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mmu_circGlul_341
Basic information of circular RNA
PanCircBase ID:
mmu_circGlul_341
mm39_Chr. Position ID:
chr1|153778679|153780444|+
Gene name:
Glul
Transcript:
ENSMUST00000086199.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,1603
Genomic length:
1765
Exon/intron sizes in nt:
1,79,162
Flanking exon/introns:
chr1:153775805-153778679|chr1:153780444-153782094
Splice length:
341
Other database IDs:
CIRCpedia: MMU_CIRCpedia_10493, circAtlas: mmu-Glul_0003
Human Ortholog IDs:
Mature spliced Sequence:
AACACCTTCCACCATGGCCACCTCAGCAAGTTCCCACTTGAACAAAGGCATCAAGCAAATGTACATGTCCCTGCCCCAGGGTGAGAAAGTCCAAGCCATGTATATCTGGGTTGATGGTACCGGAGAAGGACTGCGCTGCAAGACCCGTACCCTGGACTGTGAGCCCAAGTGTGTGGAAGAGTTACCTGAGTGGAACTTTGATGGCTCTAGTACCTTTCAGTCTGAAGGCTCCAACAGCGACATGTACCTCCATCCTGTTGCCATGTTTCGAGACCCCTTCCGCAAAGACCCCAACAAGCTGGTGCTATGTGAAGTTTTCAAGTATAACCGGAAACCTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAAAGACCCCAACAAGCTG
59.97
TGGCTTGGACTTTCTCACCC
59.89
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTCCATCCTGTTGCCATGT
60.03
GGGGCAGGGACATGTACATT
59.74
172
PCR template sequence:
ATGTACCTCCATCCTGTTGCCATGTTTCGAGACCCCTTCCGCAAAGACCCCAACAAGCTGGTGCTATGTGAAGTTTTCAAGTATAACCGGAAACCTGCAGAACACCTTCCACCATGGCCACCTCAGCAAGTTCCCACTTGAACAAAGGCATCAAGCAAATGTACATGTCCCTGCCCCAGGGTGAGAAAGTCCAAGCCATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-431-5p(1), mmu-miR-494-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
CGGAAACCTGCAGAACACCTTCCACC
siRNA sets
AGGTGTTCTGCAGGTTTCC,AAGGTGTTCTGCAGGTTTC,TGGAAGGTGTTCTGCAGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCGLUL_341_ORF_1
0.8396
0.292614744
0.540281245
369
ORF sequence:
ATGGCCACCTCAGCAAGTTCCCACTTGAACAAAGGCATCAAGCAAATGTACATGTCCCTGCCCCAGGGTGAGAAAGTCCAAGCCATGTATATCTGGGTTGATGGTACCGGAGAAGGACTGCGCTGCAAGACCCGTACCCTGGACTGTGAGCCCAAGTGTGTGGAAGAGTTACCTGAGTGGAACTTTGATGGCTCTAGTACCTTTCAGTCTGAAGGCTCCAACAGCGACATGTACCTCCATCCTGTTGCCATGTTTCGAGACCCCTTCCGCAAAGACCCCAACAAGCTGGTGCTATGTGAAGTTTTCAAGTATAACCGGAAACCTGCAGAACACCTTCCACCATGGCCACCTCAGCAAGTTCCCACTTGA
Peptide sequence:
MATSASSHLNKGIKQMYMSLPQGEKVQAMYIWVDGTGEGLRCKTRTLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLHPVAMFRDPFRKDPNKLVLCEVFKYNRKPAEHLPPWPPQQVPT
IRESfinder result
IRESfinder score
0.377673739
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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