top of page
mmu_circMyo6_555
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo6_555
mm39_Chr. Position ID:
chr9|80136139|80153841|+
Gene name:
Myo6
Transcript:
ENSMUST00000035889.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
5,6,7,8,9
Exon/intron offsets:
0,13307,16277,16782,17537
Genomic length:
17702
Exon/intron sizes in nt:
13,01,06,56,98,165
Flanking exon/introns:
chr9:80133547-80136139|chr9:80153841-80159048
Splice length:
555
Other database IDs:
circAtlas: mmu-Myo6_0017
Human Ortholog IDs:
Mature spliced Sequence:
ACATATGTGGCCAACATTCTGATTGCAGTGAATCCATACTTTGATATACCTAAGATATATTCTTCAGATACAATAAAGTCATATCAAGGAAAATCTCTTGGGACGATGCCACCCCATGTCTTTGCCATTGCTGATAAAGCTTTCCGAGACATGAAGGTGCTCAAGATGAGCCAGTCTATCATCGTATCTGGAGAATCAGGAGCTGGCAAAACAGAAAATACAAAGTTTGTTCTAAGATACCTGACAGAATCTTATGGAACAGGTCAAGATATTGATGATAGAATTGTTGAAGCGAATCCACTTTTAGAAGCATTTGGAAATGCAAAGACTGTTCGCAACAATAATAGCAGTCGATTTGGAAAATTTGTAGAAATTCATTTCAATGAAAAGAGTTCGGTTGTTGGAGGATTTGTTTCCCATTACCTTCTAGAGAAGTCTAGGATCTGTGTTCAAGGCAAAGAGGAGCGGAATTACCATATTTTCTACAGGCTCTGTGCTGGGGCTTCAGAAGACATCAGGGAGAAGCTTCACTTGAGCTCCCCAGATAATTTCCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAAAGAGGAGCGGAATTACCA
58.01
AGTATGGATTCACTGCAATCAGA
57.64
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAAAGAGGAGCGGAATTACCA
58.01
AGTATGGATTCACTGCAATCAGAA
58.25
140
PCR template sequence:
CAAAGAGGAGCGGAATTACCATATTTTCTACAGGCTCTGTGCTGGGGCTTCAGAAGACATCAGGGAGAAGCTTCACTTGAGCTCCCCAGATAATTTCCGGACATATGTGGCCAACATTCTGATTGCAGTGAATCCATACTTTGATATACCTAAGATATATTCTTCAGATACAATAAAGTCATATCAAGGAAAATCTCTTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(1), mmu-miR-495-3p(1), mmu-miR-207(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGATAATTTCCGGACATATGTGGCCA
siRNA sets
ATATGTCCGGAAATTATCT,CATATGTCCGGAAATTATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO6_555_ORF_1
0.9595
0.126426213
0.986407881
1005
ORF sequence:
ATGCCACCCCATGTCTTTGCCATTGCTGATAAAGCTTTCCGAGACATGAAGGTGCTCAAGATGAGCCAGTCTATCATCGTATCTGGAGAATCAGGAGCTGGCAAAACAGAAAATACAAAGTTTGTTCTAAGATACCTGACAGAATCTTATGGAACAGGTCAAGATATTGATGATAGAATTGTTGAAGCGAATCCACTTTTAGAAGCATTTGGAAATGCAAAGACTGTTCGCAACAATAATAGCAGTCGATTTGGAAAATTTGTAGAAATTCATTTCAATGAAAAGAGTTCGGTTGTTGGAGGATTTGTTTCCCATTACCTTCTAGAGAAGTCTAGGATCTGTGTTCAAGGCAAAGAGGAGCGGAATTACCATATTTTCTACAGGCTCTGTGCTGGGGCTTCAGAAGACATCAGGGAGAAGCTTCACTTGAGCTCCCCAGATAATTTCCGGACATATGTGGCCAACATTCTGATTGCAGTGAATCCATACTTTGATATACCTAAGATATATTCTTCAGATACAATAAAGTCATATCAAGGAAAATCTCTTGGGACGATGCCACCCCATGTCTTTGCCATTGCTGATAAAGCTTTCCGAGACATGAAGGTGCTCAAGATGAGCCAGTCTATCATCGTATCTGGAGAATCAGGAGCTGGCAAAACAGAAAATACAAAGTTTGTTCTAAGATACCTGACAGAATCTTATGGAACAGGTCAAGATATTGATGATAGAATTGTTGAAGCGAATCCACTTTTAGAAGCATTTGGAAATGCAAAGACTGTTCGCAACAATAATAGCAGTCGATTTGGAAAATTTGTAGAAATTCATTTCAATGAAAAGAGTTCGGTTGTTGGAGGATTTGTTTCCCATTACCTTCTAGAGAAGTCTAGGATCTGTGTTCAAGGCAAAGAGGAGCGGAATTACCATATTTTCTACAGGCTCTGTGCTGGGGCTTCAGAAGACATCAGGGAGAAGCTTCACTTGAGCTCCCCAGATAATTTCCGG
Peptide sequence:
MPPHVFAIADKAFRDMKVLKMSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDDRIVEANPLLEAFGNAKTVRNNNSSRFGKFVEIHFNEKSSVVGGFVSHYLLEKSRICVQGKEERNYHIFYRLCAGASEDIREKLHLSSPDNFRTYVANILIAVNPYFDIPKIYSSDTIKSYQGKSLGTMPPHVFAIADKAFRDMKVLKMSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDDRIVEANPLLEAFGNAKTVRNNNSSRFGKFVEIHFNEKSSVVGGFVSHYLLEKSRICVQGKEERNYHIFYRLCAGASEDIREKLHLSSPDNFR
IRESfinder result
IRESfinder score
0.656301372
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page