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mmu_circMapk10_372
Basic information of circular RNA
PanCircBase ID:
mmu_circMapk10_372
mm39_Chr. Position ID:
chr5|103076088|103114494|-
Gene name:
Mapk10
Transcript:
ENSMUST00000170792.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
12,11,10
Exon/intron offsets:
0,35244,38223
Genomic length:
38406
Exon/intron sizes in nt:
6,41,25,183
Flanking exon/introns:
chr5:103074015-103076088|chr5:103114494-103135068
Splice length:
372
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATATTGACCAGTGGAACAAAGTCATCGAGCAGCTAGGAACTCCGTGTCCAGAGTTCATGAAGAAATTGCAGCCCACAGTCAGAAACTACGTGGAGAATCGGCCCAAGTACGCAGGACTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTCCCAGCGGATTCTGAGCACAATAAACTTAAAGCCAGCCAAGCCAGGGATTTGTTGTCTAAGATGTTAGTGATTGACCCAGCGAAGAGGATATCGGTGGACGACGCACTGCAGCATCCGTACATCAACGTTTGGTACGACCCGGCTGAAGTGGAGGCGCCTCCGCCTCAGATATATGATAAGCAGCTGGATGAAAGGGAGCACACCATCGAAGAATGGAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGCCTCCGCCTCAGATATAT
58.89
TTTCTGACTGTGGGCTGCAA
60.11
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGCCTCCGCCTCAGATATAT
58.89
TCTGACTGTGGGCTGCAATT
59.89
149
PCR template sequence:
ATCAACGTTTGGTACGACCCGGCTGAAGTGGAGGCGCCTCCGCCTCAGATATATGATAAGCAGCTGGATGAAAGGGAGCACACCATCGAAGAATGGAAAGATATTGACCAGTGGAACAAAGTCATCGAGCAGCTAGGAACTCCGTGTCCAGAGTTCATGAAGAAATTGCAGCCCACAGTCAGAAACTACGTGGAGAATCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-3065-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGAATGGAAAGATATTGACCAGTG
siRNA sets
CACTGGTCAATATCTTTCC, ACTGGTCAATATCTTTCCA, CAATATCTTTCCATTCTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAPK10_372_ORF_1
1.0344
0.377857765
0.959519508
687
ORF sequence:
ATGAAGAAATTGCAGCCCACAGTCAGAAACTACGTGGAGAATCGGCCCAAGTACGCAGGACTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTCCCAGCGGATTCTGAGCACAATAAACTTAAAGCCAGCCAAGCCAGGGATTTGTTGTCTAAGATGTTAGTGATTGACCCAGCGAAGAGGATATCGGTGGACGACGCACTGCAGCATCCGTACATCAACGTTTGGTACGACCCGGCTGAAGTGGAGGCGCCTCCGCCTCAGATATATGATAAGCAGCTGGATGAAAGGGAGCACACCATCGAAGAATGGAAAGATATTGACCAGTGGAACAAAGTCATCGAGCAGCTAGGAACTCCGTGTCCAGAGTTCATGAAGAAATTGCAGCCCACAGTCAGAAACTACGTGGAGAATCGGCCCAAGTACGCAGGACTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTCCCAGCGGATTCTGAGCACAATAAACTTAAAGCCAGCCAAGCCAGGGATTTGTTGTCTAAGATGTTAGTGATTGACCCAGCGAAGAGGATATCGGTGGACGACGCACTGCAGCATCCGTACATCAACGTTTGGTACGACCCGGCTGAAGTGGAGGCGCCTCCGCCTCAGATATATGATAAGCAGCTGGATGAAAGGGAGCACACCATCGAAGAATGGAAA
Peptide sequence:
MKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKDIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWK
IRESfinder result
IRESfinder score
0.443620529
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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