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mmu_circMyo9b_893
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo9b_893
mm39_Chr. Position ID:
chr8|71778496|71787038|+
Gene name:
Myo9b
Transcript:
ENSMUST00000212935.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
8,9,10,11,12,13,14
Exon/intron offsets:
0,2027,2693,4855,7401,7774,8460
Genomic length:
8542
Exon/intron sizes in nt:
90,117,135,122,142,205,82
Flanking exon/introns:
chr8:71776637-71778496|chr8:71787038-71791550
Splice length:
893
Other database IDs:
circBase: mmu_circ_0014945, CIRCpedia: MMU_CIRCpedia_27769, circAtlas: mmu-Myo9b_0008
circBase: hsa_circ_0109207, circAtlas: hsa-MYO9B_0054, CIRCpedia: HSA_CIRCpedia_25102
Human Ortholog IDs:
Mature spliced Sequence:
GTAAAGCGGGAGACCCTGGTGGAGGTCTTAACCAAGAGAAAAACAGTTACAGTCAATGACAAACTCATCCTGCCTTACAGCCTCAGTGAGGCCATAACTGCACGAGACTCCATGGCCAAGTCCCTATACAGTGCCCTATTTGACTGGATTGTGCTAAGGATCAACCACGCCCTCCTCAACAAGAAGGACATGGAAGAGGCTGTTTCCTGCTTGTCCATTGGCGTCCTGGACATCTTTGGATTTGAGGACTTCGAAAGGAATAGCTTTGAGCAGTTTTGCATCAACTATGCCAATGAGCAGTTGCAGTACTACTTCACCCAGCACATCTTCAAGCTGGAGCAGGAGGAGTACCAGGGTGAGGGCATCTCGTGGCACAACATTGACTACACCGACAACGTGGGCTGTATCCACCTCATCAGCAAGAAGCCCACTGGCCTCTTCTACCTGCTGGACGAGGAGAGCAACTTCCCACATGCCACAAGCCACACTTTGCTGGCCAAGTTCAAGCAGCAGCATGAGGACAATAAGTACTTCCTGGGCACACCAGTCCTGGAGCCCGCCTTCATCATCCAGCACTTCGCGGGCAGAGTGAAATACCAGATCAAGGACTTCCGGGAGAAGAACATGGACTACATGCGGCCTGACATCGTGGCACTGCTAAGGGGCAGTGACAGCTCCTATGTGCGCCAGCTCATTGGCATGGACCCGGTAGCTGTGTTCCGCTGGGCTGTACTACGAGCAGCCATCAGGGCCATGGCTGTGCTGCGGGAGGCTGGGCGCCTGCGTGCAGAGAGAGCGGAGAAGGCAGCAGCAGGTATAAGTAGCCCTGCCACTCGAAGTCACATGGAAGAGCTACCAAGAGGAGCCAGTACCCCTTCAGAAAAACTGTACCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGCCACTCGAAGTCACAT
60.04
GCTGTAAGGCAGGATGAGTT
57.59
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCACTCGAAGTCACATGGA
60.04
GCTGTAAGGCAGGATGAGTT
57.59
146
PCR template sequence:
GAGCGGAGAAGGCAGCAGCAGGTATAAGTAGCCCTGCCACTCGAAGTCACATGGAAGAGCTACCAAGAGGAGCCAGTACCCCTTCAGAAAAACTGTACCGGTAAAGCGGGAGACCCTGGTGGAGGTCTTAACCAAGAGAAAAACAGTTACAGTCAATGACAAACTCATCCTGCCTTACAGCCTCAGTGAGGCCATAACTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-207(2), mmu-miR-15b-5p(1), mmu-miR-19a-3p(1), mmu-miR-214-3p(1), mmu-miR-149-5p(1), mmu-miR-324-3p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAAACTGTACCGGTAAAGCGGGAGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO9B_893_ORF_1
1.2755
0.524224986
0.992626696
786
ORF sequence:
ATGGCCAAGTCCCTATACAGTGCCCTATTTGACTGGATTGTGCTAAGGATCAACCACGCCCTCCTCAACAAGAAGGACATGGAAGAGGCTGTTTCCTGCTTGTCCATTGGCGTCCTGGACATCTTTGGATTTGAGGACTTCGAAAGGAATAGCTTTGAGCAGTTTTGCATCAACTATGCCAATGAGCAGTTGCAGTACTACTTCACCCAGCACATCTTCAAGCTGGAGCAGGAGGAGTACCAGGGTGAGGGCATCTCGTGGCACAACATTGACTACACCGACAACGTGGGCTGTATCCACCTCATCAGCAAGAAGCCCACTGGCCTCTTCTACCTGCTGGACGAGGAGAGCAACTTCCCACATGCCACAAGCCACACTTTGCTGGCCAAGTTCAAGCAGCAGCATGAGGACAATAAGTACTTCCTGGGCACACCAGTCCTGGAGCCCGCCTTCATCATCCAGCACTTCGCGGGCAGAGTGAAATACCAGATCAAGGACTTCCGGGAGAAGAACATGGACTACATGCGGCCTGACATCGTGGCACTGCTAAGGGGCAGTGACAGCTCCTATGTGCGCCAGCTCATTGGCATGGACCCGGTAGCTGTGTTCCGCTGGGCTGTACTACGAGCAGCCATCAGGGCCATGGCTGTGCTGCGGGAGGCTGGGCGCCTGCGTGCAGAGAGAGCGGAGAAGGCAGCAGCAGGTATAAGTAGCCCTGCCACTCGAAGTCACATGGAAGAGCTACCAAGAGGAGCCAGTACCCCTTCAGAAAAACTGTACCGGTAA
Peptide sequence:
MAKSLYSALFDWIVLRINHALLNKKDMEEAVSCLSIGVLDIFGFEDFERNSFEQFCINYANEQLQYYFTQHIFKLEQEEYQGEGISWHNIDYTDNVGCIHLISKKPTGLFYLLDEESNFPHATSHTLLAKFKQQHEDNKYFLGTPVLEPAFIIQHFAGRVKYQIKDFREKNMDYMRPDIVALLRGSDSSYVRQLIGMDPVAVFRWAVLRAAIRAMAVLREAGRLRAERAEKAAAGISSPATRSHMEELPRGASTPSEKLYR
IRESfinder result
IRESfinder score
0.477297895
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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