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mmu_circIft74_897
Basic information of circular RNA
PanCircBase ID:
mmu_circIft74_897
mm39_Chr. Position ID:
chr4|94543065|94575173|+
Gene name:
Ift74
Transcript:
ENSMUST00000030311.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
10,11,12,13,14,15,16,17,18
Exon/intron offsets:
0,3678,6119,7758,23436,24351,25105,30793,31982
Genomic length:
32108
Exon/intron sizes in nt:
63,144,41,80,54,98,127,164,126
Flanking exon/introns:
chr4:94541302-94543065|chr4:94575173-94579956
Splice length:
897
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAATTAGATACACTTCAGCAACAGCTAGATTCTCTGAACATGAAAAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTATTGCTGTATGAAAAACTTTATGAGTTAGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATGGGATCGCCAATGGAGGAAAGGGAGAGATTGCTTAAGCAGGTTAAAGAAGATAATCAGGAAATAGCCAGCATGGAGAGACAGTTAACAGATATAAAAGAAAAAATAAACCAATTTAGTGAAGAAATTAGACAACTTGACATGGATTTAGAGGAGCACCAAGGTGAAATGAATCAAAAATACAAGGAGCTTAAAAAAAGGGAGGAAAATATGGATGCTTTTATAGAGACTTTTGAGGAAACGAAGAATCAGGAACTAGAGCGGAAGGCACAGATAGAAGCCAGCATCATCACACTGTTAGAGCATTGCAGTCGGAATATAAATCGTATGAAGCAGATATCCTCCATCACCAACCAAGAGCTGAAGATGATGCAGGATGACCTCAGCTTTAAATCAACTGAGATGCAGAAGTCACAGACAACAGCTAGGAATTTGACCTCAGACAGTCAGCGTCTGCAGCTGGATCTACAGAAAATGGAACTTCTGGAAAGTAAGATGACAGAAGAGCAACAGTCTCTGAAAAACAAAATTAAGCAAATGACTGCTGACCTGGAGACATACAGTGACCTGGCAGCTTTAAAATCATCAGCTGAAGAAAAGAAGAAGAAATTGCATCAAGAAAGAACAGTATTGTCAACTCACAGAAATGCCTTTAAGAAAATAATGGAGAAGCTGACCTCAGATTATGATACCCTTAAAAGGCAACTTCAAGACAATGAGACACATGCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGAAGCTGACCTCAGAT
57.76
ACTTGGGAGTGTGCTATTTCAGT
59.93
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGGAGAAGCTGACCTCAGA
57.76
ACTTGGGAGTGTGCTATTTCAGT
59.93
152
PCR template sequence:
GTCAACTCACAGAAATGCCTTTAAGAAAATAATGGAGAAGCTGACCTCAGATTATGATACCCTTAAAAGGCAACTTCAAGACAATGAGACACATGCTCAGGAATTAGATACACTTCAGCAACAGCTAGATTCTCTGAACATGAAAAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-122-5p(1), mmu-miR-211-5p(1), mmu-miR-320-3p(1), mmu-miR-136-5p(1), mmu-miR-22-3p(1), mmu-miR-297a-5p(1), mmu-miR-384-3p(1), mmu-miR-3105-3p(1), mmu-miR-574-5p(1), mmu-miR-344-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GACACATGCTCAGGAATTAGATACAC
siRNA sets
TAATTCCTGAGCATGTGTC, TATCTAATTCCTGAGCATG, GTGTATCTAATTCCTGAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCIFT74_897_ORF_1
1.1376
0.184890609
0.999964683
1755
ORF sequence:
ATGAAAAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTATTGCTGTATGAAAAACTTTATGAGTTAGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATGGGATCGCCAATGGAGGAAAGGGAGAGATTGCTTAAGCAGGTTAAAGAAGATAATCAGGAAATAGCCAGCATGGAGAGACAGTTAACAGATATAAAAGAAAAAATAAACCAATTTAGTGAAGAAATTAGACAACTTGACATGGATTTAGAGGAGCACCAAGGTGAAATGAATCAAAAATACAAGGAGCTTAAAAAAAGGGAGGAAAATATGGATGCTTTTATAGAGACTTTTGAGGAAACGAAGAATCAGGAACTAGAGCGGAAGGCACAGATAGAAGCCAGCATCATCACACTGTTAGAGCATTGCAGTCGGAATATAAATCGTATGAAGCAGATATCCTCCATCACCAACCAAGAGCTGAAGATGATGCAGGATGACCTCAGCTTTAAATCAACTGAGATGCAGAAGTCACAGACAACAGCTAGGAATTTGACCTCAGACAGTCAGCGTCTGCAGCTGGATCTACAGAAAATGGAACTTCTGGAAAGTAAGATGACAGAAGAGCAACAGTCTCTGAAAAACAAAATTAAGCAAATGACTGCTGACCTGGAGACATACAGTGACCTGGCAGCTTTAAAATCATCAGCTGAAGAAAAGAAGAAGAAATTGCATCAAGAAAGAACAGTATTGTCAACTCACAGAAATGCCTTTAAGAAAATAATGGAGAAGCTGACCTCAGATTATGATACCCTTAAAAGGCAACTTCAAGACAATGAGACACATGCTCAGGAATTAGATACACTTCAGCAACAGCTAGATTCTCTGAACATGAAAAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTATTGCTGTATGAAAAACTTTATGAGTTAGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATGGGATCGCCAATGGAGGAAAGGGAGAGATTGCTTAAGCAGGTTAAAGAAGATAATCAGGAAATAGCCAGCATGGAGAGACAGTTAACAGATATAAAAGAAAAAATAAACCAATTTAGTGAAGAAATTAGACAACTTGACATGGATTTAGAGGAGCACCAAGGTGAAATGAATCAAAAATACAAGGAGCTTAAAAAAAGGGAGGAAAATATGGATGCTTTTATAGAGACTTTTGAGGAAACGAAGAATCAGGAACTAGAGCGGAAGGCACAGATAGAAGCCAGCATCATCACACTGTTAGAGCATTGCAGTCGGAATATAAATCGTATGAAGCAGATATCCTCCATCACCAACCAAGAGCTGAAGATGATGCAGGATGACCTCAGCTTTAAATCAACTGAGATGCAGAAGTCACAGACAACAGCTAGGAATTTGACCTCAGACAGTCAGCGTCTGCAGCTGGATCTACAGAAAATGGAACTTCTGGAAAGTAAGATGACAGAAGAGCAACAGTCTCTGAAAAACAAAATTAAGCAAATGACTGCTGACCTGGAGACATACAGTGACCTGGCAGCTTTAAAATCATCAGCTGAAGAAAAGAAGAAGAAATTGCATCAAGAAAGAACAGTATTGTCAACTCACAGAAATGCCTTTAAGAAAATAATGGAGAAGCTGACCTCAGATTATGATACCCTTAAAAGGCAACTTCAAGACAATGAGACACATGCTCAG
Peptide sequence:
MKKESLETEIAHSQVKQEAVLLYEKLYELESHRDQMIAEDKSMGSPMEERERLLKQVKEDNQEIASMERQLTDIKEKINQFSEEIRQLDMDLEEHQGEMNQKYKELKKREENMDAFIETFEETKNQELERKAQIEASIITLLEHCSRNINRMKQISSITNQELKMMQDDLSFKSTEMQKSQTTARNLTSDSQRLQLDLQKMELLESKMTEEQQSLKNKIKQMTADLETYSDLAALKSSAEEKKKKLHQERTVLSTHRNAFKKIMEKLTSDYDTLKRQLQDNETHAQELDTLQQQLDSLNMKKESLETEIAHSQVKQEAVLLYEKLYELESHRDQMIAEDKSMGSPMEERERLLKQVKEDNQEIASMERQLTDIKEKINQFSEEIRQLDMDLEEHQGEMNQKYKELKKREENMDAFIETFEETKNQELERKAQIEASIITLLEHCSRNINRMKQISSITNQELKMMQDDLSFKSTEMQKSQTTARNLTSDSQRLQLDLQKMELLESKMTEEQQSLKNKIKQMTADLETYSDLAALKSSAEEKKKKLHQERTVLSTHRNAFKKIMEKLTSDYDTLKRQLQDNETHAQ
IRESfinder result
IRESfinder score
0.538425908
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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