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mmu_circMyo1b_267
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo1b_267
mm39_Chr. Position ID:
chr1|51836157|51836694|-
Gene name:
Myo1b
Transcript:
ENSMUST00000046390.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
11,10
Exon/intron offsets:
0,389
Genomic length:
537
Exon/intron sizes in nt:
1,19,148
Flanking exon/introns:
chr1:51833557-51836157|chr1:51836694-51838690
Splice length:
267
Other database IDs:
circAtlas: hsa-MYO1B_0011
Human Ortholog IDs:
Mature spliced Sequence:
AATGCGATGCAGATCGTGGGCTTTCTGGATCATGAGGCCGAGGCTGTCCTGGAGGTAGTGGCAGCCGTTTTGAAACTGGGGAACATCGAGTTCAAGCCTGAATCACGAGTGAATGGTTTGGATGAAAGCAAAATCAAAGATAAAAATGAGTTAAAAGAAATTTGCGAGTTGACCAGCATCGATCAGGTGGTTTTAGAAAGAGCGTTCAGTTTCCGAACAGTTGAAGCGAAGCAGGAGAAAGTTTCAACCACGCTGAATGTGGCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGCATCGATCAGGTGGTT
60.11
CCCCAGTTTCAAAACGGCTG
59.97
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGACCAGCATCGATCAGGTG
59.82
CCCCAGTTTCAAAACGGCTG
59.97
179
PCR template sequence:
GTTGACCAGCATCGATCAGGTGGTTTTAGAAAGAGCGTTCAGTTTCCGAACAGTTGAAGCGAAGCAGGAGAAAGTTTCAACCACGCTGAATGTGGCTCAGAATGCGATGCAGATCGTGGGCTTTCTGGATCATGAGGCCGAGGCTGTCCTGGAGGTAGTGGCAGCCGTTTTGAAACTGGGGAACATCGAGTTCAAGCCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-181a-5p(1), mmu-miR-181b-5p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAATGTGGCTCAGAATGCGATGCAGA
siRNA sets
TCTGCATCGCATTCTGAGC,ATCGCATTCTGAGCCACAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO1B_267_ORF_1
1.1124
0.405700706
0.907376771
528
ORF sequence:
ATGCAGATCGTGGGCTTTCTGGATCATGAGGCCGAGGCTGTCCTGGAGGTAGTGGCAGCCGTTTTGAAACTGGGGAACATCGAGTTCAAGCCTGAATCACGAGTGAATGGTTTGGATGAAAGCAAAATCAAAGATAAAAATGAGTTAAAAGAAATTTGCGAGTTGACCAGCATCGATCAGGTGGTTTTAGAAAGAGCGTTCAGTTTCCGAACAGTTGAAGCGAAGCAGGAGAAAGTTTCAACCACGCTGAATGTGGCTCAGAATGCGATGCAGATCGTGGGCTTTCTGGATCATGAGGCCGAGGCTGTCCTGGAGGTAGTGGCAGCCGTTTTGAAACTGGGGAACATCGAGTTCAAGCCTGAATCACGAGTGAATGGTTTGGATGAAAGCAAAATCAAAGATAAAAATGAGTTAAAAGAAATTTGCGAGTTGACCAGCATCGATCAGGTGGTTTTAGAAAGAGCGTTCAGTTTCCGAACAGTTGAAGCGAAGCAGGAGAAAGTTTCAACCACGCTGAATGTGGCTCAG
Peptide sequence:
MQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQ
IRESfinder result
IRESfinder score
0.565487323
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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