top of page
mmu_circFmnl2_479
Basic information of circular RNA
PanCircBase ID:
mmu_circFmnl2_479
mm39_Chr. Position ID:
chr2|52904814|52944618|+
Gene name:
Fmnl2
Transcript:
ENSMUST00000155586.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
2,3,4,5,6
Exon/intron offsets:
0,21701,27490,36397,39651
Genomic length:
39804
Exon/intron sizes in nt:
84,81,77,84,153
Flanking exon/introns:
chr2:52748271-52904814|chr2:52944618-52958513
Splice length:
479
Other database IDs:
circBase: mmu_circ_0010017, CIRCpedia: MMU_CIRCpedia_22495, circAtlas: mmu-Fmnl2_0014
circBase: hsa_circ_0117661, circAtlas: hsa-FMNL2_0014
Human Ortholog IDs:
Mature spliced Sequence:
AATGCTATGAACCTACCTCCTGACAAAGCCAGGCTACTGCGGCAGTATGACAATGAGAAAAAATGGGAGCTGATCTGTGATCAGGAGCGATTCCAGGTGAAGAACCCTCCCCATACGTACATACAGAAGCTCAAAGGCTACCTGGACCCAGCTGTAACCAGGAAGAAATTCAGAAGGCGTGTTCAAGAATCTACACAAGTGCTAAGAGAACTGGAAATTTCATTAAGAACCAACCACATTGGATGGGTCAGAGAGTTTTTGAATGAAGAAAACAAAGGCCTTGATGTTCTTGTGGAGTATTTGTCTTTTGCACAGTATGCAGTCACTTTTGACTTTGAAAGTGTGGAAAGTACCATGGAGAGTACGGTGGACAAATCAAAGCCCTGGAGCAGGTCCATCGAGGACCTGCACAGAGGGAGCAACCTGCCCTCACCTGTGGGCAACAGTGTGTCCCGCTCTGGAAGACATTCTGCACTTCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATCGAGGACCTGCACAGAG
60.18
CCTGATCACAGATCAGCTCCC
59.93
169
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CATCGAGGACCTGCACAGAG
60.18
TGGAATCGCTCCTGATCACAG
59.86
179
PCR template sequence:
AGCCCTGGAGCAGGTCCATCGAGGACCTGCACAGAGGGAGCAACCTGCCCTCACCTGTGGGCAACAGTGTGTCCCGCTCTGGAAGACATTCTGCACTTCGAATGCTATGAACCTACCTCCTGACAAAGCCAGGCTACTGCGGCAGTATGACAATGAGAAAAAATGGGAGCTGATCTGTGATCAGGAGCGATTCCAGGTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-miR-19b-3p(1), mmu-miR-19a-3p(1), mmu-let-7d-5p(1), mmu-miR-297a-5p(1), mmu-miR-98-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATTCTGCACTTCGAATGCTATGAACC
siRNA sets
GTTCATAGCATTCGAAGTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFMNL2_479_ORF_1
0.6797
0.194695221
0.569539284
483
ORF sequence:
ATGAACCTACCTCCTGACAAAGCCAGGCTACTGCGGCAGTATGACAATGAGAAAAAATGGGAGCTGATCTGTGATCAGGAGCGATTCCAGGTGAAGAACCCTCCCCATACGTACATACAGAAGCTCAAAGGCTACCTGGACCCAGCTGTAACCAGGAAGAAATTCAGAAGGCGTGTTCAAGAATCTACACAAGTGCTAAGAGAACTGGAAATTTCATTAAGAACCAACCACATTGGATGGGTCAGAGAGTTTTTGAATGAAGAAAACAAAGGCCTTGATGTTCTTGTGGAGTATTTGTCTTTTGCACAGTATGCAGTCACTTTTGACTTTGAAAGTGTGGAAAGTACCATGGAGAGTACGGTGGACAAATCAAAGCCCTGGAGCAGGTCCATCGAGGACCTGCACAGAGGGAGCAACCTGCCCTCACCTGTGGGCAACAGTGTGTCCCGCTCTGGAAGACATTCTGCACTTCGAATGCTATGA
Peptide sequence:
MNLPPDKARLLRQYDNEKKWELICDQERFQVKNPPHTYIQKLKGYLDPAVTRKKFRRRVQESTQVLRELEISLRTNHIGWVREFLNEENKGLDVLVEYLSFAQYAVTFDFESVESTMESTVDKSKPWSRSIEDLHRGSNLPSPVGNSVSRSGRHSALRML
IRESfinder result
IRESfinder score
0.547645951
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page