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mmu_circNgly1_1009
Basic information of circular RNA
PanCircBase ID:
mmu_circNgly1_1009
mm39_Chr. Position ID:
chr14|6187877|6215121|-
Gene name:
Ngly1
Transcript:
ENSMUST00000022310.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,6,5,4,3,2
Exon/intron offsets:
0,1071,3864,11245,21085,27129
Genomic length:
27244
Exon/intron sizes in nt:
1,46,12,22,23,16,62,37,000
Flanking exon/introns:
chr14:6186400-6187877|chr14:6215121-6220265
Splice length:
1009
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAACCCCAGTGATGAAAAATACAGATCCATCCGTATTGGGAACACAGCGTTTTCTACTAGACTCTTGCCTGTCAGAGGAGCTGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACGCATCTTATCTTTCCTAAAAAAGCTTCAGTGGAACAGCTACAGAAAATCCGTGATCTGATTGCCATAGAGAGAAGCAGCAGACTGGACGGATCCAGTAAGAAGGTGCAATTCTCTCAGCACCCGGCTGCTGCCAAGCTTCCTCTGGAGCAGTCTGAAGATCCTGCTGGGTTAATACGGCACTCAGGAAACCAAACAGGGCAGCTGCCAAGCCTACCCTCTGCTCCAATGGTTGTTGGTGATTCAACCATCTTAAAAGTTCTTCAGTCCAACATTCAGCATGTGCAGCTCTATGAAAACCCTGTCCTCCAGGAGAAAGCATTGACTTGTATCCCAGTCAGTGAACTAAAAAGAAAAGCTCAAGAAAAGTTATTCAGAGCTAGAAAGTTAGATAAAGGTACTAATGTAAGTGATGAGGACTTCCTTCTACTGGAGCTCCTACACTGGTTTAAAGAGGAGTTTTTTCGCTGGGTGAATAACATTGTGTGCAGCAAATGTGGTGGAGAGACTAGATCTAGAGATGAGGCATTGCTGCCCAATGATGATGAACTGAAGTGGGGTGCAAAGAATGTGGAGAATCATTACTGTGATGCCTGCCAGTTGAGCAACCGCTTCCCAAGATATAACAACCCCGAGAAACTTTTGGAAACAAGATGTGGACGCTGTGGTGAATGGGCCAATTGTTTTACTCTGTGCTGCCGTGCATTAGGGTTTGAAGCTCGATATGTGTGGGATTACACAGACCATGTTTGGACAGAAGTCTATTCTCCATCTCAGCAGCGGTGGCTACACTGTGATGCATGTGAGGATGTCTGTGACAAACCCCTCCTTTATGAAATAGGATGGGGCAAGAAGCTTTCCTATATAATAGCATTCTCTAAAGATGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCTGTGACAAACCCCTCC
59.53
GCTCCTCTGACAGGCAAGAG
60.11
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCTGTGACAAACCCCTCC
59.53
CAGCTCCTCTGACAGGCAAG
60.39
162
PCR template sequence:
ACACTGTGATGCATGTGAGGATGTCTGTGACAAACCCCTCCTTTATGAAATAGGATGGGGCAAGAAGCTTTCCTATATAATAGCATTCTCTAAAGATGAGAAACCCCAGTGATGAAAAATACAGATCCATCCGTATTGGGAACACAGCGTTTTCTACTAGACTCTTGCCTGTCAGAGGAGCTGTTGAGTGTTTATTTGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-32-5p(1), mmu-miR-31-5p(1), mmu-miR-25-3p(1), mmu-miR-381-3p(1), mmu-miR-128-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-5101(1), mmu-miR-335-3p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCTAAAGATGAGAAACCCCAGTGAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNGLY1_1009_ORF_1
0.7527
0.07561433
0.976705636
1014
ORF sequence:
ATGAGAAACCCCAGTGATGAAAAATACAGATCCATCCGTATTGGGAACACAGCGTTTTCTACTAGACTCTTGCCTGTCAGAGGAGCTGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACGCATCTTATCTTTCCTAAAAAAGCTTCAGTGGAACAGCTACAGAAAATCCGTGATCTGATTGCCATAGAGAGAAGCAGCAGACTGGACGGATCCAGTAAGAAGGTGCAATTCTCTCAGCACCCGGCTGCTGCCAAGCTTCCTCTGGAGCAGTCTGAAGATCCTGCTGGGTTAATACGGCACTCAGGAAACCAAACAGGGCAGCTGCCAAGCCTACCCTCTGCTCCAATGGTTGTTGGTGATTCAACCATCTTAAAAGTTCTTCAGTCCAACATTCAGCATGTGCAGCTCTATGAAAACCCTGTCCTCCAGGAGAAAGCATTGACTTGTATCCCAGTCAGTGAACTAAAAAGAAAAGCTCAAGAAAAGTTATTCAGAGCTAGAAAGTTAGATAAAGGTACTAATGTAAGTGATGAGGACTTCCTTCTACTGGAGCTCCTACACTGGTTTAAAGAGGAGTTTTTTCGCTGGGTGAATAACATTGTGTGCAGCAAATGTGGTGGAGAGACTAGATCTAGAGATGAGGCATTGCTGCCCAATGATGATGAACTGAAGTGGGGTGCAAAGAATGTGGAGAATCATTACTGTGATGCCTGCCAGTTGAGCAACCGCTTCCCAAGATATAACAACCCCGAGAAACTTTTGGAAACAAGATGTGGACGCTGTGGTGAATGGGCCAATTGTTTTACTCTGTGCTGCCGTGCATTAGGGTTTGAAGCTCGATATGTGTGGGATTACACAGACCATGTTTGGACAGAAGTCTATTCTCCATCTCAGCAGCGGTGGCTACACTGTGATGCATGTGAGGATGTCTGTGACAAACCCCTCCTTTATGAAATAGGATGGGGCAAGAAGCTTTCCTATATAATAGCATTCTCTAAAGATGAG
Peptide sequence:
MRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDE
IRESfinder result
IRESfinder score
0.596374012
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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