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mmu_circNbas_2548
Basic information of circular RNA
PanCircBase ID:
mmu_circNbas_2548
mm39_Chr. Position ID:
chr12|13429647|13534045|+
Gene name:
Nbas
Transcript:
ENSMUST00000042953.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
17
Exon/intron indexes:
28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44
Exon/intron offsets:
0,11278,13656,25727,28547,33881,35137,35998,53250,61820,62215,73406,73838,90194,94480,102739,104063
Genomic length:
104398
Exon/intron sizes in nt:
1,23,10,32,30,11,31,14,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr12:13427497-13429647|chr12:13534045-13563370
Splice length:
2548
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGTGTCAGAGCTTTTGAAAACACATGGACTGGAGAAACCAGTTTCATTTGTGAAGAACACCCAATCTAGCTCCGAAGAGGCTCGCAGCCTGATGGTTCGCCTGACCAGGCACACTGGGCGGAAGCAGCCTTCTGTTGGTGAGTCTCACTGGAGGATGCTGCTGCAGGACATGTTGACCATGCAGCAGCACGTGTACACGTGCCTCGATTCCAGTGCTTGCTATGAGATATTTACGGAGAGCCTTCTGTGCTCCAGTCGCCTGGAAAACATCCACCTTGCAGGGCAGATGATGCAGTGTAGCGCCTGTTTGGATAATCCTCCAGCTGGTGCGCTCCATAAAGGAAGAGCCCAGTACAGGGTCAGCTATGAGAGAAGCATCGACTTGGTTTTGGCTGCCAGCAGAGAGTACTTCAACTCTTCCACCAACCTCACTGACAGCTGCATGGATTTGGCCAGGTGCTGCTTACAGCTTATAACGGACAGGCCGGCTGCCATTCAGGAGGAATTAGACCTCATTCAAGCCCTGGGGTATCTTGAAGAATTTGGGGTGAAGACCCTGCCTTTGCAAGTACGCTTGTGTTCTGACCGAATCAGTCTCATCAAGGAGTGTGTTTCCCAGTCCCCCACATGCTACAAACAGTCAGCCAAACTGCTAGGCCTTGCTGAGTTGCTGAGAGTTGCAGGTGAAGACCCAGAAGGAAGGCGTGGGCAGGTCCTGATCCTGTTAGTGGAGCAAGCACTCCATGTGCATGACTACAAAGTAGCCAGTATGTACTGTCAGCAGCTGATGGCCGCAGGCTACCCAGAGAGTTGGGCTGTGTGTAGCCAGTTGGGCCAGTCAGAAGGTTACCAGGACCTGGCCACTCGTCAGGAGCTCATGGCTTTCGCTTTGACACATTGCCCTCCCAGCAGCATTGAACTTCTCTTGGAAGCCAGCAGCTCTCTTCAAACAGAGATTCTTTATCAAAGAGTGAACTTTGAGATCCATCCTGAAGGAGAGAATATTAGTGCCTCACCTTTACCTGTTAAAGTTCTACAAGAGGATGCTGCTGCTTTGGGCAGCAGTTCAGCTGACCTGTTACACTGGACCACTGCAACCACCATGAAAGTCTTGTCCAATACCACAACTACTACCAAGGCTGTGCTGCAGGCTGTCAGTGATGGGCAGTGGTGGAAAAAGTCCTTAGCATACCTGCGACCCTACCAGGGGCAGGAATTTGGTAGAGCATATCGAATTGGAATTGTAGCCAATGATGACCTAGAAAGACAAGGATGTCACCCTTTTTATGAGTCAGTCATCGCTAATCCCTTTGTCACCGAGGTCCCTGTGGAAAGCTTTGCTGAAGTGTTGCTGAGAACTGGAAAGCTGACAGAGGCTCAGACACAAGTGTTTCCAACCACGGAAGTTCTCTTGCAACTAGCAAGTGATGCTTTGCCGAATGACATGACATTGGCTCTTGCTTATCTCCTTGCCTTACCTCAGGTGTTGGATGCTAACAAGTGCTTCGAGAAGCAGGCTCATTCGGCACTGTCTCTCCAGCTGGCAGCCTACTACTACAGCCTGCAGATCTATGCCCGATTGGCTCCATGTTTCAGGGACAAGTGCCATCCTCTTTACAGGGTTGATCCCAAGGAATTAATCAAGATGGTTACCGGTCATGTGGCTCAACATGGGCATGAAGGCTGGCCTGAGGACCTCTTGTCCCTGAGCAGGCAACTGCACTACTACAGCGAGCGTCTTCTGGATTTTACCCAGGCTCAGCTCCTCCAGGGCCTGCGGAAGGGCGTGGACGTGCAGAGGTTTACTGCTGATGACCAGTACAAAAGAGAAACCATCCTGGGCCTGGCAGAAAGCCTTGAGGAAAATGTCTACAGCATCGCTCTTTCCCTGGCACAGCGGTACAGTATCTCACAGTGGGAAGTCTTCATGACCCACTTGGAGTTCCTGTTCAGTGAGAGTGGTTTGTCCACAGGAGAAATTGAAAAGCGAGCCCAAACCCTTCACCTCTTTGACACTTTGAAGACAGATCCTGAGTCCTTTCATAAACACATGGTCAAGTATATCTATCCCACCATTGAGGGTCTTGACCATGAAAGGCTGCTGTATTATTTCACTCTCCTGGAAAGCTGTGGTTGTGCCAACTTTGAAACAACTGCTATTAAACCAGAAATCCACATTCGTCTGCTGAAGAAATTTAAAGTTGTTGCATCAGGTCTTAATTATAAGAAGCTGACCGATAAAAGTGAGGATGCGCTGGAAGCCCTGGAGCCTGTGCTCACGAGTCAGAATATCTTATCTATTTCCAAACTCGCCCCCAGAATCCCTGGGAAGCATGGGCAGATGCTTTCCCCCAGCTCTCTGTACACCGTCTGGTTGCAGAAGTTGTTCTGGGCTGGAGATCCGCACCTCATCAAGCAGGCTCCGGAGTCTTCCGCAGAGTGGCTTCATGCCTGTGATGTCTGCCTGAAGTACTTCGATCGACTCTGTCCGGATGACCTCATCACTGTGATGGATGCAATTACATTTTCTCCAAAAGCTGTGTCCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTCGATCGACTCTGTCCGG
59.97
CGAGCCTCTTCGGAGCTAGA
60.82
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCTGTCCGGATGACCTCA
59.96
CGAGCCTCTTCGGAGCTAGA
60.82
151
PCR template sequence:
TGCCTGTGATGTCTGCCTGAAGTACTTCGATCGACTCTGTCCGGATGACCTCATCACTGTGATGGATGCAATTACATTTTCTCCAAAAGCTGTGTCCAAGTGTGTCAGAGCTTTTGAAAACACATGGACTGGAGAAACCAGTTTCATTTGTGAAGAACACCCAATCTAGCTCCGAAGAGGCTCGCAGCCTGATGGTTCGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
28
Interacting miRNAs (#binding sites)
mmu-miR-29b-1-5p(2), mmu-miR-541-5p(2), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-199a-5p(1), mmu-miR-7b-5p(1), mmu-miR-103-3p(1), mmu-miR-205-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-217-5p(1), mmu-miR-7a-5p(1), mmu-miR-326-3p(1), mmu-miR-185-5p(1), mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-128-3p(1), mmu-miR-22-3p(1), mmu-miR-96-5p(1), mmu-miR-183-5p(1), mmu-miR-107-3p(1), mmu-miR-1198-5p(1), mmu-miR-3470b(1), mmu-miR-540-3p(1), mmu-miR-670-5p(1), mmu-miR-697(1), mmu-miR-490-3p(1), mmu-miR-193b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGCTGTGTCCAAGTGTGTCAGAGCTT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNBAS_2548_ORF_1
0.9031
0.217825184
0.999999869
2583
ORF sequence:
ATGCAATTACATTTTCTCCAAAAGCTGTGTCCAAGTGTGTCAGAGCTTTTGAAAACACATGGACTGGAGAAACCAGTTTCATTTGTGAAGAACACCCAATCTAGCTCCGAAGAGGCTCGCAGCCTGATGGTTCGCCTGACCAGGCACACTGGGCGGAAGCAGCCTTCTGTTGGTGAGTCTCACTGGAGGATGCTGCTGCAGGACATGTTGACCATGCAGCAGCACGTGTACACGTGCCTCGATTCCAGTGCTTGCTATGAGATATTTACGGAGAGCCTTCTGTGCTCCAGTCGCCTGGAAAACATCCACCTTGCAGGGCAGATGATGCAGTGTAGCGCCTGTTTGGATAATCCTCCAGCTGGTGCGCTCCATAAAGGAAGAGCCCAGTACAGGGTCAGCTATGAGAGAAGCATCGACTTGGTTTTGGCTGCCAGCAGAGAGTACTTCAACTCTTCCACCAACCTCACTGACAGCTGCATGGATTTGGCCAGGTGCTGCTTACAGCTTATAACGGACAGGCCGGCTGCCATTCAGGAGGAATTAGACCTCATTCAAGCCCTGGGGTATCTTGAAGAATTTGGGGTGAAGACCCTGCCTTTGCAAGTACGCTTGTGTTCTGACCGAATCAGTCTCATCAAGGAGTGTGTTTCCCAGTCCCCCACATGCTACAAACAGTCAGCCAAACTGCTAGGCCTTGCTGAGTTGCTGAGAGTTGCAGGTGAAGACCCAGAAGGAAGGCGTGGGCAGGTCCTGATCCTGTTAGTGGAGCAAGCACTCCATGTGCATGACTACAAAGTAGCCAGTATGTACTGTCAGCAGCTGATGGCCGCAGGCTACCCAGAGAGTTGGGCTGTGTGTAGCCAGTTGGGCCAGTCAGAAGGTTACCAGGACCTGGCCACTCGTCAGGAGCTCATGGCTTTCGCTTTGACACATTGCCCTCCCAGCAGCATTGAACTTCTCTTGGAAGCCAGCAGCTCTCTTCAAACAGAGATTCTTTATCAAAGAGTGAACTTTGAGATCCATCCTGAAGGAGAGAATATTAGTGCCTCACCTTTACCTGTTAAAGTTCTACAAGAGGATGCTGCTGCTTTGGGCAGCAGTTCAGCTGACCTGTTACACTGGACCACTGCAACCACCATGAAAGTCTTGTCCAATACCACAACTACTACCAAGGCTGTGCTGCAGGCTGTCAGTGATGGGCAGTGGTGGAAAAAGTCCTTAGCATACCTGCGACCCTACCAGGGGCAGGAATTTGGTAGAGCATATCGAATTGGAATTGTAGCCAATGATGACCTAGAAAGACAAGGATGTCACCCTTTTTATGAGTCAGTCATCGCTAATCCCTTTGTCACCGAGGTCCCTGTGGAAAGCTTTGCTGAAGTGTTGCTGAGAACTGGAAAGCTGACAGAGGCTCAGACACAAGTGTTTCCAACCACGGAAGTTCTCTTGCAACTAGCAAGTGATGCTTTGCCGAATGACATGACATTGGCTCTTGCTTATCTCCTTGCCTTACCTCAGGTGTTGGATGCTAACAAGTGCTTCGAGAAGCAGGCTCATTCGGCACTGTCTCTCCAGCTGGCAGCCTACTACTACAGCCTGCAGATCTATGCCCGATTGGCTCCATGTTTCAGGGACAAGTGCCATCCTCTTTACAGGGTTGATCCCAAGGAATTAATCAAGATGGTTACCGGTCATGTGGCTCAACATGGGCATGAAGGCTGGCCTGAGGACCTCTTGTCCCTGAGCAGGCAACTGCACTACTACAGCGAGCGTCTTCTGGATTTTACCCAGGCTCAGCTCCTCCAGGGCCTGCGGAAGGGCGTGGACGTGCAGAGGTTTACTGCTGATGACCAGTACAAAAGAGAAACCATCCTGGGCCTGGCAGAAAGCCTTGAGGAAAATGTCTACAGCATCGCTCTTTCCCTGGCACAGCGGTACAGTATCTCACAGTGGGAAGTCTTCATGACCCACTTGGAGTTCCTGTTCAGTGAGAGTGGTTTGTCCACAGGAGAAATTGAAAAGCGAGCCCAAACCCTTCACCTCTTTGACACTTTGAAGACAGATCCTGAGTCCTTTCATAAACACATGGTCAAGTATATCTATCCCACCATTGAGGGTCTTGACCATGAAAGGCTGCTGTATTATTTCACTCTCCTGGAAAGCTGTGGTTGTGCCAACTTTGAAACAACTGCTATTAAACCAGAAATCCACATTCGTCTGCTGAAGAAATTTAAAGTTGTTGCATCAGGTCTTAATTATAAGAAGCTGACCGATAAAAGTGAGGATGCGCTGGAAGCCCTGGAGCCTGTGCTCACGAGTCAGAATATCTTATCTATTTCCAAACTCGCCCCCAGAATCCCTGGGAAGCATGGGCAGATGCTTTCCCCCAGCTCTCTGTACACCGTCTGGTTGCAGAAGTTGTTCTGGGCTGGAGATCCGCACCTCATCAAGCAGGCTCCGGAGTCTTCCGCAGAGTGGCTTCATGCCTGTGATGTCTGCCTGAAGTACTTCGATCGACTCTGTCCGGATGACCTCATCACTGTGATGGATGCAATTACATTTTCTCCAAAAGCTGTGTCCAAG
Peptide sequence:
MQLHFLQKLCPSVSELLKTHGLEKPVSFVKNTQSSSEEARSLMVRLTRHTGRKQPSVGESHWRMLLQDMLTMQQHVYTCLDSSACYEIFTESLLCSSRLENIHLAGQMMQCSACLDNPPAGALHKGRAQYRVSYERSIDLVLAASREYFNSSTNLTDSCMDLARCCLQLITDRPAAIQEELDLIQALGYLEEFGVKTLPLQVRLCSDRISLIKECVSQSPTCYKQSAKLLGLAELLRVAGEDPEGRRGQVLILLVEQALHVHDYKVASMYCQQLMAAGYPESWAVCSQLGQSEGYQDLATRQELMAFALTHCPPSSIELLLEASSSLQTEILYQRVNFEIHPEGENISASPLPVKVLQEDAAALGSSSADLLHWTTATTMKVLSNTTTTTKAVLQAVSDGQWWKKSLAYLRPYQGQEFGRAYRIGIVANDDLERQGCHPFYESVIANPFVTEVPVESFAEVLLRTGKLTEAQTQVFPTTEVLLQLASDALPNDMTLALAYLLALPQVLDANKCFEKQAHSALSLQLAAYYYSLQIYARLAPCFRDKCHPLYRVDPKELIKMVTGHVAQHGHEGWPEDLLSLSRQLHYYSERLLDFTQAQLLQGLRKGVDVQRFTADDQYKRETILGLAESLEENVYSIALSLAQRYSISQWEVFMTHLEFLFSESGLSTGEIEKRAQTLHLFDTLKTDPESFHKHMVKYIYPTIEGLDHERLLYYFTLLESCGCANFETTAIKPEIHIRLLKKFKVVASGLNYKKLTDKSEDALEALEPVLTSQNILSISKLAPRIPGKHGQMLSPSSLYTVWLQKLFWAGDPHLIKQAPESSAEWLHACDVCLKYFDRLCPDDLITVMDAITFSPKAVSK
IRESfinder result
IRESfinder score
0.560301477
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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