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mmu_circIbtk_2920
Basic information of circular RNA
PanCircBase ID:
mmu_circIbtk_2920
mm39_Chr. Position ID:
chr9|85588496|85617152|-
Gene name:
Ibtk
Transcript:
ENSMUST00000039213.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
20
Exon/intron indexes:
23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,1580,3932,7153,7744,8855,11107,11917,12451,12569,13419,14300,15831,17603,20166,21962,22232,24640,26187,28531
Genomic length:
28656
Exon/intron sizes in nt:
16,11,49,11,31,22,61,10,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr9:85585796-85588496|chr9:85617152-85619520
Splice length:
2920
Other database IDs:
circAtlas: hsa-IBTK_0085, CIRCpedia: HSA_CIRCpedia_55610
Human Ortholog IDs:
Mature spliced Sequence:
ATCCCACAGAAGTCTATACTTGGGGAGATAACACAAACTTCACTCTGGGCCACGGAAGCCAGAATAGCAAACATCACCCCGAGCTGCTGGACCTGTTCTCCAGGAGTGGGGTTTATGTCAAGCAGGTGGTGCTTTGCAAATTTCATTCGGTGTTTCTGTCCCAGAAAGGGCAGGTTTACACCTGTGGCCATGGTCGCGGAGGACGGTTAGGCCATGGAGATGAACAGACATGCTTGGTTCCCAGGCTTGTGGAAGGACTGTCTGGTCACAATTGTTCGCAAGTGGCAGCTGCTAAGGATCACACTGTTGTGTTAACTGATGATGGATGTGTCTATACATTTGGTCTCAACATGTTTCACCAATTAGGAATTATTCCTCCACCTGCTAGTTGTAATGTACCCAGACAGATACAGGCAAAATATCTGAAAGGAAGGACTATCATTGGAGTAGCAGCGGGCAGGTTTCATACGGTGCTGTGGACTCGAGAAGCCGTTTACACACTGGGACTGAATGGTGGACAGTTGGGTCACTTACTGGATCCCAATGGTGAAAAGTGTGTCACAACCCCTCGCCAAGTCTCTGCCCTTCATCACAAGGATATCGCTGTGTCTTTGGTAGCTGCAAGTGATGGTGCCACAGTCTGTGTCACCACAAGGGGAGACATTTACTTACTTGCAGACTATCAGTGCAAGAAGATGGCAACTAAACAACTGAACTTGAAAAAGGTTCTTGTCTCTGGAGGTTGTATGGAATACAAGGTTGATCCTGAACATTTGACAGAAAATGGAGGTCAGAAAATATGTGTCCTCGCTATGGATGGAGCTGGAAGGGTGTTTTGCTGGAGATCAATCAGCAGTTCTCTGAAGCAGTGCCGATGGGCCTATCCTCGCCAGGTGTCAATCTCCGATATTGCTTTAAATAGAAATGAAATTCTGTTTGTCACACAGGATGGGGAAGGATTTAAAGGGAAATGGTTTGAAGACAAAAGAAAGAATTCTGAAAAGAAAGCAGACATTTTACCAAACCTCCATCATTCCTCATCAGATGTGTCTTGTGTCCCTGATACAAATAGTGTGTATGAAAGAATTCGACTTGAGAAACTTCCTTTTGCCCATAGAGCTGTTAGTGTCAGCACAGATCCAAGTGGGTGCAACTTTGCAATCCTACAGTCAGACCCTAAAACAAGCCTTTATGAGATTCCAGTTGTGTCTTCATCATCTTTTTTTGAAGAATTTGGCAAGCTCCTGAGGGAGACAGATGAAATGGACAGCTTCCACGATGTGACCTTTCAAGTTGGCAATAGACACTTCCCCGCACATAAATACATTTTGGCCGTGCGCTCTGACTTCTTCCAGAAGTTGTTTCTTTCAGATGGCTCTTCTTTAGAGCTGACGGATGTTTACCAGAAAGACGAAGATGCTGCTGGCTGCCATCTCTTTGTGGTGGAGAAAGTCCATCCTGACTTGTTCGAATACCTCTTACAGTTCATGTACACAGATACCTGTGACTTGTTAACTCATGGCTTCAAACCAAGAATGATCGTGAAGAGAAAGGCAGAAGACTGTGAGGGCTCCCCAGATTCTCATCTGCACACTGTGAATTGCCATGTGGATGATAAACAGAAGTCAGCATTTGAGGTGTACAGAAGCAACCAGGCCCACACACTTAGTGAACGGCAGAAGAGCAAACCCAAATCTTCTAAGAAGGGAAAAGGTGTTGGGGACGACGACCCTGTAAGGATGTTGCAAAGTGTTGCTAAGAAGTTTGGCCTCAGTAATTTGAGTAGCAGGTTAGAAGGAGTCAGATTGGAAAATGAAAAAATTAATGTTATTGCGAAGAAAACTGGCAATAAACTGAAGCTAAGTCAGAAAAAATGTTCGTTTCTGTATGATGTCACCATGAAGTCTGTGGATGGAAAGGAGTTCTCCTGCCATAAGTGTGTGCTCTGTGCGAGACTTGAGTATTTCCACAGCATGCTTAGCAGATCGTGGATTGAGGCCTCCAGCTGTGCAGCTCTGGAAATGCCAATCCAGTCTGAGATTCTGAAAGTGATTTTGGACTACCTCTACACTGATGAGGCTGTGGTGATAAAAGAGTCTCAAAACGTGGACTTTGTTTGTAGTGTTCTTGTCGTGGCTGATCAGCTTCTCATAACTCGGTTAAAGGAGATTTGTGAAGTAGCATTGACTGAAAACCTTACCCTTAAGAATGCTGCGATGCTGCTGGAGTTTGCAGCCTTGTACAATGCTGGGCAGTTGAAGCTCTCTTGTTTACAGTTCATAGGACTGAATATGGCAGCTTTACTTGAAGCAAGGTCTCTGGATGTTTTAAGTGAGGATGTTTTGAAGGACCTTTCCATATTCTACAGAAAAATGATTCCAGCAATGGAGAGAAGAGTCATTACACCGTATCAAGATGGGCCAGATATTAGCTCTATGCAAGTAGAAGACGGAGAGGTCTTTTTTAAAGAAGAAATAAACATGGAACCAAATTACTCGGAAACTATGTTCAAGAAGGCGAAAACAAGAGCTAAAAAGAAGCCACGGAAGCGCTCAGACAGTTCAGGAGGCTACACCCTTTCAGATGTTATCCAGAGTCCACCGTCTGCAGGATTATTAAAGTCTGCAAAGACCAATTCTGTGGAGTCTCTTCCAGAATTGTTGACCTCAGATTCGGAAGGAAGCTATGCAGGAGTGGCGAGTCCCAGAGACCTCCAGTCGCCCGACTTCACAGCAGGATTCCATTCGGATAAGGTCGAGGGGAAAGCTAAGCCATACGTGAATGGTATACCTCCTCCATGTACTAGGGAAGATGTAAAGCCGTGGGAGAAATCACCAACAACTAAGTCTGCTCCCCAGTTCATCCCCAGTAACAGAGTGGACACTGCAGCCTCTTCCAGTTGGCTTGCCGGCTCTTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTGCTCCCCAGTTCATCC
60.11
TGCTATTCTGGCTTCCGTGG
60.11
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTGCTCCCCAGTTCATCC
60.11
GAGAACAGGTCCAGCAGCTC
60.39
175
PCR template sequence:
CGTGGGAGAAATCACCAACAACTAAGTCTGCTCCCCAGTTCATCCCCAGTAACAGAGTGGACACTGCAGCCTCTTCCAGTTGGCTTGCCGGCTCTTGCAGATCCCACAGAAGTCTATACTTGGGGAGATAACACAAACTTCACTCTGGGCCACGGAAGCCAGAATAGCAAACATCACCCCGAGCTGCTGGACCTGTTCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
33
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(2), mmu-miR-322-5p(2), mmu-miR-15b-5p(2), mmu-miR-136-5p(2), mmu-miR-22-3p(2), mmu-miR-107-3p(2), mmu-miR-16-5p(1), mmu-miR-199a-5p(1), mmu-miR-135a-5p(1), mmu-miR-30e-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-335-5p(1), mmu-miR-29c-3p(1), mmu-miR-146a-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-216a-5p(1), mmu-miR-217-5p(1), mmu-miR-196a-5p(1), mmu-miR-326-3p(1), mmu-miR-30d-5p(1), mmu-miR-30b-5p(1), mmu-miR-758-3p(1), mmu-miR-98-5p(1), mmu-miR-335-3p(1), mmu-miR-452-3p(1), mmu-miR-350-3p(1), mmu-miR-146b-5p(1), mmu-miR-202-3p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCGGCTCTTGCAGATCCCACAGAAGT
siRNA sets
TTCTGTGGGATCTGCAAGA, CTTCTGTGGGATCTGCAAG, CTGTGGGATCTGCAAGAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCIBTK_2920_ORF_1
0.8535
0.101131051
0.999999793
2601
ORF sequence:
ATGTTTCACCAATTAGGAATTATTCCTCCACCTGCTAGTTGTAATGTACCCAGACAGATACAGGCAAAATATCTGAAAGGAAGGACTATCATTGGAGTAGCAGCGGGCAGGTTTCATACGGTGCTGTGGACTCGAGAAGCCGTTTACACACTGGGACTGAATGGTGGACAGTTGGGTCACTTACTGGATCCCAATGGTGAAAAGTGTGTCACAACCCCTCGCCAAGTCTCTGCCCTTCATCACAAGGATATCGCTGTGTCTTTGGTAGCTGCAAGTGATGGTGCCACAGTCTGTGTCACCACAAGGGGAGACATTTACTTACTTGCAGACTATCAGTGCAAGAAGATGGCAACTAAACAACTGAACTTGAAAAAGGTTCTTGTCTCTGGAGGTTGTATGGAATACAAGGTTGATCCTGAACATTTGACAGAAAATGGAGGTCAGAAAATATGTGTCCTCGCTATGGATGGAGCTGGAAGGGTGTTTTGCTGGAGATCAATCAGCAGTTCTCTGAAGCAGTGCCGATGGGCCTATCCTCGCCAGGTGTCAATCTCCGATATTGCTTTAAATAGAAATGAAATTCTGTTTGTCACACAGGATGGGGAAGGATTTAAAGGGAAATGGTTTGAAGACAAAAGAAAGAATTCTGAAAAGAAAGCAGACATTTTACCAAACCTCCATCATTCCTCATCAGATGTGTCTTGTGTCCCTGATACAAATAGTGTGTATGAAAGAATTCGACTTGAGAAACTTCCTTTTGCCCATAGAGCTGTTAGTGTCAGCACAGATCCAAGTGGGTGCAACTTTGCAATCCTACAGTCAGACCCTAAAACAAGCCTTTATGAGATTCCAGTTGTGTCTTCATCATCTTTTTTTGAAGAATTTGGCAAGCTCCTGAGGGAGACAGATGAAATGGACAGCTTCCACGATGTGACCTTTCAAGTTGGCAATAGACACTTCCCCGCACATAAATACATTTTGGCCGTGCGCTCTGACTTCTTCCAGAAGTTGTTTCTTTCAGATGGCTCTTCTTTAGAGCTGACGGATGTTTACCAGAAAGACGAAGATGCTGCTGGCTGCCATCTCTTTGTGGTGGAGAAAGTCCATCCTGACTTGTTCGAATACCTCTTACAGTTCATGTACACAGATACCTGTGACTTGTTAACTCATGGCTTCAAACCAAGAATGATCGTGAAGAGAAAGGCAGAAGACTGTGAGGGCTCCCCAGATTCTCATCTGCACACTGTGAATTGCCATGTGGATGATAAACAGAAGTCAGCATTTGAGGTGTACAGAAGCAACCAGGCCCACACACTTAGTGAACGGCAGAAGAGCAAACCCAAATCTTCTAAGAAGGGAAAAGGTGTTGGGGACGACGACCCTGTAAGGATGTTGCAAAGTGTTGCTAAGAAGTTTGGCCTCAGTAATTTGAGTAGCAGGTTAGAAGGAGTCAGATTGGAAAATGAAAAAATTAATGTTATTGCGAAGAAAACTGGCAATAAACTGAAGCTAAGTCAGAAAAAATGTTCGTTTCTGTATGATGTCACCATGAAGTCTGTGGATGGAAAGGAGTTCTCCTGCCATAAGTGTGTGCTCTGTGCGAGACTTGAGTATTTCCACAGCATGCTTAGCAGATCGTGGATTGAGGCCTCCAGCTGTGCAGCTCTGGAAATGCCAATCCAGTCTGAGATTCTGAAAGTGATTTTGGACTACCTCTACACTGATGAGGCTGTGGTGATAAAAGAGTCTCAAAACGTGGACTTTGTTTGTAGTGTTCTTGTCGTGGCTGATCAGCTTCTCATAACTCGGTTAAAGGAGATTTGTGAAGTAGCATTGACTGAAAACCTTACCCTTAAGAATGCTGCGATGCTGCTGGAGTTTGCAGCCTTGTACAATGCTGGGCAGTTGAAGCTCTCTTGTTTACAGTTCATAGGACTGAATATGGCAGCTTTACTTGAAGCAAGGTCTCTGGATGTTTTAAGTGAGGATGTTTTGAAGGACCTTTCCATATTCTACAGAAAAATGATTCCAGCAATGGAGAGAAGAGTCATTACACCGTATCAAGATGGGCCAGATATTAGCTCTATGCAAGTAGAAGACGGAGAGGTCTTTTTTAAAGAAGAAATAAACATGGAACCAAATTACTCGGAAACTATGTTCAAGAAGGCGAAAACAAGAGCTAAAAAGAAGCCACGGAAGCGCTCAGACAGTTCAGGAGGCTACACCCTTTCAGATGTTATCCAGAGTCCACCGTCTGCAGGATTATTAAAGTCTGCAAAGACCAATTCTGTGGAGTCTCTTCCAGAATTGTTGACCTCAGATTCGGAAGGAAGCTATGCAGGAGTGGCGAGTCCCAGAGACCTCCAGTCGCCCGACTTCACAGCAGGATTCCATTCGGATAAGGTCGAGGGGAAAGCTAAGCCATACGTGAATGGTATACCTCCTCCATGTACTAGGGAAGATGTAAAGCCGTGGGAGAAATCACCAACAACTAAGTCTGCTCCCCAGTTCATCCCCAGTAACAGAGTGGACACTGCAGCCTCTTCCAGTTGGCTTGCCGGCTCTTGCAGATCCCACAGAAGTCTATACTTGGGGAGATAA
Peptide sequence:
MFHQLGIIPPPASCNVPRQIQAKYLKGRTIIGVAAGRFHTVLWTREAVYTLGLNGGQLGHLLDPNGEKCVTTPRQVSALHHKDIAVSLVAASDGATVCVTTRGDIYLLADYQCKKMATKQLNLKKVLVSGGCMEYKVDPEHLTENGGQKICVLAMDGAGRVFCWRSISSSLKQCRWAYPRQVSISDIALNRNEILFVTQDGEGFKGKWFEDKRKNSEKKADILPNLHHSSSDVSCVPDTNSVYERIRLEKLPFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPVVSSSSFFEEFGKLLRETDEMDSFHDVTFQVGNRHFPAHKYILAVRSDFFQKLFLSDGSSLELTDVYQKDEDAAGCHLFVVEKVHPDLFEYLLQFMYTDTCDLLTHGFKPRMIVKRKAEDCEGSPDSHLHTVNCHVDDKQKSAFEVYRSNQAHTLSERQKSKPKSSKKGKGVGDDDPVRMLQSVAKKFGLSNLSSRLEGVRLENEKINVIAKKTGNKLKLSQKKCSFLYDVTMKSVDGKEFSCHKCVLCARLEYFHSMLSRSWIEASSCAALEMPIQSEILKVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKEICEVALTENLTLKNAAMLLEFAALYNAGQLKLSCLQFIGLNMAALLEARSLDVLSEDVLKDLSIFYRKMIPAMERRVITPYQDGPDISSMQVEDGEVFFKEEINMEPNYSETMFKKAKTRAKKKPRKRSDSSGGYTLSDVIQSPPSAGLLKSAKTNSVESLPELLTSDSEGSYAGVASPRDLQSPDFTAGFHSDKVEGKAKPYVNGIPPPCTREDVKPWEKSPTTKSAPQFIPSNRVDTAASSSWLAGSCRSHRSLYLGR
IRESfinder result
IRESfinder score
0.621057185
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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