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mmu_circMyo3a_1129
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo3a_1129
mm39_Chr. Position ID:
chr2|22366839|22438863|+
Gene name:
Myo3a
Transcript:
ENSMUST00000044749.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
16,17,18,19,20,21,22,23,24
Exon/intron offsets:
0,34193,37733,45281,61188,67617,69533,71425,71946
Genomic length:
72024
Exon/intron sizes in nt:
115,127,211,145,154,89,130,80,78
Flanking exon/introns:
chr2:22366502-22366839|chr2:22438863-22448226
Splice length:
1129
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTACTTACAAAATGACAACCTCAGAACAGTACAGGACATGATGAACAATAGTTTCTATAAATCCCAGTATGAATTAATTGAACAATGTTTCAAAGTCATAGGTTTTACAATGGAGCAACTTGGAAGTGTATATAGTGTACTCGCCGCCATCTTGAACGTTGGAAACATTGAATTCTCCTCGGTGGCAACTGAATACCAGATGGACAAGAGCTACATTTGCAATCACACTGCTCTGGAAAACTCCGCTTCTTTGCTTTGCATTCAAGCAGATGAGCTGCAGGAAGCTCTCACCTCACACTGTGTGGTCACTAGAGGAGAAACAATTATTCGACCCAACACAGTGGAAAAGGCTGCTGATGTCCGAGATGCCATGGCCAAAACTTTATATGGCCGTCTCTTTAGCTGGATAGTCAATTGTATTAACAGCTTGCTGAAGCATGACACATCACCTAGTGGGGATGAGGAGCTAAACATCGGCATTCTTGACATATTCGGCTTTGAAAACTTCAAAAGAAATTCCTTTGAGCAGCTGTGCATTAACATTGCAAATGAACAGATCCAGTATTATTTTAATCAGCACGTGTTTGCATGGGAGCAGAATGAATACCTAAATGAAGATGTAGATGCTAGAGTTATTGAATACGAAGACAACCGGCCCCTCTTGGACATGTTTCTACAGAAGCCCATGGGCTTATTGTCTCTGCTTGATGAGGAAAGCAGATTTCCCAAGGCCACTGACCAGACTCTCATAGAAAAATTTGAAGACAACCTGAAGTCACAGTACTTCTGGAGACCCAAAAGAATGGAACTCAGTTTTGGCATTCACCACTATGCAGGAAAAGTCCTCTATAGTGCTAGTGGGTTCTTGGCTAAAAACAGAGACACTTTCCCAACTGATATTGTGCTGCTTTTGAGGTCATCAGAAAACAGTGTAATTCGGCAACTTGTCAACCACCCTCTGACCAAAACAGGTAATCTGCCACTTTCTAAAACTAAAAACATAGTAAACTATCAGATGTGGAATTCAGAGAAATCCACAAACCTGACCAAGGGTGAAACTAGAGATGTCACATGCCATGCCTGTGAAACCACCAATGTGAAAACACAAACGGTCTCATCCTATTTTAGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAATCCACAAACCTGACCA
55.85
CATGTCCTGTACTGTTCTGAGGT
59.74
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAATCCACAAACCTGACCAAG
57.76
CATGTCCTGTACTGTTCTGAGGT
59.74
140
PCR template sequence:
GAAATCCACAAACCTGACCAAGGGTGAAACTAGAGATGTCACATGCCATGCCTGTGAAACCACCAATGTGAAAACACAAACGGTCTCATCCTATTTTAGAGTACTTACAAAATGACAACCTCAGAACAGTACAGGACATGATGAACAATAGTTTCTATAAATCCCAGTATGAATTAATTGAACAATGTTTCAAAGTCATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-429-3p(1), mmu-miR-15b-5p(1), mmu-miR-34b-5p(1), mmu-miR-680(1), mmu-miR-29b-2-5p(1), mmu-miR-369-3p(1), mmu-miR-540-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
ATCCTATTTTAGAGTACTTACAAAAT
siRNA sets
GTAAGTACTCTAAAATAGG, TTTGTAAGTACTCTAAAAT, ATTTTGTAAGTACTCTAAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO3A_1129_ORF_1
0.8654
0.12147623
0.991825956
1107
ORF sequence:
ATGATGAACAATAGTTTCTATAAATCCCAGTATGAATTAATTGAACAATGTTTCAAAGTCATAGGTTTTACAATGGAGCAACTTGGAAGTGTATATAGTGTACTCGCCGCCATCTTGAACGTTGGAAACATTGAATTCTCCTCGGTGGCAACTGAATACCAGATGGACAAGAGCTACATTTGCAATCACACTGCTCTGGAAAACTCCGCTTCTTTGCTTTGCATTCAAGCAGATGAGCTGCAGGAAGCTCTCACCTCACACTGTGTGGTCACTAGAGGAGAAACAATTATTCGACCCAACACAGTGGAAAAGGCTGCTGATGTCCGAGATGCCATGGCCAAAACTTTATATGGCCGTCTCTTTAGCTGGATAGTCAATTGTATTAACAGCTTGCTGAAGCATGACACATCACCTAGTGGGGATGAGGAGCTAAACATCGGCATTCTTGACATATTCGGCTTTGAAAACTTCAAAAGAAATTCCTTTGAGCAGCTGTGCATTAACATTGCAAATGAACAGATCCAGTATTATTTTAATCAGCACGTGTTTGCATGGGAGCAGAATGAATACCTAAATGAAGATGTAGATGCTAGAGTTATTGAATACGAAGACAACCGGCCCCTCTTGGACATGTTTCTACAGAAGCCCATGGGCTTATTGTCTCTGCTTGATGAGGAAAGCAGATTTCCCAAGGCCACTGACCAGACTCTCATAGAAAAATTTGAAGACAACCTGAAGTCACAGTACTTCTGGAGACCCAAAAGAATGGAACTCAGTTTTGGCATTCACCACTATGCAGGAAAAGTCCTCTATAGTGCTAGTGGGTTCTTGGCTAAAAACAGAGACACTTTCCCAACTGATATTGTGCTGCTTTTGAGGTCATCAGAAAACAGTGTAATTCGGCAACTTGTCAACCACCCTCTGACCAAAACAGGTAATCTGCCACTTTCTAAAACTAAAAACATAGTAAACTATCAGATGTGGAATTCAGAGAAATCCACAAACCTGACCAAGGGTGAAACTAGAGATGTCACATGCCATGCCTGTGAAACCACCAATGTGAAAACACAAACGGTCTCATCCTATTTTAGAGTACTTACAAAATGA
Peptide sequence:
MMNNSFYKSQYELIEQCFKVIGFTMEQLGSVYSVLAAILNVGNIEFSSVATEYQMDKSYICNHTALENSASLLCIQADELQEALTSHCVVTRGETIIRPNTVEKAADVRDAMAKTLYGRLFSWIVNCINSLLKHDTSPSGDEELNIGILDIFGFENFKRNSFEQLCINIANEQIQYYFNQHVFAWEQNEYLNEDVDARVIEYEDNRPLLDMFLQKPMGLLSLLDEESRFPKATDQTLIEKFEDNLKSQYFWRPKRMELSFGIHHYAGKVLYSASGFLAKNRDTFPTDIVLLLRSSENSVIRQLVNHPLTKTGNLPLSKTKNIVNYQMWNSEKSTNLTKGETRDVTCHACETTNVKTQTVSSYFRVLTK
IRESfinder result
IRESfinder score
0.619484602
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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