top of page
mmu_circGusb_1169
Basic information of circular RNA
PanCircBase ID:
mmu_circGusb_1169
mm39_Chr. Position ID:
chr5|130026620|130029783|-
Gene name:
Gusb
Transcript:
ENSMUST00000026613.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
8,7,6,5,4,3,2
Exon/intron offsets:
0,237,630,1561,2147,2581,2977
Genomic length:
3163
Exon/intron sizes in nt:
14,71,79,14,41,88,14,30,00,000
Flanking exon/introns:
chr5:130024372-130026620|chr5:130029783-130031447
Splice length:
1169
Other database IDs:
circAtlas: mmu-Gusb_0003
circBase: hsa_circ_0006850, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_58505
Human Ortholog IDs:
Mature spliced Sequence:
TCGGGCCCAGTCTTGGACATGCCTGTCCCTTCTAGCTTCAATGACATCACCCAAGAAGCAGCCCTTCGGGACTTTATTGGCTGGGTGTGGTATGAACGGGAAGCAATCCTGCCACGGCGATGGACCCAAGATACCGACATGAGAGTGGTGTTGAGGATCAACAGTGCCCATTATTATGCAGTTGTGTGGGTGAATGGGATTCATGTGGTGGAACATGAGGGAGGTCACCTCCCCTTTGAGGCTGACATTAGCAAGCTGGTCCAGAGTGGGCCCCTGACCACCTGCCGGATCACGATTGCCATCAACAACACACTGACCCCTCATACCCTTCCGCCGGGGACCATCGTCTACAAGACTGACACCTCCATGTATCCCAAGGGTTACTTTGTCCAGGACACAAGCTTTGACTTCTTCAACTATGCGGGACTGCATCGATCTGTGGTCCTCTATACCACCCCTACCACTTACATCGATGATATCACTGTGATCACTAATGTGGAGCAAGACATCGGGCTGGTGACCTACTGGATTTCTGTGCAGGGCAGTGAACATTTCCAGCTAGAAGTGCAACTTTTGGATGAGGGTGGCAAAGTCGTGGCCCATGGGACAGGGAACCAGGGTCAACTTCAGGTTCCCAGTGCCAACCTCTGGTGGCCTTACCTGATGCATGAGCATCCAGCCTACATGTACTCCTTGGAGGTGAAGGTGACAACAACTGAGTCTGTGACTGACTACTACACCCTTCCTATCGGGATTCGAACAGTGGCTGTCACAAAGAGCAAGTTCCTCATAAACGGGAAGCCCTTCTATTTCCAAGGGGTCAATAAGCACGAGGATTCAGATATCCGAGGGAAAGGCTTCGACTGGCCGCTGCTGGTAAAGGATTTCAACCTGCTCCGTTGGCTCGGGGCAAATTCCTTTCGTACCAGCCACTATCCCTACTCAGAGGAGGTACTTCAGCTCTGTGACCGATACGGGATTGTGGTCATCGATGAGTGTCCCGGTGTGGGCATTGTGCTACCTCAGAGTTTTGGCAACGAGTCACTTCGGCACCACCTAGAGGTGATGGAGGAGCTGGTTCGCCGGGACAAAAATCACCCTGCGGTTGTGATGTGGTCTGTGGCCAATGAGCCTTCCTCTGCTCTGAAACCCGCCGCATATTACTTTAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGTTCGCCGGGACAAAAATC
60.11
ACCCAGCCAATAAAGTCCCG
60.03
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAATCACCCTGCGGTTGTGA
60.18
ACCCAGCCAATAAAGTCCCG
60.03
164
PCR template sequence:
AGGAGCTGGTTCGCCGGGACAAAAATCACCCTGCGGTTGTGATGTGGTCTGTGGCCAATGAGCCTTCCTCTGCTCTGAAACCCGCCGCATATTACTTTAATCGGGCCCAGTCTTGGACATGCCTGTCCCTTCTAGCTTCAATGACATCACCCAAGAAGCAGCCCTTCGGGACTTTATTGGCTGGGTGTGGTATGAACGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-29a-3p(1), mmu-miR-298-5p(1), mmu-miR-217-5p(1), mmu-miR-339-5p(1), mmu-let-7d-5p(1), mmu-miR-382-3p(1), mmu-miR-127-5p(1), mmu-miR-96-5p(1), mmu-miR-98-5p(1), mmu-miR-5099(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
CATATTACTTTAATCGGGCCCAGTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGUSB_1169_ORF_1
1.119
0.325335193
0.999415436
1293
ORF sequence:
ATGCCTGTCCCTTCTAGCTTCAATGACATCACCCAAGAAGCAGCCCTTCGGGACTTTATTGGCTGGGTGTGGTATGAACGGGAAGCAATCCTGCCACGGCGATGGACCCAAGATACCGACATGAGAGTGGTGTTGAGGATCAACAGTGCCCATTATTATGCAGTTGTGTGGGTGAATGGGATTCATGTGGTGGAACATGAGGGAGGTCACCTCCCCTTTGAGGCTGACATTAGCAAGCTGGTCCAGAGTGGGCCCCTGACCACCTGCCGGATCACGATTGCCATCAACAACACACTGACCCCTCATACCCTTCCGCCGGGGACCATCGTCTACAAGACTGACACCTCCATGTATCCCAAGGGTTACTTTGTCCAGGACACAAGCTTTGACTTCTTCAACTATGCGGGACTGCATCGATCTGTGGTCCTCTATACCACCCCTACCACTTACATCGATGATATCACTGTGATCACTAATGTGGAGCAAGACATCGGGCTGGTGACCTACTGGATTTCTGTGCAGGGCAGTGAACATTTCCAGCTAGAAGTGCAACTTTTGGATGAGGGTGGCAAAGTCGTGGCCCATGGGACAGGGAACCAGGGTCAACTTCAGGTTCCCAGTGCCAACCTCTGGTGGCCTTACCTGATGCATGAGCATCCAGCCTACATGTACTCCTTGGAGGTGAAGGTGACAACAACTGAGTCTGTGACTGACTACTACACCCTTCCTATCGGGATTCGAACAGTGGCTGTCACAAAGAGCAAGTTCCTCATAAACGGGAAGCCCTTCTATTTCCAAGGGGTCAATAAGCACGAGGATTCAGATATCCGAGGGAAAGGCTTCGACTGGCCGCTGCTGGTAAAGGATTTCAACCTGCTCCGTTGGCTCGGGGCAAATTCCTTTCGTACCAGCCACTATCCCTACTCAGAGGAGGTACTTCAGCTCTGTGACCGATACGGGATTGTGGTCATCGATGAGTGTCCCGGTGTGGGCATTGTGCTACCTCAGAGTTTTGGCAACGAGTCACTTCGGCACCACCTAGAGGTGATGGAGGAGCTGGTTCGCCGGGACAAAAATCACCCTGCGGTTGTGATGTGGTCTGTGGCCAATGAGCCTTCCTCTGCTCTGAAACCCGCCGCATATTACTTTAATCGGGCCCAGTCTTGGACATGCCTGTCCCTTCTAGCTTCAATGACATCACCCAAGAAGCAGCCCTTCGGGACTTTATTGGCTGGGTGTGGTATGAACGGGAAGCAATCCTGCCACGGCGATGGACCCAAGATACCGACATGA
Peptide sequence:
MPVPSSFNDITQEAALRDFIGWVWYEREAILPRRWTQDTDMRVVLRINSAHYYAVVWVNGIHVVEHEGGHLPFEADISKLVQSGPLTTCRITIAINNTLTPHTLPPGTIVYKTDTSMYPKGYFVQDTSFDFFNYAGLHRSVVLYTTPTTYIDDITVITNVEQDIGLVTYWISVQGSEHFQLEVQLLDEGGKVVAHGTGNQGQLQVPSANLWWPYLMHEHPAYMYSLEVKVTTTESVTDYYTLPIGIRTVAVTKSKFLINGKPFYFQGVNKHEDSDIRGKGFDWPLLVKDFNLLRWLGANSFRTSHYPYSEEVLQLCDRYGIVVIDECPGVGIVLPQSFGNESLRHHLEVMEELVRRDKNHPAVVMWSVANEPSSALKPAAYYFNRAQSWTCLSLLASMTSPKKQPFGTLLAGCGMNGKQSCHGDGPKIPT
IRESfinder result
IRESfinder score
0.580620494
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page