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mmu_circMyo6_1167
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo6_1167
mm39_Chr. Position ID:
chr9|80159048|80181243|+
Gene name:
Myo6
Transcript:
ENSMUST00000035889.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
10,11,12,13,14,15,16,17,18,19
Exon/intron offsets:
0,3113,6646,9012,10507,12425,14359,17860,18227,22156
Genomic length:
22195
Exon/intron sizes in nt:
81,181,145,158,92,73,128,96,174,39
Flanking exon/introns:
chr9:80153841-80159048|chr9:80181243-80183589
Splice length:
1167
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TATTTAAACCGGGGCTGCACTAGATTCTTCGCTAACAAAGAAACGGACAAACAGATTTTACAGAACCGAAAAAGTCCTGAGTATGTTAAGGCAGGTTCCTTGAAGGATCCTCTATTAGACGACCATGGAGATTTTATCAGGATGTGCACAGCCATGAAAAAAATTGGTTTGGATGATGAAGAAAAACTTGATCTGTTTCGAGTTGTAGCTGGTGTCCTGCACCTTGGAAATATTGATTTTGAGGAAGCCGGAAGCACTTCAGGTGGCTGCAATCTGAAGAACAAATCTGCCCCATCCCTGGAATACTGTGCTGAGCTGCTGGGCTTGGACCAAGATGATCTTCGAGTCAGTTTAACCACTCGAGTCATGCTCACCACAGCAGGGGGCACCAAAGGAACCGTTATAAAGGTCCCCCTGAAAGTGGAGCAGGCAAACAATGCTCGCGATGCTCTGGCAAAGACTGTCTATAGCCATCTCTTTGATCACGTGGTGAACAGGGTAAATCAGTGCTTTCCTTTTGAAACATCTTCTTATTTTATTGGAGTCCTGGATATTGCTGGTTTCGAGTACTTTGAGCACAACAGCTTCGAGCAATTTTGTATCAACTATTGCAATGAGAAACTCCAACAGTTTTTTAATGAAAGGATCCTGAAGGAGGAACAAGAACTGTATCAGAAAGAGGGCCTGGGTGTTAATGAAGTCCATTACGTGGACAATCAGGACTGCATAGATTTAATCGAAGTGAAGTTAGTGGGGATCCTGGATATTCTGGATGAAGAAAATCGTCTTCCCCAGCCGAGTGACCAGCACTTCACGTCTGTGGTTCACCAGAAGCACAAAGACCACTTCCGGCTCACTATTCCCAGGAAATCTAAGCTGGCAGTCCACAGGAACCTCAGAGACGATGAAGGCTTTATCATCAGGCACTTTGCAGGGGCCGTGTGCTACGAGACAACCCAGTTTGTGGAGAAAAATAATGATGCACTACATATGTCTCTTGAATCCTTGATATGTGAGTCCAGGGATAAGTTTATAAGGGCATTATTTGAGTCATCTACAAATAACAACAAAGATACGAAACAGAAGGCTGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTCAAGACACAATTAAATTTGCTTCTGGATAAACTTCGAAGTACC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTTCATCAGCGTGGGAAAC
59.83
ACTCAGGACTTTTTCGGTTCTGT
60.12
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTTCATCAGCGTGGGAAAC
59.83
ACCTGCCTTAACATACTCAGGAC
59.8
164
PCR template sequence:
CAAAGATACGAAACAGAAGGCTGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTCAAGACACAATTAAATTTGCTTCTGGATAAACTTCGAAGTACCTATTTAAACCGGGGCTGCACTAGATTCTTCGCTAACAAAGAAACGGACAAACAGATTTTACAGAACCGAAAAAGTCCTGAGTATGTTAAGGCAGGTTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-20b-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-7b-5p(1), mmu-miR-106b-5p(1), mmu-miR-7a-5p(1), mmu-miR-218-5p(1), mmu-miR-370-3p(1), mmu-miR-383-5p(1), mmu-miR-5101(1), mmu-miR-335-3p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
ACTTCGAAGTACCTATTTAAACCGGG
siRNA sets
TTTAAATAGGTACTTCGAA, GTTTAAATAGGTACTTCGA, TAAATAGGTACTTCGAAGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO6_1167_ORF_1
1.2312
0.22239681
0.999998907
2193
ORF sequence:
ATGTGCACAGCCATGAAAAAAATTGGTTTGGATGATGAAGAAAAACTTGATCTGTTTCGAGTTGTAGCTGGTGTCCTGCACCTTGGAAATATTGATTTTGAGGAAGCCGGAAGCACTTCAGGTGGCTGCAATCTGAAGAACAAATCTGCCCCATCCCTGGAATACTGTGCTGAGCTGCTGGGCTTGGACCAAGATGATCTTCGAGTCAGTTTAACCACTCGAGTCATGCTCACCACAGCAGGGGGCACCAAAGGAACCGTTATAAAGGTCCCCCTGAAAGTGGAGCAGGCAAACAATGCTCGCGATGCTCTGGCAAAGACTGTCTATAGCCATCTCTTTGATCACGTGGTGAACAGGGTAAATCAGTGCTTTCCTTTTGAAACATCTTCTTATTTTATTGGAGTCCTGGATATTGCTGGTTTCGAGTACTTTGAGCACAACAGCTTCGAGCAATTTTGTATCAACTATTGCAATGAGAAACTCCAACAGTTTTTTAATGAAAGGATCCTGAAGGAGGAACAAGAACTGTATCAGAAAGAGGGCCTGGGTGTTAATGAAGTCCATTACGTGGACAATCAGGACTGCATAGATTTAATCGAAGTGAAGTTAGTGGGGATCCTGGATATTCTGGATGAAGAAAATCGTCTTCCCCAGCCGAGTGACCAGCACTTCACGTCTGTGGTTCACCAGAAGCACAAAGACCACTTCCGGCTCACTATTCCCAGGAAATCTAAGCTGGCAGTCCACAGGAACCTCAGAGACGATGAAGGCTTTATCATCAGGCACTTTGCAGGGGCCGTGTGCTACGAGACAACCCAGTTTGTGGAGAAAAATAATGATGCACTACATATGTCTCTTGAATCCTTGATATGTGAGTCCAGGGATAAGTTTATAAGGGCATTATTTGAGTCATCTACAAATAACAACAAAGATACGAAACAGAAGGCTGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTCAAGACACAATTAAATTTGCTTCTGGATAAACTTCGAAGTACCTATTTAAACCGGGGCTGCACTAGATTCTTCGCTAACAAAGAAACGGACAAACAGATTTTACAGAACCGAAAAAGTCCTGAGTATGTTAAGGCAGGTTCCTTGAAGGATCCTCTATTAGACGACCATGGAGATTTTATCAGGATGTGCACAGCCATGAAAAAAATTGGTTTGGATGATGAAGAAAAACTTGATCTGTTTCGAGTTGTAGCTGGTGTCCTGCACCTTGGAAATATTGATTTTGAGGAAGCCGGAAGCACTTCAGGTGGCTGCAATCTGAAGAACAAATCTGCCCCATCCCTGGAATACTGTGCTGAGCTGCTGGGCTTGGACCAAGATGATCTTCGAGTCAGTTTAACCACTCGAGTCATGCTCACCACAGCAGGGGGCACCAAAGGAACCGTTATAAAGGTCCCCCTGAAAGTGGAGCAGGCAAACAATGCTCGCGATGCTCTGGCAAAGACTGTCTATAGCCATCTCTTTGATCACGTGGTGAACAGGGTAAATCAGTGCTTTCCTTTTGAAACATCTTCTTATTTTATTGGAGTCCTGGATATTGCTGGTTTCGAGTACTTTGAGCACAACAGCTTCGAGCAATTTTGTATCAACTATTGCAATGAGAAACTCCAACAGTTTTTTAATGAAAGGATCCTGAAGGAGGAACAAGAACTGTATCAGAAAGAGGGCCTGGGTGTTAATGAAGTCCATTACGTGGACAATCAGGACTGCATAGATTTAATCGAAGTGAAGTTAGTGGGGATCCTGGATATTCTGGATGAAGAAAATCGTCTTCCCCAGCCGAGTGACCAGCACTTCACGTCTGTGGTTCACCAGAAGCACAAAGACCACTTCCGGCTCACTATTCCCAGGAAATCTAAGCTGGCAGTCCACAGGAACCTCAGAGACGATGAAGGCTTTATCATCAGGCACTTTGCAGGGGCCGTGTGCTACGAGACAACCCAGTTTGTGGAGAAAAATAATGATGCACTACATATGTCTCTTGAATCCTTGATATGTGAGTCCAGGGATAAGTTTATAAGGGCATTATTTGAGTCATCTACAAATAACAACAAAGATACGAAACAGAAGGCTGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTCAAGACACAATTAAATTTGCTTCTGGATAAACTTCGAAGTACC
Peptide sequence:
MCTAMKKIGLDDEEKLDLFRVVAGVLHLGNIDFEEAGSTSGGCNLKNKSAPSLEYCAELLGLDQDDLRVSLTTRVMLTTAGGTKGTVIKVPLKVEQANNARDALAKTVYSHLFDHVVNRVNQCFPFETSSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQFFNERILKEEQELYQKEGLGVNEVHYVDNQDCIDLIEVKLVGILDILDEENRLPQPSDQHFTSVVHQKHKDHFRLTIPRKSKLAVHRNLRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRALFESSTNNNKDTKQKAGKLSFISVGNKFKTQLNLLLDKLRSTYLNRGCTRFFANKETDKQILQNRKSPEYVKAGSLKDPLLDDHGDFIRMCTAMKKIGLDDEEKLDLFRVVAGVLHLGNIDFEEAGSTSGGCNLKNKSAPSLEYCAELLGLDQDDLRVSLTTRVMLTTAGGTKGTVIKVPLKVEQANNARDALAKTVYSHLFDHVVNRVNQCFPFETSSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQFFNERILKEEQELYQKEGLGVNEVHYVDNQDCIDLIEVKLVGILDILDEENRLPQPSDQHFTSVVHQKHKDHFRLTIPRKSKLAVHRNLRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRALFESSTNNNKDTKQKAGKLSFISVGNKFKTQLNLLLDKLRST
IRESfinder result
IRESfinder score
0.708249875
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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