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mmu_circHuwe1_2279
Basic information of circular RNA
PanCircBase ID:
mmu_circHuwe1_2279
mm39_Chr. Position ID:
chrX|150679558|150693251|+
Gene name:
Huwe1
Transcript:
ENSMUST00000026292.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
15
Exon/intron indexes:
44,45,46,47,48,49,50,51,52,53,54,55,56,57,58
Exon/intron offsets:
0,624,1881,4338,4936,7266,7791,8793,9702,10388,10713,12165,12846,13297,13538
Genomic length:
13693
Exon/intron sizes in nt:
14,66,72,18,21,21,83,10,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chrX:150678084-150679558|chrX:150693251-150695204
Splice length:
2279
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CAGACAAATCTGATCCTAAACCTGGGGGAACGACCCAAGAGGTTGGCCAGCTCCTGCAAGACATGGGTGACGATGTATACCAGCAGTACCGGTCACTTACTCGTCAGAGCAGTGACTTTGATACACAGTCAGGTTTTTCCTTAAATAGTCAGGTTTTTGCTGCAGATGGTGCCCCTGCTGAAACTTCCACAACTGGAACCTCCCAAGGAGAGGGAGCTTCAACTCCAGAGGAGACTCGAGAAGGGAAGAAAGATAAAGAAGGGGACCGTACATCTGAGGAAGGCAAGCAGAAAAGCAAGGGCAGCAAACCTTTAATGCCTACCTCTACTATCCTTCGTCTTCTGGCAGAGTTGGTGAGGTCCTATGTTGGTATTGCTACCCTGATTGCAAACTACAGCTATACCGTGGGCCAGTCTGAGCTGATCAAAGAGGACTGCAGTGTGCTAGCTTTTGTCCTGGACCACCTCCTCCCACACACCCAGAATGCAGAAGACAAGGATACACCTGCCCTGGCACGTCTATTCCTTGCAAGTCTGGCTGCTGCGGGGAGTGGCACAGATGCTCAAGTGGCCCTAGTGAATGAAGTGAAAGCAGCCCTGGGACGGGCGCTTGCTATGGCTGAGAGTACAGAGAAGCACGCCAGACTTCAGGCAGTGATGTGTATCATCAGTACTATCATGGAATCCTGCCCCTCCACCTCCAGCTTCTACAGTAGTGCTACAGCCAAGACTCAGCATAATGGCATGAACAACATCATTCGACTGTTCCTAAAGAAGGGACTAGTCAATGACCTGGCCAGAGTTCCTCACAGCCTAGACCTGTCCAGTCCCAATATGGCCAACACAGTCAATGCTGCTCTAAAACCCTTGGAAACACTTTCCCGGATTGTAAATCAACCCAGTAGCCTTTTTGGCAGCAAGAGTGCTTCTAGCAAGAACAAGTCTGAGCAGGATGCTCAAGGAGCTTCTCAGGACTCCAGCAGCCATCAGCAAGATCCAGGCGAGCCTGGGGAGGCAGAAGTGCAGGAGGAGGATCATGATGTCACTCAGACAGAGGTGGCAGATGGGGATATCATGGATGGGGAGGCTGAAACCGACTCAGTGGTGATTGCCGGGCAGCCTGAGGTGCTCAGTTCACAAGAGATGCAGGTTGAAAATGAACTGGAGGACCTGATAGATGAGTTGCTTGAGAGGGATGGCGGATCTGGGAACAGTACAATTATAGTGAGCAGAAGTGGAGAGGATGAATCACAAGAGGACGTGCTGATGGATGAAGCTCCTTCCAACCTCAGCCAAGCTTCCACCTTGCAGGCCAACCGAGAAGATTCCATGAATATCCTGGACCCTGAGGATGAGGAGGAACATACTCAGGAAGAGGACAGCAGTGGCAGTAACGAGGATGAGGATGATAGTCAGGATGAAGAGGAGGAGGAGGAGGAAGATGAGGAAGATGATCAGGAGGATGATGAAGGTGAAGAAGGAGATGAAGATGATGACGACGATGGTTCTGAGATGGAATTGGATGAGGATTACCCTGATATGAACGCTTCTCCCTTGGTCCGATTTGAGCGCTTTGACCGGGAGGATGATCTCATCATTGAGTTTGACAACATGTTCTCCAGTGCTACAGACATTCCCCCATCCCCAGGAAATATCCCTACCACCCACCCATTGATGGTACGTCATGCAGATCACAGTTCCCTGACACTGGGCAGTGGTTCTTCCACAACTCGTCTTACCCAGGGCATCGGTCGCAGTCAAAGGACCCTCAGACAGCTCACTGCCAATACAGGCCATACCATTCATGTTCACTACCCTGGGAATCGCCAGCCTAACCCTCCTCTTATACTCCAGAGGTTGCTTGGTCCCTCTGCAGCTGCTGACATTCTTCAGCTGAGCAGCAGCCTTCCATTACAAAGCCGAGGCCGGGCCCGCCTCCTAGTGGGCAATGATGACGTCCATATCATCGCCAGGTCTGATGACGAGCTGCTGGATGACTTTTTTCATGACCAGAGTACAGCCACTAGCCAAGCAGGAACCCTGTCCAGCATCCCAACAGCTCTAACCCGATGGACAGAGGAATGCAAAGTTCTTGATGCTGAGAGTATGCATGACTGTGTTTCAGTGGTTAAAGTGCCCATTGTCAATCACCTGGAATTCTTGAGAGATGAGGAGCTAGAAGAGAGAAGGGAGAAACGCAGGAAACAATTGGCTGAGGAAGAAACAAAAATAATAGACAAAGGCAAAGAAGACAAGGAGAACAGAGATCAGAGTGCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACGCAGGAAACAATTGGCTG
59.69
GTCACCCATGTCTTGCAGGA
59.96
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGAGAAACGCAGGAAACA
59.53
GTCACCCATGTCTTGCAGGA
59.96
164
PCR template sequence:
AGAGAGAAGGGAGAAACGCAGGAAACAATTGGCTGAGGAAGAAACAAAAATAATAGACAAAGGCAAAGAAGACAAGGAGAACAGAGATCAGAGTGCACAGCAGACAAATCTGATCCTAAACCTGGGGGAACGACCCAAGAGGTTGGCCAGCTCCTGCAAGACATGGGTGACGATGTATACCAGCAGTACCGGTCACTTAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-199a-5p(1), mmu-miR-103-3p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-205-5p(1), mmu-miR-322-5p(1), mmu-miR-433-3p(1), mmu-miR-431-5p(1), mmu-miR-351-5p(1), mmu-miR-382-3p(1), mmu-miR-125b-1-3p(1), mmu-miR-324-3p(1), mmu-miR-34b-5p(1), mmu-miR-107-3p(1), mmu-miR-705(1), mmu-miR-207(1), mmu-miR-1904(1), mmu-miR-193b-3p(1), mmu-miR-296-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGAGTGCACAGCAGACAAATCTGA
siRNA sets
TTTGTCTGCTGTGCACTCT,TTGTCTGCTGTGCACTCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCHUWE1_2279_ORF_1
1.1772
0.33402087
0.99999976
2361
ORF sequence:
ATGGGTGACGATGTATACCAGCAGTACCGGTCACTTACTCGTCAGAGCAGTGACTTTGATACACAGTCAGGTTTTTCCTTAAATAGTCAGGTTTTTGCTGCAGATGGTGCCCCTGCTGAAACTTCCACAACTGGAACCTCCCAAGGAGAGGGAGCTTCAACTCCAGAGGAGACTCGAGAAGGGAAGAAAGATAAAGAAGGGGACCGTACATCTGAGGAAGGCAAGCAGAAAAGCAAGGGCAGCAAACCTTTAATGCCTACCTCTACTATCCTTCGTCTTCTGGCAGAGTTGGTGAGGTCCTATGTTGGTATTGCTACCCTGATTGCAAACTACAGCTATACCGTGGGCCAGTCTGAGCTGATCAAAGAGGACTGCAGTGTGCTAGCTTTTGTCCTGGACCACCTCCTCCCACACACCCAGAATGCAGAAGACAAGGATACACCTGCCCTGGCACGTCTATTCCTTGCAAGTCTGGCTGCTGCGGGGAGTGGCACAGATGCTCAAGTGGCCCTAGTGAATGAAGTGAAAGCAGCCCTGGGACGGGCGCTTGCTATGGCTGAGAGTACAGAGAAGCACGCCAGACTTCAGGCAGTGATGTGTATCATCAGTACTATCATGGAATCCTGCCCCTCCACCTCCAGCTTCTACAGTAGTGCTACAGCCAAGACTCAGCATAATGGCATGAACAACATCATTCGACTGTTCCTAAAGAAGGGACTAGTCAATGACCTGGCCAGAGTTCCTCACAGCCTAGACCTGTCCAGTCCCAATATGGCCAACACAGTCAATGCTGCTCTAAAACCCTTGGAAACACTTTCCCGGATTGTAAATCAACCCAGTAGCCTTTTTGGCAGCAAGAGTGCTTCTAGCAAGAACAAGTCTGAGCAGGATGCTCAAGGAGCTTCTCAGGACTCCAGCAGCCATCAGCAAGATCCAGGCGAGCCTGGGGAGGCAGAAGTGCAGGAGGAGGATCATGATGTCACTCAGACAGAGGTGGCAGATGGGGATATCATGGATGGGGAGGCTGAAACCGACTCAGTGGTGATTGCCGGGCAGCCTGAGGTGCTCAGTTCACAAGAGATGCAGGTTGAAAATGAACTGGAGGACCTGATAGATGAGTTGCTTGAGAGGGATGGCGGATCTGGGAACAGTACAATTATAGTGAGCAGAAGTGGAGAGGATGAATCACAAGAGGACGTGCTGATGGATGAAGCTCCTTCCAACCTCAGCCAAGCTTCCACCTTGCAGGCCAACCGAGAAGATTCCATGAATATCCTGGACCCTGAGGATGAGGAGGAACATACTCAGGAAGAGGACAGCAGTGGCAGTAACGAGGATGAGGATGATAGTCAGGATGAAGAGGAGGAGGAGGAGGAAGATGAGGAAGATGATCAGGAGGATGATGAAGGTGAAGAAGGAGATGAAGATGATGACGACGATGGTTCTGAGATGGAATTGGATGAGGATTACCCTGATATGAACGCTTCTCCCTTGGTCCGATTTGAGCGCTTTGACCGGGAGGATGATCTCATCATTGAGTTTGACAACATGTTCTCCAGTGCTACAGACATTCCCCCATCCCCAGGAAATATCCCTACCACCCACCCATTGATGGTACGTCATGCAGATCACAGTTCCCTGACACTGGGCAGTGGTTCTTCCACAACTCGTCTTACCCAGGGCATCGGTCGCAGTCAAAGGACCCTCAGACAGCTCACTGCCAATACAGGCCATACCATTCATGTTCACTACCCTGGGAATCGCCAGCCTAACCCTCCTCTTATACTCCAGAGGTTGCTTGGTCCCTCTGCAGCTGCTGACATTCTTCAGCTGAGCAGCAGCCTTCCATTACAAAGCCGAGGCCGGGCCCGCCTCCTAGTGGGCAATGATGACGTCCATATCATCGCCAGGTCTGATGACGAGCTGCTGGATGACTTTTTTCATGACCAGAGTACAGCCACTAGCCAAGCAGGAACCCTGTCCAGCATCCCAACAGCTCTAACCCGATGGACAGAGGAATGCAAAGTTCTTGATGCTGAGAGTATGCATGACTGTGTTTCAGTGGTTAAAGTGCCCATTGTCAATCACCTGGAATTCTTGAGAGATGAGGAGCTAGAAGAGAGAAGGGAGAAACGCAGGAAACAATTGGCTGAGGAAGAAACAAAAATAATAGACAAAGGCAAAGAAGACAAGGAGAACAGAGATCAGAGTGCACAGCAGACAAATCTGATCCTAAACCTGGGGGAACGACCCAAGAGGTTGGCCAGCTCCTGCAAGACATGGGTGACGATGTATACCAGCAGTACCGGTCACTTACTCGTCAGAGCAGTGACTTTGATACACAGTCAGGTTTTTCCTTAA
Peptide sequence:
MGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEGASTPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSHQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQDEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVRHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQSAQQTNLILNLGERPKRLASSCKTWVTMYTSSTGHLLVRAVTLIHSQVFP
IRESfinder result
IRESfinder score
0.382573031
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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