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mmu_circGtf2h2_362
Basic information of circular RNA
PanCircBase ID:
mmu_circGtf2h2_362
mm39_Chr. Position ID:
chr13|100605422|100613718|-
Gene name:
Gtf2h2
Transcript:
ENSMUST00000145266.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
16,15,14,13
Exon/intron offsets:
0,1964,7555,8189
Genomic length:
8296
Exon/intron sizes in nt:
112,40,103,107
Flanking exon/introns:
chr13:100598470-100605422|chr13:100613718-100615698
Splice length:
362
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCATTTGGATAACAACAGCACTGAGCCAGGGCTTACACTGGGAGGCTACTTCTGCCCACAGTGCCGAGCAAAGTACTGCGAGCTTCCTGTTGAATGTAAAATATGTGGTCTTACTTTGGTGTCTGCACCTCATTTGGCAAGATCTTACCATCATTTATTTCCTTTGGATGCTTTTCAAGAAATTTCCCTAGAAGAATATAAAGGAGAAAGGTTTTGTTATGGATGTCAGGGGGAATTGAAAGACCAACATGTCTATGTTTGCACAGTGTGCCAAAATGTTTTTTGTGTGGACTGTGATGTCTTTGTTCATGACTCTCTCCATTGTTGTCCTGGCTGTATTCATAAGATCCCAACTCCTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCTCCATTGTTGTCCTGGC
60
TTCAACAGGAAGCTCGCAGT
59.89
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTCCATTGTTGTCCTGGCT
60
TTCAACAGGAAGCTCGCAGT
59.89
142
PCR template sequence:
ACAGTGTGCCAAAATGTTTTTTGTGTGGACTGTGATGTCTTTGTTCATGACTCTCTCCATTGTTGTCCTGGCTGTATTCATAAGATCCCAACTCCTTCAGGCATTTGGATAACAACAGCACTGAGCCAGGGCTTACACTGGGAGGCTACTTCTGCCCACAGTGCCGAGCAAAGTACTGCGAGCTTCCTGTTGAATGTAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-181a-5p(1), mmu-miR-19a-3p(1), mmu-miR-149-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCAACTCCTTCAGGCATTTGGATAAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGTF2H2_362_ORF_1
0.9646
-0.249719316
0.027173959
126
ORF sequence:
ATGTCTATGTTTGCACAGTGTGCCAAAATGTTTTTTGTGTGGACTGTGATGTCTTTGTTCATGACTCTCTCCATTGTTGTCCTGGCTGTATTCATAAGATCCCAACTCCTTCAGGCATTTGGATAA
Peptide sequence:
MSMFAQCAKMFFVWTVMSLFMTLSIVVLAVFIRSQLLQAFG
IRESfinder result
IRESfinder score
0.732439797
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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