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mmu_circMia2_753
Basic information of circular RNA
PanCircBase ID:
mmu_circMia2_753
mm39_Chr. Position ID:
chr12|59182849|59201264|+
Gene name:
Mia2
Transcript:
ENSMUST00000219140.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
7,8,9,10,11,12,13,14,15
Exon/intron offsets:
0,1517,1634,8635,10272,10783,11788,13400,18292
Genomic length:
18415
Exon/intron sizes in nt:
1,32,22,89,72,27,11,17,00,00,000
Flanking exon/introns:
chr12:59166593-59182849|chr12:59201264-59205038
Splice length:
753
Other database IDs:
CIRCpedia: MMU_CIRCpedia_217041, circAtlas: mmu-Ctage5_0023
circAtlas: hsa-RP11-407N17_0027, CIRCpedia: HSA_CIRCpedia_120917
Human Ortholog IDs:
Mature spliced Sequence:
GTTGTGGCAGCATTGCCTGAAGATATGAGAGCAGACTTTAATCCCAGTGGTTTTTCGTTGGAATTGGCAGTATGTGTGCTTTCCGTTGGACTGCTTGCCGTGGTCTTGTTTCTGTGGCGAGGCTTCCGATCAATTCGAAGCCGATTTTATGTGGGGAGAGAAAAGAAACTTGCTCTTGAGCTTTCTGCACTAATTGAAGAAAAATGTAAACTGCTTGACAAAGTCAGCATTGTTCAAAAGGAGTATGAAGGCTTAGAGTCATCTTTAAAGGAGGCCAGCTTTGAGAAGGAGTCAACAGAAGCACAAAGTTTGGAGTTTGTTGAAGGATCACAAATATCAGAGGCCACCTATGAAAATCTGGAACAATCCAAATCTAAACTTGAAGATGAAATACTACTCCTAGAGGAAAAGCTGGAAGAAGAGCGAGCTAAACATTCTGAACAAGACGAGCTGATGGCTGATATTTCAAAAAGGATACAGTCCCTAGAAGATGAATCAAAATCTCTCAAATCGCAAGTAGCTGAAGCCAAAACAACCTTTAGAATATTTGAAATCAATGAAGAGCGACTCAAGGGAGCAATAAAAGATGCCTTGAATGAAAATTCTCAGCTTCAGGAAAGCCAGAAACAGCTTTTACAGGAAACTGAAATGATGAAAGAACAAGTGAATGATCTCGATAAACAGAAAGTGGCATTGGAAGAGTCCAGAGCACAGGCGGAACAAGCTCTGAGTGAGAAGGAGAGTCAGATTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAGAGTCCAGAGCACAGG
59.75
CAAGCAGTCCAACGGAAAGC
60.04
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAGAGTCCAGAGCACAGG
59.75
GCAAGCAGTCCAACGGAAAG
60.04
156
PCR template sequence:
GAAAGAACAAGTGAATGATCTCGATAAACAGAAAGTGGCATTGGAAGAGTCCAGAGCACAGGCGGAACAAGCTCTGAGTGAGAAGGAGAGTCAGATTGAGGTTGTGGCAGCATTGCCTGAAGATATGAGAGCAGACTTTAATCCCAGTGGTTTTTCGTTGGAATTGGCAGTATGTGTGCTTTCCGTTGGACTGCTTGCCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-335-5p(1), mmu-miR-326-3p(1), mmu-miR-191-5p(1), mmu-miR-127-5p(1), mmu-miR-335-3p(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGTCAGATTGAGGTTGTGGCAGCAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMIA2_753_ORF_1
1.1831
0.178402842
0.999762679
1482
ORF sequence:
ATGAGAGCAGACTTTAATCCCAGTGGTTTTTCGTTGGAATTGGCAGTATGTGTGCTTTCCGTTGGACTGCTTGCCGTGGTCTTGTTTCTGTGGCGAGGCTTCCGATCAATTCGAAGCCGATTTTATGTGGGGAGAGAAAAGAAACTTGCTCTTGAGCTTTCTGCACTAATTGAAGAAAAATGTAAACTGCTTGACAAAGTCAGCATTGTTCAAAAGGAGTATGAAGGCTTAGAGTCATCTTTAAAGGAGGCCAGCTTTGAGAAGGAGTCAACAGAAGCACAAAGTTTGGAGTTTGTTGAAGGATCACAAATATCAGAGGCCACCTATGAAAATCTGGAACAATCCAAATCTAAACTTGAAGATGAAATACTACTCCTAGAGGAAAAGCTGGAAGAAGAGCGAGCTAAACATTCTGAACAAGACGAGCTGATGGCTGATATTTCAAAAAGGATACAGTCCCTAGAAGATGAATCAAAATCTCTCAAATCGCAAGTAGCTGAAGCCAAAACAACCTTTAGAATATTTGAAATCAATGAAGAGCGACTCAAGGGAGCAATAAAAGATGCCTTGAATGAAAATTCTCAGCTTCAGGAAAGCCAGAAACAGCTTTTACAGGAAACTGAAATGATGAAAGAACAAGTGAATGATCTCGATAAACAGAAAGTGGCATTGGAAGAGTCCAGAGCACAGGCGGAACAAGCTCTGAGTGAGAAGGAGAGTCAGATTGAGGTTGTGGCAGCATTGCCTGAAGATATGAGAGCAGACTTTAATCCCAGTGGTTTTTCGTTGGAATTGGCAGTATGTGTGCTTTCCGTTGGACTGCTTGCCGTGGTCTTGTTTCTGTGGCGAGGCTTCCGATCAATTCGAAGCCGATTTTATGTGGGGAGAGAAAAGAAACTTGCTCTTGAGCTTTCTGCACTAATTGAAGAAAAATGTAAACTGCTTGACAAAGTCAGCATTGTTCAAAAGGAGTATGAAGGCTTAGAGTCATCTTTAAAGGAGGCCAGCTTTGAGAAGGAGTCAACAGAAGCACAAAGTTTGGAGTTTGTTGAAGGATCACAAATATCAGAGGCCACCTATGAAAATCTGGAACAATCCAAATCTAAACTTGAAGATGAAATACTACTCCTAGAGGAAAAGCTGGAAGAAGAGCGAGCTAAACATTCTGAACAAGACGAGCTGATGGCTGATATTTCAAAAAGGATACAGTCCCTAGAAGATGAATCAAAATCTCTCAAATCGCAAGTAGCTGAAGCCAAAACAACCTTTAGAATATTTGAAATCAATGAAGAGCGACTCAAGGGAGCAATAAAAGATGCCTTGAATGAAAATTCTCAGCTTCAGGAAAGCCAGAAACAGCTTTTACAGGAAACTGAAATGATGAAAGAACAAGTGAATGATCTCGATAAACAGAAAGTGGCATTGGAAGAGTCCAGAGCACAGGCGGAACAAGCTCTGAGTGAGAAGGAGAGTCAGATTGAG
Peptide sequence:
MRADFNPSGFSLELAVCVLSVGLLAVVLFLWRGFRSIRSRFYVGREKKLALELSALIEEKCKLLDKVSIVQKEYEGLESSLKEASFEKESTEAQSLEFVEGSQISEATYENLEQSKSKLEDEILLLEEKLEEERAKHSEQDELMADISKRIQSLEDESKSLKSQVAEAKTTFRIFEINEERLKGAIKDALNENSQLQESQKQLLQETEMMKEQVNDLDKQKVALEESRAQAEQALSEKESQIEVVAALPEDMRADFNPSGFSLELAVCVLSVGLLAVVLFLWRGFRSIRSRFYVGREKKLALELSALIEEKCKLLDKVSIVQKEYEGLESSLKEASFEKESTEAQSLEFVEGSQISEATYENLEQSKSKLEDEILLLEEKLEEERAKHSEQDELMADISKRIQSLEDESKSLKSQVAEAKTTFRIFEINEERLKGAIKDALNENSQLQESQKQLLQETEMMKEQVNDLDKQKVALEESRAQAEQALSEKESQIE
IRESfinder result
IRESfinder score
0.634670974
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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