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mmu_circNbeal1_925
Basic information of circular RNA
PanCircBase ID:
mmu_circNbeal1_925
mm39_Chr. Position ID:
chr1|60220621|60256440|+
Gene name:
Nbeal1
Transcript:
ENSMUST00000160834.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
2,3,4,5,6,7,8
Exon/intron offsets:
0,13041,13552,18642,19401,24871,35733
Genomic length:
35819
Exon/intron sizes in nt:
292,92,162,82,128,83,86
Flanking exon/introns:
chr1:60219844-60220621|chr1:60256440-60261225
Splice length:
925
Other database IDs:
circAtlas: mmu-Nbeal1_0102
Human Ortholog IDs:
Mature spliced Sequence:
ACCTATTTACGAGACCAGCTACCCCTGATGAAGAAGTGTTGGAGTAGTTGCTGAAATTGACCCTTATTGACCTGAGACAGTCTTATTTGTTCTACGAATTTTTTTTTTTGTTTTTTTTTTGTTTATTCCACTGCTTTGAGCTTTACTGTAACGGCTGATAAACTTGGAAAATAAAGTAATATGATATGGTCTTGAACCGTCTTTTTGAGGAAATATTAAAGTACCAGAGTGGAAAGCCAGAATGGCAGCCAGAGAGAGGCTCTTTGAACTTTGGATGCTTTATTGTACAAAGAAAGATCCAGATTACCTGAGACTGTGGTTGGACAATTTTGTTTTAAGTTATGAACAATTTTTAGATGTTGACTTTGAAAAGCTGCCTACAAGGGTGGATGATATGCCTCCAGGAATATCTCTGCTTCCTGATAATATTCTGCAGGTTCTGAGGATCCAGCTGCTACACTGTGTTCAGAAAACAGCAGATGGGTTAGAGGAACAACAACAAGCATTGTCAATTTTGCTTGTTAAATTCTTCATTATTCTTTGCAGGAATTTATCAAATGTAGAAGAAATTGGGACTTGCTCTTATATTAATCACGTCATTACCATGACAACACTGTATATTCAGCAATTAAAAAGCAAAAAAAAGGAGAAGGAAATGGCTGATCAGACATCTATTGAAGAATTTGTGATCCATGCACTGGCATTCTGTGAAAGCTTGTATGATCCATATAGAAATTGGAGACATAGAATTTCAGGACGAATCCTTAGTTCTGTGGAAAAGAGCAGACAGAAATACAAACCAGCTTCTCTCACAGTGGAGTTTGTTCCTTTCTTTTATCAATGTTTTCAGGAAAGTGAACATCTCAAGGACAGTCTTAAGTGTTGCTTATTGCATCTCTTTGGAGCCATTGTAGCCGGTGGGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGAACATCTCAAGGACAGTCT
59.1
CTGTCTCAGGTCAATAAGGGTCA
59.48
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGAACATCTCAAGGACAGTCT
59.1
ACTGTCTCAGGTCAATAAGGGTC
59.48
153
PCR template sequence:
TCCTTTCTTTTATCAATGTTTTCAGGAAAGTGAACATCTCAAGGACAGTCTTAAGTGTTGCTTATTGCATCTCTTTGGAGCCATTGTAGCCGGTGGGCAGACCTATTTACGAGACCAGCTACCCCTGATGAAGAAGTGTTGGAGTAGTTGCTGAAATTGACCCTTATTGACCTGAGACAGTCTTATTTGTTCTACGAATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-128-3p(1), mmu-miR-676-3p(1), mmu-miR-499-5p(1), mmu-miR-376a-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGCCGGTGGGCAGACCTATTTACGAG
siRNA sets
CTCGTAAATAGGTCTGCCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNBEAL1_925_ORF_1
0.638
-0.068943413
0.719877636
738
ORF sequence:
ATGGCAGCCAGAGAGAGGCTCTTTGAACTTTGGATGCTTTATTGTACAAAGAAAGATCCAGATTACCTGAGACTGTGGTTGGACAATTTTGTTTTAAGTTATGAACAATTTTTAGATGTTGACTTTGAAAAGCTGCCTACAAGGGTGGATGATATGCCTCCAGGAATATCTCTGCTTCCTGATAATATTCTGCAGGTTCTGAGGATCCAGCTGCTACACTGTGTTCAGAAAACAGCAGATGGGTTAGAGGAACAACAACAAGCATTGTCAATTTTGCTTGTTAAATTCTTCATTATTCTTTGCAGGAATTTATCAAATGTAGAAGAAATTGGGACTTGCTCTTATATTAATCACGTCATTACCATGACAACACTGTATATTCAGCAATTAAAAAGCAAAAAAAAGGAGAAGGAAATGGCTGATCAGACATCTATTGAAGAATTTGTGATCCATGCACTGGCATTCTGTGAAAGCTTGTATGATCCATATAGAAATTGGAGACATAGAATTTCAGGACGAATCCTTAGTTCTGTGGAAAAGAGCAGACAGAAATACAAACCAGCTTCTCTCACAGTGGAGTTTGTTCCTTTCTTTTATCAATGTTTTCAGGAAAGTGAACATCTCAAGGACAGTCTTAAGTGTTGCTTATTGCATCTCTTTGGAGCCATTGTAGCCGGTGGGCAGACCTATTTACGAGACCAGCTACCCCTGATGAAGAAGTGTTGGAGTAGTTGCTGA
Peptide sequence:
MAARERLFELWMLYCTKKDPDYLRLWLDNFVLSYEQFLDVDFEKLPTRVDDMPPGISLLPDNILQVLRIQLLHCVQKTADGLEEQQQALSILLVKFFIILCRNLSNVEEIGTCSYINHVITMTTLYIQQLKSKKKEKEMADQTSIEEFVIHALAFCESLYDPYRNWRHRISGRILSSVEKSRQKYKPASLTVEFVPFFYQCFQESEHLKDSLKCCLLHLFGAIVAGGQTYLRDQLPLMKKCWSSC
IRESfinder result
IRESfinder score
0.863392741
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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