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mmu_circMbtps2_586
Basic information of circular RNA
PanCircBase ID:
mmu_circMbtps2_586
mm39_Chr. Position ID:
chrX|156333927|156338687|-
Gene name:
Mbtps2
Transcript:
ENSMUST00000065857.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
8,7,6,5
Exon/intron offsets:
0,1984,3951,4665
Genomic length:
4760
Exon/intron sizes in nt:
219,76,196,95
Flanking exon/introns:
chrX:156318469-156333927|chrX:156338687-156341880
Splice length:
586
Other database IDs:
circAtlas: mmu-Mbtps2_0003
circAtlas: hsa-MBTPS2_0006
Human Ortholog IDs:
Mature spliced Sequence:
CATACAAACGACTAGATGGTTCTACTGAATGCTGTAACAATCACAGCCTCACAGATGTGTGCTTTTCCTACAGAAATAATTTCAATAAACGTTTGCATACGTGTCTACCTGCCCGGAAAGCAGTTGAAGCTACTCAAGTTTGTAGAAGCAATAAAGATTGCAAAAGCGGCGCAAGTTCTAGCTTCTGTATAGTACCGTCCTTGGAAACTCACACCCGACTAATCAAAGTGAAACATCCCCCGCAAATTGATATGCTGTATGTAGGGCACCCACTGCATCTTCACTACACAGTGAGCATTACAAGTTTCATCCCACGTTTCAACTTTCTAAGCATAGATCTGCCAGTGATTGTGGAGACATTTGTCAAGTACCTGATTTCTCTTTCTGGAGCTCTGGCTATTGTTAATGCAGTGCCCTGCTTTGCCTTGGATGGTCAATGGATTTTAAACTCTTTCCTGGATGCTACCCTTACTTCAGTAATTGGAGACAATGATGTCAAAGATCTGATTGGATTTTTCATCTTGCTTGGTGGCAGTGTACTTTTGGCTGCCAATGTGACACTGGGACTCTGGATGGTTACAGCACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCATCTTGCTTGGTGGCAGT
59.89
CTGTAGGAAAAGCACACATCTGT
58.93
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCATCTTGCTTGGTGGCAGT
59.89
TGTAGGAAAAGCACACATCTGT
57.91
142
PCR template sequence:
ACAATGATGTCAAAGATCTGATTGGATTTTTCATCTTGCTTGGTGGCAGTGTACTTTTGGCTGCCAATGTGACACTGGGACTCTGGATGGTTACAGCACGCATACAAACGACTAGATGGTTCTACTGAATGCTGTAACAATCACAGCCTCACAGATGTGTGCTTTTCCTACAGAAATAATTTCAATAAACGTTTGCATAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-137-3p(1), mmu-miR-23b-3p(1), mmu-miR-155-5p(1), mmu-miR-217-5p(1), mmu-miR-23a-3p(1), mmu-miR-324-3p(1), mmu-miR-539-5p(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGTTACAGCACGCATACAAACGACT
siRNA sets
TTTGTATGCGTGCTGTAAC, TTGTATGCGTGCTGTAACC, TGTATGCGTGCTGTAACCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMBTPS2_586_ORF_1
0.9429
-0.000819681
0.288871581
363
ORF sequence:
ATGCTGTATGTAGGGCACCCACTGCATCTTCACTACACAGTGAGCATTACAAGTTTCATCCCACGTTTCAACTTTCTAAGCATAGATCTGCCAGTGATTGTGGAGACATTTGTCAAGTACCTGATTTCTCTTTCTGGAGCTCTGGCTATTGTTAATGCAGTGCCCTGCTTTGCCTTGGATGGTCAATGGATTTTAAACTCTTTCCTGGATGCTACCCTTACTTCAGTAATTGGAGACAATGATGTCAAAGATCTGATTGGATTTTTCATCTTGCTTGGTGGCAGTGTACTTTTGGCTGCCAATGTGACACTGGGACTCTGGATGGTTACAGCACGCATACAAACGACTAGATGGTTCTACTGA
Peptide sequence:
MLYVGHPLHLHYTVSITSFIPRFNFLSIDLPVIVETFVKYLISLSGALAIVNAVPCFALDGQWILNSFLDATLTSVIGDNDVKDLIGFFILLGGSVLLAANVTLGLWMVTARIQTTRWFY
IRESfinder result
IRESfinder score
0.777672677
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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