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mmu_circMir670hg_357
Basic information of circular RNA
PanCircBase ID:
mmu_circMir670hg_357
mm39_Chr. Position ID:
chr2|93997188|94068732|-
Gene name:
Mir670hg
Transcript:
ENSMUST00000210851.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,17723,71429
Genomic length:
71544
Exon/intron sizes in nt:
147,95,115
Flanking exon/introns:
chr2:93991553-93997188|chr2:94068732-94076408
Splice length:
357
Other database IDs:
circAtlas: mmu-Gm23630_0001
Human Ortholog IDs:
Mature spliced Sequence:
GTGGTCAAGCCCGTCCTGATACTGAATGGTACAGTGAGGAGTGGCCACCCAAGGGAGGCAGTCTCGGAGGTCACAGGAGCTGATAAGCCTGGATGAAAGATCACAGGAGTTCCAGGAACTCCGTTACTTGCATGGAGAGGAAGTTTGCAAGAGAATTTGGCAGTTCTGCTGGATCAAGCAAATAAAGATTGGCAACTGGAGCTAGAGCTGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGGCTGCTCTTCCAGAGGATCCAGGTTCAATTTCCAGGACCCATATAGCAGCTCACAGCTGTCTGTAACTCCAGATCCAGAGGATCCGACATGCTCACACAGACTTACACTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCCATATAGCAGCTCACAGC
59.93
CTCCTGTGACCTCCGAGACT
60.32
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAGGACCCATATAGCAGCT
60.13
CTCCTGTGACCTCCGAGACT
60.32
161
PCR template sequence:
GAGGATCCAGGTTCAATTTCCAGGACCCATATAGCAGCTCACAGCTGTCTGTAACTCCAGATCCAGAGGATCCGACATGCTCACACAGACTTACACTCAGGTGGTCAAGCCCGTCCTGATACTGAATGGTACAGTGAGGAGTGGCCACCCAAGGGAGGCAGTCTCGGAGGTCACAGGAGCTGATAAGCCTGGATGAAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-127-3p(1), mmu-miR-27a-5p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACTTACACTCAGGTGGTCAAGCCCG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMIR670HG_357_ORF_1
0.9956
0.139860274
0.122328876
120
ORF sequence:
ATGCTCACACAGACTTACACTCAGGTGGTCAAGCCCGTCCTGATACTGAATGGTACAGTGAGGAGTGGCCACCCAAGGGAGGCAGTCTCGGAGGTCACAGGAGCTGATAAGCCTGGATGA
Peptide sequence:
MLTQTYTQVVKPVLILNGTVRSGHPREAVSEVTGADKPG
IRESfinder result
IRESfinder score
0.522827041
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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