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mmu_circGpr158_851
Basic information of circular RNA
PanCircBase ID:
mmu_circGpr158_851
mm39_Chr. Position ID:
chr2|21404131|21788113|+
Gene name:
Gpr158
Transcript:
ENSMUST00000055946.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
2,3,4,5,6,7
Exon/intron offsets:
0,153978,177502,249636,347355,383743
Genomic length:
383982
Exon/intron sizes in nt:
10,61,03,22,46,91,10,200
Flanking exon/introns:
chr2:21373968-21404131|chr2:21788113-21815361
Splice length:
851
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGGTGTGATGAAAGTTGATATAAGCCTTCAGAAAGTGGACATTGACCAATGTTCGAGTGATGGCTGGTTTTCAGGAACTCACAAATGCCACCTTAACAACTCAGAGTGTATGCCAATCAAAGGCCTGGGATTCGTGCTTGGAGCCTATCAGTGCATTTGCAAAGCAGGGTTCTATCATCCCCGTGTCTTCTCAGTGAACAACTTCCAGAGAAGGGGTCCAGATCACCATTTTTCAGGAAGCACAAAGGATGTGTCAGAAGAAACCCACGTCTGCCTGCCCTGCAGAGAAGGCTGCCCCTTCTGTGCTGATGATCGTCCATGCTTTGTCCAGGAGGATAAATACCTGCGGCTCGCTATTATCTCTTTCCAAGCCCTGTGTATGCTGCTGGACTTTGTCAGCATGCTGGTGGTCTACCATTTTCGCAAAGCAAAGAGCATCAGGGCATCTGGGCTCATCCTGTTGGAAACAATATTGTTTGGTTCTCTGCTCCTATACTTTCCGGTCGTTATTCTGTACTTTGAGCCAAGTACATTTCGTTGTATTCTCCTAAGGTGGGCTCGTCTTCTTGGTTTTGCTACTGTTTATGGAACTGTGACCCTCAAGCTTCACAGGGTTTTGAAGGTGTTCCTTTCACGAACAGCACAAAGAATCCCATACATGACTGGTGGTCGGGTCATGAGGATGCTGGCAGTAATAGTCTTGGTTGTGTTTTGGTTTCTTGTTGGCTGGACTTCTTCTATGTGCCAGAATTTGGAGCGGGATATTTTGCTTGTTGGCCAAGGGCAAACATCTGATCACCTCACCTTCAATATGTGCCTCATTGACCGCTGGGATTACATGACAGCAGTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGCAAACATCTGATCACCT
59.36
AACCAGCCATCACTCGAACA
59.6
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGCGGGATATTTTGCTTG
58.98
AACCAGCCATCACTCGAACA
59.6
168
PCR template sequence:
TTGGAGCGGGATATTTTGCTTGTTGGCCAAGGGCAAACATCTGATCACCTCACCTTCAATATGTGCCTCATTGACCGCTGGGATTACATGACAGCAGTTGGGGTGTGATGAAAGTTGATATAAGCCTTCAGAAAGTGGACATTGACCAATGTTCGAGTGATGGCTGGTTTTCAGGAACTCACAAATGCCACCTTAACAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-103-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-127-5p(1), mmu-miR-382-5p(1), mmu-miR-107-3p(1), mmu-miR-383-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGACAGCAGTTGGGGTGTGATGAAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGPR158_851_ORF_1
0.7739
0.029564685
0.918748526
852
ORF sequence:
ATGAAAGTTGATATAAGCCTTCAGAAAGTGGACATTGACCAATGTTCGAGTGATGGCTGGTTTTCAGGAACTCACAAATGCCACCTTAACAACTCAGAGTGTATGCCAATCAAAGGCCTGGGATTCGTGCTTGGAGCCTATCAGTGCATTTGCAAAGCAGGGTTCTATCATCCCCGTGTCTTCTCAGTGAACAACTTCCAGAGAAGGGGTCCAGATCACCATTTTTCAGGAAGCACAAAGGATGTGTCAGAAGAAACCCACGTCTGCCTGCCCTGCAGAGAAGGCTGCCCCTTCTGTGCTGATGATCGTCCATGCTTTGTCCAGGAGGATAAATACCTGCGGCTCGCTATTATCTCTTTCCAAGCCCTGTGTATGCTGCTGGACTTTGTCAGCATGCTGGTGGTCTACCATTTTCGCAAAGCAAAGAGCATCAGGGCATCTGGGCTCATCCTGTTGGAAACAATATTGTTTGGTTCTCTGCTCCTATACTTTCCGGTCGTTATTCTGTACTTTGAGCCAAGTACATTTCGTTGTATTCTCCTAAGGTGGGCTCGTCTTCTTGGTTTTGCTACTGTTTATGGAACTGTGACCCTCAAGCTTCACAGGGTTTTGAAGGTGTTCCTTTCACGAACAGCACAAAGAATCCCATACATGACTGGTGGTCGGGTCATGAGGATGCTGGCAGTAATAGTCTTGGTTGTGTTTTGGTTTCTTGTTGGCTGGACTTCTTCTATGTGCCAGAATTTGGAGCGGGATATTTTGCTTGTTGGCCAAGGGCAAACATCTGATCACCTCACCTTCAATATGTGCCTCATTGACCGCTGGGATTACATGACAGCAGTTGGGGTGTGA
Peptide sequence:
MKVDISLQKVDIDQCSSDGWFSGTHKCHLNNSECMPIKGLGFVLGAYQCICKAGFYHPRVFSVNNFQRRGPDHHFSGSTKDVSEETHVCLPCREGCPFCADDRPCFVQEDKYLRLAIISFQALCMLLDFVSMLVVYHFRKAKSIRASGLILLETILFGSLLLYFPVVILYFEPSTFRCILLRWARLLGFATVYGTVTLKLHRVLKVFLSRTAQRIPYMTGGRVMRMLAVIVLVVFWFLVGWTSSMCQNLERDILLVGQGQTSDHLTFNMCLIDRWDYMTAVGV
IRESfinder result
IRESfinder score
0.643025922
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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