top of page
mmu_circKif13b_426
Basic information of circular RNA
PanCircBase ID:
mmu_circKif13b_426
mm39_Chr. Position ID:
chr14|64987661|64989545|+
Gene name:
Kif13b
Transcript:
ENSMUST00000100473.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
19,20,21
Exon/intron offsets:
0,1293,1755
Genomic length:
1884
Exon/intron sizes in nt:
14,71,50,129
Flanking exon/introns:
chr14:64986057-64987661|chr14:64989545-64994862
Splice length:
426
Other database IDs:
circAtlas: mmu-Kif13b_0065
Human Ortholog IDs:
Mature spliced Sequence:
CGAGGCTCTCTCCTCAGTGAACCTGCCATCCAGGTGAGAAGAAAGGGCAAAGGAAAGCAGATTTGGTCTTTGGAAAAGCTGGAAAACAGGCTTTTGGATATGAGAGACCTCTACCAGGAGTGGAAGGAGTGTGAAGAAGACAGCCCGGTTAGCCGGTCCTACTTCAAGCGTGCTGACCCATTCTATGATGAGCAGGAAAACCATAGTCTCATCGGTGTGGCCAATGTCTTCCTGGAGACCCTGTTTTATGACGTGAAGTTACAGTATGCTGTGCCGATCATCAACCAGAAGGGAGAGGTGGCAGGCCGGCTGCACGTGGAGGTGATGCGACTCAGTGGGGCTATTGGGGAGAGGATTGCAGGAGGAGACGATCCTACAGAGGTCAGCTCTGAAAAGGAAGCCCAGGAGAACAGGCTGGTCTGCATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGACTCAGTGGGGCTATTGG
60.18
CTTCTCACCTGGATGGCAGG
60.11
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGACTCAGTGGGGCTATTGG
60.18
TGCCCTTTCTTCTCACCTGG
59.6
148
PCR template sequence:
GCGACTCAGTGGGGCTATTGGGGAGAGGATTGCAGGAGGAGACGATCCTACAGAGGTCAGCTCTGAAAAGGAAGCCCAGGAGAACAGGCTGGTCTGCATGCGAGGCTCTCTCCTCAGTGAACCTGCCATCCAGGTGAGAAGAAAGGGCAAAGGAAAGCAGATTTGGTCTTTGGAAAAGCTGGAAAACAGGCTTTTGGATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-207(2), mmu-miR-7b-5p(1), mmu-miR-1199-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTGGTCTGCATGCGAGGCTCTCTCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIF13B_426_ORF_1
1.2799
0.470939943
0.988754731
753
ORF sequence:
ATGAGAGACCTCTACCAGGAGTGGAAGGAGTGTGAAGAAGACAGCCCGGTTAGCCGGTCCTACTTCAAGCGTGCTGACCCATTCTATGATGAGCAGGAAAACCATAGTCTCATCGGTGTGGCCAATGTCTTCCTGGAGACCCTGTTTTATGACGTGAAGTTACAGTATGCTGTGCCGATCATCAACCAGAAGGGAGAGGTGGCAGGCCGGCTGCACGTGGAGGTGATGCGACTCAGTGGGGCTATTGGGGAGAGGATTGCAGGAGGAGACGATCCTACAGAGGTCAGCTCTGAAAAGGAAGCCCAGGAGAACAGGCTGGTCTGCATGCGAGGCTCTCTCCTCAGTGAACCTGCCATCCAGGTGAGAAGAAAGGGCAAAGGAAAGCAGATTTGGTCTTTGGAAAAGCTGGAAAACAGGCTTTTGGATATGAGAGACCTCTACCAGGAGTGGAAGGAGTGTGAAGAAGACAGCCCGGTTAGCCGGTCCTACTTCAAGCGTGCTGACCCATTCTATGATGAGCAGGAAAACCATAGTCTCATCGGTGTGGCCAATGTCTTCCTGGAGACCCTGTTTTATGACGTGAAGTTACAGTATGCTGTGCCGATCATCAACCAGAAGGGAGAGGTGGCAGGCCGGCTGCACGTGGAGGTGATGCGACTCAGTGGGGCTATTGGGGAGAGGATTGCAGGAGGAGACGATCCTACAGAGGTCAGCTCTGAAAAGGAAGCCCAGGAGAACAGGCTGGTCTGCATG
Peptide sequence:
MRDLYQEWKECEEDSPVSRSYFKRADPFYDEQENHSLIGVANVFLETLFYDVKLQYAVPIINQKGEVAGRLHVEVMRLSGAIGERIAGGDDPTEVSSEKEAQENRLVCMRGSLLSEPAIQVRRKGKGKQIWSLEKLENRLLDMRDLYQEWKECEEDSPVSRSYFKRADPFYDEQENHSLIGVANVFLETLFYDVKLQYAVPIINQKGEVAGRLHVEVMRLSGAIGERIAGGDDPTEVSSEKEAQENRLVCM
IRESfinder result
IRESfinder score
0.307002259
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page