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mmu_circNcoa5_858
Basic information of circular RNA
PanCircBase ID:
mmu_circNcoa5_858
mm39_Chr. Position ID:
chr2|164846016|164865132|-
Gene name:
Ncoa5
Transcript:
ENSMUST00000040381.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
6,5,4,3,2
Exon/intron offsets:
0,5226,6354,8739,19049
Genomic length:
19116
Exon/intron sizes in nt:
200,127,137,327,67
Flanking exon/introns:
chr2:164844879-164846016|chr2:164865132-164876602
Splice length:
858
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATATTAATAAGGCAGCGGAAAGAAGAAATATGAATACGGCTCCATCAAGACCCAGCCCCACACGAAGGGATCCCTATAGCTTTGGAGATAGTCGCGATACAAGGCGTGATCGCTCTCCAATTCGAGGAAGTCCAAGGAGAGAACCCAGGGATGGCAGAAATGGCCGGGATGCCCGTGATAGCAGAGACATTCGAGATCCTCGGGATTTGCGGGACCGCAGAGACAGCAGGGACATTCGGGATCACAGAGACAGCAGAAGTGTACGTGAAGCCCGGGACCTGAGGGATTTTCGGGACTTTCGTGATCTAAGAGACTCTAGAGATTTTCGAGATCATCGAGACCCTGTGTATGACAGATACAGAGATATAAGAGATTCCAGAGACCCTTTGTACAGGAGAGAAGGCTCATATGACCGTTATCTGAGGGTGGATGACTATTGCCGGAGGAAGGATGACTCGTACTTTGACCGGTACAGAGATAGCTTTGATGGACGGGGCCCTCCTGGCCCAGAGAGTCAATCTCGAGCAAAAGAGCGTTTGAAACGTGAGGAACGACGTAGAGAGGAGCTTTATCGTCGGTATTTTGAGGAGATCCAGCGACGCTTTGATGCTGAGAGGCCTGTGGATTGCTCAGTTATTGTGGTCAACAAACAGACTAAAGACTATGCTGAGTCTGTGGGGAGGAAGGTGCGAGACCTGGGCATGGTCGTGGACCTGATCTTCCTCAACACAGAGGTGTCCCTCTCACAAGCGCTGGAGGATGTCAGCAGAGGAGGCTCTCCCTTCGCCATTGTCATCACCCAGCAGCACCAGATTCACCGTTCCTGCACAGTCAACATCATGTTCGGAACACCACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCGCCATTGTCATCACCCA
59.96
TAGGGATCCCTTCGTGTGGG
60.69
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTGTCATCACCCAGCAGCA
59.96
TAGGGATCCCTTCGTGTGGG
60.69
146
PCR template sequence:
GATGTCAGCAGAGGAGGCTCTCCCTTCGCCATTGTCATCACCCAGCAGCACCAGATTCACCGTTCCTGCACAGTCAACATCATGTTCGGAACACCACAAGATATTAATAAGGCAGCGGAAAGAAGAAATATGAATACGGCTCCATCAAGACCCAGCCCCACACGAAGGGATCCCTATAGCTTTGGAGATAGTCGCGATAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-296-5p(1), mmu-miR-324-5p(1), mmu-miR-326-3p(1), mmu-miR-433-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GGAACACCACAAGATATTAATAAGGC
siRNA sets
CCTTATTAATATCTTGTGG, CTTATTAATATCTTGTGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNCOA5_858_ORF_1
1.0707
0.34005666
0.999965169
1686
ORF sequence:
ATGAATACGGCTCCATCAAGACCCAGCCCCACACGAAGGGATCCCTATAGCTTTGGAGATAGTCGCGATACAAGGCGTGATCGCTCTCCAATTCGAGGAAGTCCAAGGAGAGAACCCAGGGATGGCAGAAATGGCCGGGATGCCCGTGATAGCAGAGACATTCGAGATCCTCGGGATTTGCGGGACCGCAGAGACAGCAGGGACATTCGGGATCACAGAGACAGCAGAAGTGTACGTGAAGCCCGGGACCTGAGGGATTTTCGGGACTTTCGTGATCTAAGAGACTCTAGAGATTTTCGAGATCATCGAGACCCTGTGTATGACAGATACAGAGATATAAGAGATTCCAGAGACCCTTTGTACAGGAGAGAAGGCTCATATGACCGTTATCTGAGGGTGGATGACTATTGCCGGAGGAAGGATGACTCGTACTTTGACCGGTACAGAGATAGCTTTGATGGACGGGGCCCTCCTGGCCCAGAGAGTCAATCTCGAGCAAAAGAGCGTTTGAAACGTGAGGAACGACGTAGAGAGGAGCTTTATCGTCGGTATTTTGAGGAGATCCAGCGACGCTTTGATGCTGAGAGGCCTGTGGATTGCTCAGTTATTGTGGTCAACAAACAGACTAAAGACTATGCTGAGTCTGTGGGGAGGAAGGTGCGAGACCTGGGCATGGTCGTGGACCTGATCTTCCTCAACACAGAGGTGTCCCTCTCACAAGCGCTGGAGGATGTCAGCAGAGGAGGCTCTCCCTTCGCCATTGTCATCACCCAGCAGCACCAGATTCACCGTTCCTGCACAGTCAACATCATGTTCGGAACACCACAAGATATTAATAAGGCAGCGGAAAGAAGAAATATGAATACGGCTCCATCAAGACCCAGCCCCACACGAAGGGATCCCTATAGCTTTGGAGATAGTCGCGATACAAGGCGTGATCGCTCTCCAATTCGAGGAAGTCCAAGGAGAGAACCCAGGGATGGCAGAAATGGCCGGGATGCCCGTGATAGCAGAGACATTCGAGATCCTCGGGATTTGCGGGACCGCAGAGACAGCAGGGACATTCGGGATCACAGAGACAGCAGAAGTGTACGTGAAGCCCGGGACCTGAGGGATTTTCGGGACTTTCGTGATCTAAGAGACTCTAGAGATTTTCGAGATCATCGAGACCCTGTGTATGACAGATACAGAGATATAAGAGATTCCAGAGACCCTTTGTACAGGAGAGAAGGCTCATATGACCGTTATCTGAGGGTGGATGACTATTGCCGGAGGAAGGATGACTCGTACTTTGACCGGTACAGAGATAGCTTTGATGGACGGGGCCCTCCTGGCCCAGAGAGTCAATCTCGAGCAAAAGAGCGTTTGAAACGTGAGGAACGACGTAGAGAGGAGCTTTATCGTCGGTATTTTGAGGAGATCCAGCGACGCTTTGATGCTGAGAGGCCTGTGGATTGCTCAGTTATTGTGGTCAACAAACAGACTAAAGACTATGCTGAGTCTGTGGGGAGGAAGGTGCGAGACCTGGGCATGGTCGTGGACCTGATCTTCCTCAACACAGAGGTGTCCCTCTCACAAGCGCTGGAGGATGTCAGCAGAGGAGGCTCTCCCTTCGCCATTGTCATCACCCAGCAGCACCAGATTCACCGTTCCTGCACAGTCAACATCATGTTCGGAACACCACAA
Peptide sequence:
MNTAPSRPSPTRRDPYSFGDSRDTRRDRSPIRGSPRREPRDGRNGRDARDSRDIRDPRDLRDRRDSRDIRDHRDSRSVREARDLRDFRDFRDLRDSRDFRDHRDPVYDRYRDIRDSRDPLYRREGSYDRYLRVDDYCRRKDDSYFDRYRDSFDGRGPPGPESQSRAKERLKREERRREELYRRYFEEIQRRFDAERPVDCSVIVVNKQTKDYAESVGRKVRDLGMVVDLIFLNTEVSLSQALEDVSRGGSPFAIVITQQHQIHRSCTVNIMFGTPQDINKAAERRNMNTAPSRPSPTRRDPYSFGDSRDTRRDRSPIRGSPRREPRDGRNGRDARDSRDIRDPRDLRDRRDSRDIRDHRDSRSVREARDLRDFRDFRDLRDSRDFRDHRDPVYDRYRDIRDSRDPLYRREGSYDRYLRVDDYCRRKDDSYFDRYRDSFDGRGPPGPESQSRAKERLKREERRREELYRRYFEEIQRRFDAERPVDCSVIVVNKQTKDYAESVGRKVRDLGMVVDLIFLNTEVSLSQALEDVSRGGSPFAIVITQQHQIHRSCTVNIMFGTPQ
IRESfinder result
IRESfinder score
0.295261108
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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