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mmu_circLars_1877
Basic information of circular RNA
PanCircBase ID:
mmu_circLars_1877
mm39_Chr. Position ID:
chr18|42361070|42383974|-
Gene name:
Lars
Transcript:
ENSMUST00000097590.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
17
Exon/intron indexes:
20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,652,1623,1785,1936,5794,6575,7479,8577,8733,13897,14698,14961,15771,16743,17934,22823
Genomic length:
22904
Exon/intron sizes in nt:
213,139,83,66,86,78,141,54,77,88,226,68,64,113,162,138,81
Flanking exon/introns:
chr18:42360358-42361070|chr18:42383974-42384357
Splice length:
1877
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTTGCAGTAGGATACCAGAGATTAAAAGGAAAAAGCTGTCTGTTTCCCTTTGGGTTGCACTGTACTGGTATGCCTATTAAGGCATGCGCTGACAAGCTGAAAAGAGAAATAGAGCTGTATGGCTGCCCTCCTGATTTTCCCGAGGAAGAAGAGGAAGAGGAAGAAAGCAGTGCTAAACCTGGAGATATAGTAGTGAGGGATAAAGCCAAAGGGAAAAAGAGCAAAGCTGCCGCCAAAGCAGGATCTTCTAAGTACCAGTGGGACATTATGAAATCCCTTGGGCTCTCTGATGACGACATAGTGAAGTTTTCTGAAGCAGAGCACTGGCTTGATTATTTCCCACCACTGGCCGTTCAGGATCTGAAGACTATAGGTTTGAAGGTAGACTGGCGGCGCTCCTTCATCACCACGGACGTGAATCCTTACTACGATTCCTTTGTCAGGTGGCAGTTTTTAACTTTAAGAGAAAGAAATAAAATTAAATTTGGGAAGCGGTACACTATTTATTCCCCAAAAGATGGACAGCCTTGCATGGATCATGACAGACAAACTGGAGAGGGTGTTGGACCTCAGGAATATACTCTGGTCAAACTGAAGGTGCTTGAACCATATCCATCAAAATTAAGCGGCCTGAAAGGGAAAAATATCTTCCTAGTAGCTGCTACACTCAGACCTGAGACTATGTTTGGGCAGACAAATTGTTGGGTTCGGCCTGATATGAAATACATTGGGTTTGAGACAGCAAATGGAGACATCTTCATCTGTACCCAGAGAGCAGCCAGGAATATGTCATACCAAGGCTTCACCAAGCACAACGGAGTGGTGCCGGTTGTGAAGGAGTTAATGGGAGAGGAAATCCTTGGGGCTTCACTTTCTGCACCCCTGACGTGCTATAAGGTGGTCTACGTTCTCCCTATGCTGACCATTAAAGAGGATAAAGGCACTGGGGTGGTCACGAGCGTTCCTTCTGATTCCCCGGATGATCTTGCAGCACTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAACAAAGTTTGGAATTAGGGATGACATGGTCCTGCCGTTTGAACCGGTGCCAGTCCTGGAAATCCCAGGGATTGGAAATCTTCCTGCTGTGACTGTTTGTGATGAGCTGAAAATTCAGAGCCAGAATGACCGTGAAAAACTTGCAGAAGCAAAGGAGAAATTGTATTTAAGAGGATTTTATGATGGTGTAATGCTAGTGGATGGCTTTAAAGGACAGAAGATCCAACATGTAAAGAAGACTATTCAGAAAAACATGATTGATGCTGGAGATGCGCTTATCTACATGGAGCCAGAAAAACAAGTGATGTCGAGGTCTGCAGATGAGTGCGTCGTGGCCCTGTGTGACCAGTGGTACTTGGATTATGGAGATGAGAACTGGAAGAAACAGACATTTCAGTGCCTGAAGAACATGGAAACATTCTGCGAGGAGAGCAGGAAGAATTTTGAAGCTTCCTTAGACTGGCTACAAGAACATGCTTGCTCAAGAACGTACGGTTTAGGCACTCGTCTGCCTTGGGATGAACAGTGGTTAATCGAATCTCTCTCGGATTCCACAATTTACATGGCGTTCTACACAGTGGCACACCTGCTGCAGGGGGGTGACCTGAATGGGCAGGCGGAGTCTCCACTGGGCATCAGACCCCAGCAGATGACAAAGGACGTTTGGGATTATGTGTTCTTCAAGGATGCTCCATTTCCTAAGACTCAGATCCCAAAGGAGAAGCTGGATCAGTTGAAGCAGGAGTTTGAGTTTTGGTACCCAGTGGACCTTCGAGCCTCTGGCAAGGATCTGATTCCAAATCATCTTTCATATTACATTTACAACCATGTGGCCATGTGGCCAGAGCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTACCCAGTGGACCTTCGAG
59.47
ACCAGTACAGTGCAACCCAA
59.45
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTACCCAGTGGACCTTCGA
60.9
ACCAGTACAGTGCAACCCAA
59.45
166
PCR template sequence:
TTTGGTACCCAGTGGACCTTCGAGCCTCTGGCAAGGATCTGATTCCAAATCATCTTTCATATTACATTTACAACCATGTGGCCATGTGGCCAGAGCAAAGTTTGCAGTAGGATACCAGAGATTAAAAGGAAAAAGCTGTCTGTTTCCCTTTGGGTTGCACTGTACTGGTATGCCTATTAAGGCATGCGCTGACAAGCTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(2), mmu-miR-9-5p(1), mmu-miR-375-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-130a-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-132-3p(1), mmu-miR-203-3p(1), mmu-miR-136-5p(1), mmu-miR-22-3p(1), mmu-miR-130b-3p(1), mmu-miR-721(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-362-5p(1), mmu-miR-377-3p(1), mmu-miR-207(1), mmu-miR-300-3p(1), mmu-miR-532-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCCAGAGCAAAGTTTGCAGTAGGAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCLARS_1877_ORF_1
0.9186
0.213071714
0.999968575
1818
ORF sequence:
ATGCCTATTAAGGCATGCGCTGACAAGCTGAAAAGAGAAATAGAGCTGTATGGCTGCCCTCCTGATTTTCCCGAGGAAGAAGAGGAAGAGGAAGAAAGCAGTGCTAAACCTGGAGATATAGTAGTGAGGGATAAAGCCAAAGGGAAAAAGAGCAAAGCTGCCGCCAAAGCAGGATCTTCTAAGTACCAGTGGGACATTATGAAATCCCTTGGGCTCTCTGATGACGACATAGTGAAGTTTTCTGAAGCAGAGCACTGGCTTGATTATTTCCCACCACTGGCCGTTCAGGATCTGAAGACTATAGGTTTGAAGGTAGACTGGCGGCGCTCCTTCATCACCACGGACGTGAATCCTTACTACGATTCCTTTGTCAGGTGGCAGTTTTTAACTTTAAGAGAAAGAAATAAAATTAAATTTGGGAAGCGGTACACTATTTATTCCCCAAAAGATGGACAGCCTTGCATGGATCATGACAGACAAACTGGAGAGGGTGTTGGACCTCAGGAATATACTCTGGTCAAACTGAAGGTGCTTGAACCATATCCATCAAAATTAAGCGGCCTGAAAGGGAAAAATATCTTCCTAGTAGCTGCTACACTCAGACCTGAGACTATGTTTGGGCAGACAAATTGTTGGGTTCGGCCTGATATGAAATACATTGGGTTTGAGACAGCAAATGGAGACATCTTCATCTGTACCCAGAGAGCAGCCAGGAATATGTCATACCAAGGCTTCACCAAGCACAACGGAGTGGTGCCGGTTGTGAAGGAGTTAATGGGAGAGGAAATCCTTGGGGCTTCACTTTCTGCACCCCTGACGTGCTATAAGGTGGTCTACGTTCTCCCTATGCTGACCATTAAAGAGGATAAAGGCACTGGGGTGGTCACGAGCGTTCCTTCTGATTCCCCGGATGATCTTGCAGCACTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAACAAAGTTTGGAATTAGGGATGACATGGTCCTGCCGTTTGAACCGGTGCCAGTCCTGGAAATCCCAGGGATTGGAAATCTTCCTGCTGTGACTGTTTGTGATGAGCTGAAAATTCAGAGCCAGAATGACCGTGAAAAACTTGCAGAAGCAAAGGAGAAATTGTATTTAAGAGGATTTTATGATGGTGTAATGCTAGTGGATGGCTTTAAAGGACAGAAGATCCAACATGTAAAGAAGACTATTCAGAAAAACATGATTGATGCTGGAGATGCGCTTATCTACATGGAGCCAGAAAAACAAGTGATGTCGAGGTCTGCAGATGAGTGCGTCGTGGCCCTGTGTGACCAGTGGTACTTGGATTATGGAGATGAGAACTGGAAGAAACAGACATTTCAGTGCCTGAAGAACATGGAAACATTCTGCGAGGAGAGCAGGAAGAATTTTGAAGCTTCCTTAGACTGGCTACAAGAACATGCTTGCTCAAGAACGTACGGTTTAGGCACTCGTCTGCCTTGGGATGAACAGTGGTTAATCGAATCTCTCTCGGATTCCACAATTTACATGGCGTTCTACACAGTGGCACACCTGCTGCAGGGGGGTGACCTGAATGGGCAGGCGGAGTCTCCACTGGGCATCAGACCCCAGCAGATGACAAAGGACGTTTGGGATTATGTGTTCTTCAAGGATGCTCCATTTCCTAAGACTCAGATCCCAAAGGAGAAGCTGGATCAGTTGAAGCAGGAGTTTGAGTTTTGGTACCCAGTGGACCTTCGAGCCTCTGGCAAGGATCTGATTCCAAATCATCTTTCATATTACATTTACAACCATGTGGCCATGTGGCCAGAGCAAAGTTTGCAGTAG
Peptide sequence:
MPIKACADKLKREIELYGCPPDFPEEEEEEEESSAKPGDIVVRDKAKGKKSKAAAKAGSSKYQWDIMKSLGLSDDDIVKFSEAEHWLDYFPPLAVQDLKTIGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLVKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETANGDIFICTQRAARNMSYQGFTKHNGVVPVVKELMGEEILGASLSAPLTCYKVVYVLPMLTIKEDKGTGVVTSVPSDSPDDLAALRDLKKKQALRTKFGIRDDMVLPFEPVPVLEIPGIGNLPAVTVCDELKIQSQNDREKLAEAKEKLYLRGFYDGVMLVDGFKGQKIQHVKKTIQKNMIDAGDALIYMEPEKQVMSRSADECVVALCDQWYLDYGDENWKKQTFQCLKNMETFCEESRKNFEASLDWLQEHACSRTYGLGTRLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGDLNGQAESPLGIRPQQMTKDVWDYVFFKDAPFPKTQIPKEKLDQLKQEFEFWYPVDLRASGKDLIPNHLSYYIYNHVAMWPEQSLQ
IRESfinder result
IRESfinder score
0.528485865
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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