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mmu_circMyo7b_564
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo7b_564
mm39_Chr. Position ID:
chr18|32138483|32146498|-
Gene name:
Myo7b
Transcript:
ENSMUST00000134663.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
8,7,6,5
Exon/intron offsets:
0,1570,4621,7830
Genomic length:
8015
Exon/intron sizes in nt:
1,14,14,31,22,185
Flanking exon/introns:
chr18:32136697-32138483|chr18:32146498-32147256
Splice length:
564
Other database IDs:
circAtlas: mmu-Myo7b_0001
Human Ortholog IDs:
Mature spliced Sequence:
ACCTACACAGGCTCCATCCTGGTGGCTGTGAACCCATTCCAGATGCTGCCCCTGTACACCCTGGAGCAGGTACAAATCTACTACAGCCGCCACATGGGCGAGCTGCCTCCCCACATCTTCGCCATTGCCAACAGCTGCTACTTCAACATGAAGAAGAACAAGAGGGACCAGTGCTGTATCATCAGTGGCGAGTCTGGGGCCGGGAAGACCGAGACCACCAAGCTTATCCTTCAGTTCCTCGCCACGGTCAGTGGCCAGCATTCGTGGATCGAGCAACAGGTCCTGGAGGCCAACCCCATCCTGGAAGCCTTTGGCAATGCCAAGACCATCCGCAATGACAACTCCAGCCGCTTCGGGAAGTACATTGACATCCACTTCAATTCTAGTGGGGTGATTGAGGGCGCAAGCATTGAACACTTCCTCCTGGAGAAGTCCAGAGTCTGCCGGCAGGCTCCAGAGGAACGCAACTACCACATCTTCTACTGCATGCTGATGGGGATGAGCCCGGAAGAGAAGCAAATGCTGAGTCTGGGCATGCCCTCTGAGTACCATTACCTGACCATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGATGAGCCCGGAAGAGAAG
59.89
CATGTGGCGGCTGTAGTAGA
59.54
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGATGAGCCCGGAAGAGAAG
59.89
CCATGTGGCGGCTGTAGTAG
60.53
165
PCR template sequence:
CAACTACCACATCTTCTACTGCATGCTGATGGGGATGAGCCCGGAAGAGAAGCAAATGCTGAGTCTGGGCATGCCCTCTGAGTACCATTACCTGACCATGACCTACACAGGCTCCATCCTGGTGGCTGTGAACCCATTCCAGATGCTGCCCCTGTACACCCTGGAGCAGGTACAAATCTACTACAGCCGCCACATGGGCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-182-5p(1), mmu-miR-107-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTACCTGACCATGACCTACACAGGCT
siRNA sets
GTGTAGGTCATGGTCAGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO7B_564_ORF_1
1.2456
0.571116723
0.999267909
1086
ORF sequence:
ATGCTGCCCCTGTACACCCTGGAGCAGGTACAAATCTACTACAGCCGCCACATGGGCGAGCTGCCTCCCCACATCTTCGCCATTGCCAACAGCTGCTACTTCAACATGAAGAAGAACAAGAGGGACCAGTGCTGTATCATCAGTGGCGAGTCTGGGGCCGGGAAGACCGAGACCACCAAGCTTATCCTTCAGTTCCTCGCCACGGTCAGTGGCCAGCATTCGTGGATCGAGCAACAGGTCCTGGAGGCCAACCCCATCCTGGAAGCCTTTGGCAATGCCAAGACCATCCGCAATGACAACTCCAGCCGCTTCGGGAAGTACATTGACATCCACTTCAATTCTAGTGGGGTGATTGAGGGCGCAAGCATTGAACACTTCCTCCTGGAGAAGTCCAGAGTCTGCCGGCAGGCTCCAGAGGAACGCAACTACCACATCTTCTACTGCATGCTGATGGGGATGAGCCCGGAAGAGAAGCAAATGCTGAGTCTGGGCATGCCCTCTGAGTACCATTACCTGACCATGACCTACACAGGCTCCATCCTGGTGGCTGTGAACCCATTCCAGATGCTGCCCCTGTACACCCTGGAGCAGGTACAAATCTACTACAGCCGCCACATGGGCGAGCTGCCTCCCCACATCTTCGCCATTGCCAACAGCTGCTACTTCAACATGAAGAAGAACAAGAGGGACCAGTGCTGTATCATCAGTGGCGAGTCTGGGGCCGGGAAGACCGAGACCACCAAGCTTATCCTTCAGTTCCTCGCCACGGTCAGTGGCCAGCATTCGTGGATCGAGCAACAGGTCCTGGAGGCCAACCCCATCCTGGAAGCCTTTGGCAATGCCAAGACCATCCGCAATGACAACTCCAGCCGCTTCGGGAAGTACATTGACATCCACTTCAATTCTAGTGGGGTGATTGAGGGCGCAAGCATTGAACACTTCCTCCTGGAGAAGTCCAGAGTCTGCCGGCAGGCTCCAGAGGAACGCAACTACCACATCTTCTACTGCATGCTGATGGGGATGAGCCCGGAAGAGAAGCAAATGCTGAGTCTGGGCATGCCCTCTGAGTACCATTACCTGACCATG
Peptide sequence:
MLPLYTLEQVQIYYSRHMGELPPHIFAIANSCYFNMKKNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTIRNDNSSRFGKYIDIHFNSSGVIEGASIEHFLLEKSRVCRQAPEERNYHIFYCMLMGMSPEEKQMLSLGMPSEYHYLTMTYTGSILVAVNPFQMLPLYTLEQVQIYYSRHMGELPPHIFAIANSCYFNMKKNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTIRNDNSSRFGKYIDIHFNSSGVIEGASIEHFLLEKSRVCRQAPEERNYHIFYCMLMGMSPEEKQMLSLGMPSEYHYLTM
IRESfinder result
IRESfinder score
0.370539201
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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