B_1425_ORF_1
1.1164
0.314085457
0.999999985
2766
ORF sequence:
ATGAAAGACCTTCTTCTGGAACAAGTAAAGAAACAAGGCGTTGATCTAGAGCAGTCAAGGAAGGAGGAAGAACAGCAGATGTTGCAGGATGCCCGCCAGTGGCTCAACAGCGGAAGAATAGAAGATGTGAGGCAGGCTCGCTCAGGGGCCACTGCCCTGCATGTAGCTGCTGCCAAGGGTTACTCTGAAGTCCTCAGGCTTCTCATTCAGGCTGGCTATGAACTCAATGTTCAGGATCATGACGGCTGGACTCCTCTTCATGCAGCTGCACACTGGGGAGTAAAAGAGGCTTGCTCCATCCTGGCAGAAGCACTCTGTGACATGGATATCCGGAACAAACTGGGCCAGACGCCATTTGATGTGGCTGATGAAGGTCTCGTGGAGCACTTGGAGATGCTTCAGAAGAAGCAAGATGTGCTTCGAAGTGAAAAGGAGACACGGAATAAGCTCATCGAGTCAGACCTGAATAGCAAGTTTCAGAGTGGACTCTTTAAGAACAAAGAGAAGATGCTTTATGAAGAAGAGATCCCGAAGTCCCAAGACACAGAGGAAGAAAACAAAGAGTCCAGCAGCTCCAGCTCAGAGGAGGAGGAAGGGGAAGATGAAGTTTCTGAATCAGAGACTGAGAAGGAGGCAGATAAAAAGCCAGAAGCCACTGTCAACCATTCCAACTCTGAAATCAAGAGTCGGATCATGGAGCAGATCCCAGCCCCAGCTCAGAACACCTTCTCTGCCTCTTCAGCTAGGAGACTGTCCAGTCTTTTCAACAAGGCAGAAGAGCCTAAAGATGAATCTCCCTCTTCCTGGAGACTGGGGCTCAGAAAAACTGGTAGCCACAATATGCTGAGTGAAGTGGCCAATTCCAGGGAGGCCCTTAGGGATCGAGGTTCTTCCATCTACCGGTCCTCCTCAAGCCCTCGGATCTCTGCTTTGCTGGATGACAAAGATAAGGAAAGAGAAAACAAAAGCTATTTCAGTATGCTAGTACCCCGGAGGCTCAGCAGCACAAGTGACATTGAAGAAAAGGAGAACAGAGAGTCAGCTGTTAATCTAGTAAGGAGTGGCTCCCATACCAGGCAGCTGTGGAGGGATGAAGCAAAGGGAAGTGAAACCCCACAGACAATCGCTCCTTCCACTTACACGTCAACTTACTTGAAAAGGACTCCTTACAAGTCCCAGGCTGACTCAACAGCAGAGAAAACAGCAGACAGTGTCTCCTCCAGCACCCCACTGTGTGTGATCACCAACCGTCCTGCGCCAAGTACTGCCAATGGGGTTCCAGCTGCCACAGTGTTCTCCTCCGCTGGAACAGACCCCTCTGTGGAGGCCCGGGAGAAGAGGAGGTATTTTATTAGCCATGGAGCCAGTGTGGGTATTGTGAACAGTGAAGGTGAAGTTCCTTCTGACCTTGCAGAGGAGCCAGCCATGAAAGACCTTCTTCTGGAACAAGTAAAGAAACAAGGCGTTGATCTAGAGCAGTCAAGGAAGGAGGAAGAACAGCAGATGTTGCAGGATGCCCGCCAGTGGCTCAACAGCGGAAGAATAGAAGATGTGAGGCAGGCTCGCTCAGGGGCCACTGCCCTGCATGTAGCTGCTGCCAAGGGTTACTCTGAAGTCCTCAGGCTTCTCATTCAGGCTGGCTATGAACTCAATGTTCAGGATCATGACGGCTGGACTCCTCTTCATGCAGCTGCACACTGGGGAGTAAAAGAGGCTTGCTCCATCCTGGCAGAAGCACTCTGTGACATGGATATCCGGAACAAACTGGGCCAGACGCCATTTGATGTGGCTGATGAAGGTCTCGTGGAGCACTTGGAGATGCTTCAGAAGAAGCAAGATGTGCTTCGAAGTGAAAAGGAGACACGGAATAAGCTCATCGAGTCAGACCTGAATAGCAAGTTTCAGAGTGGACTCTTTAAGAACAAAGAGAAGATGCTTTATGAAGAAGAGATCCCGAAGTCCCAAGACACAGAGGAAGAAAACAAAGAGTCCAGCAGCTCCAGCTCAGAGGAGGAGGAAGGGGAAGATGAAGTTTCTGAATCAGAGACTGAGAAGGAGGCAGATAAAAAGCCAGAAGCCACTGTCAACCATTCCAACTCTGAAATCAAGAGTCGGATCATGGAGCAGATCCCAGCCCCAGCTCAGAACACCTTCTCTGCCTCTTCAGCTAGGAGACTGTCCAGTCTTTTCAACAAGGCAGAAGAGCCTAAAGATGAATCTCCCTCTTCCTGGAGACTGGGGCTCAGAAAAACTGGTAGCCACAATATGCTGAGTGAAGTGGCCAATTCCAGGGAGGCCCTTAGGGATCGAGGTTCTTCCATCTACCGGTCCTCCTCAAGCCCTCGGATCTCTGCTTTGCTGGATGACAAAGATAAGGAAAGAGAAAACAAAAGCTATTTCAGTATGCTAGTACCCCGGAGGCTCAGCAGCACAAGTGACATTGAAGAAAAGGAGAACAGAGAGTCAGCTGTTAATCTAGTAAGGAGTGGCTCCCATACCAGGCAGCTGTGGAGGGATGAAGCAAAGGGAAGTGAAACCCCACAGACAATCGCTCCTTCCACTTACACGTCAACTTACTTGAAAAGGACTCCTTACAAGTCCCAGGCTGACTCAACAGCAGAGAAAACAGCAGACAGTGTCTCCTCCAGCACCCCACTGTGTGTGATCACCAACCGTCCTGCGCCAAGTACTGCCAATGGGGTTCCAGCTGCCACAGTGTTCTCCTCCGCTGGAACAGACCCCTCTGTGGAGGCCCGGGAGAAGAGG
Peptide sequence:
MKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGRIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDHDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLEMLQKKQDVLRSEKETRNKLIESDLNSKFQSGLFKNKEKMLYEEEIPKSQDTEEENKESSSSSSEEEEGEDEVSESETEKEADKKPEATVNHSNSEIKSRIMEQIPAPAQNTFSASSARRLSSLFNKAEEPKDESPSSWRLGLRKTGSHNMLSEVANSREALRDRGSSIYRSSSSPRISALLDDKDKERENKSYFSMLVPRRLSSTSDIEEKENRESAVNLVRSGSHTRQLWRDEAKGSETPQTIAPSTYTSTYLKRTPYKSQADSTAEKTADSVSSSTPLCVITNRPAPSTANGVPAATVFSSAGTDPSVEAREKRRYFISHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGRIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDHDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLEMLQKKQDVLRSEKETRNKLIESDLNSKFQSGLFKNKEKMLYEEEIPKSQDTEEENKESSSSSSEEEEGEDEVSESETEKEADKKPEATVNHSNSEIKSRIMEQIPAPAQNTFSASSARRLSSLFNKAEEPKDESPSSWRLGLRKTGSHNMLSEVANSREALRDRGSSIYRSSSSPRISALLDDKDKERENKSYFSMLVPRRLSSTSDIEEKENRESAVNLVRSGSHTRQLWRDEAKGSETPQTIAPSTYTSTYLKRTPYKSQADSTAEKTADSVSSSTPLCVITNRPAPSTANGVPAATVFSSAGTDPSVEAREKR
IRESfinder result
IRESfinder score
0.480606628
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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