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mmu_circFbxo22_427
Basic information of circular RNA
PanCircBase ID:
mmu_circFbxo22_427
mm39_Chr. Position ID:
chr9|55122186|55128471|+
Gene name:
Fbxo22
Transcript:
ENSMUST00000034859.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,5,6
Exon/intron offsets:
0,3412,6119
Genomic length:
6285
Exon/intron sizes in nt:
9,61,65,166
Flanking exon/introns:
chr9:55121280-55122186|chr9:55128471-55130523
Splice length:
427
Other database IDs:
circAtlas: mmu-Fbxo22_0002
Human Ortholog IDs:
Mature spliced Sequence:
CGAGGAAGAGAACTACTATGGAGACAGCATGTGCCCTGGAGAAGCTTTTCCCCAAGCAGTGCCAAGTCCTTGGGATTGTGACCCCGGGAATTGTGGTGACTCCAATGGGATCAGGTAGCAATCGACCTCAGGAAATAGAAATTGGAGAATCTGGTTTTGCTTTATTATTCCCTCAAATTGAAGGAATAAAAATTCAACCCTTTCATTTTATTAAGGACTCCAAGAATTTAACACTCGAAAGACACCAGCTTACTGAAGTAGGTCTTCTGGACAACCCTGAACTTCGTGTGGTCCTTGTCTTTGGCTATAACTGCTGTAAGGTGGGAGCCAGTAATTACCTGCATCGGGTAGTCAGCACTTTCAGCGATATGAATATCATCTTGGCTGGAGGCCAGGTGGACAATTTGTCTTCACTTACTTGTGAGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCATCGGGTAGTCAGCACTT
60.11
AATTCCCGGGGTCACAATCC
60.03
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCATCGGGTAGTCAGCACTT
60.11
CAAGGACTTGGCACTGCTTG
59.69
159
PCR template sequence:
CCAGTAATTACCTGCATCGGGTAGTCAGCACTTTCAGCGATATGAATATCATCTTGGCTGGAGGCCAGGTGGACAATTTGTCTTCACTTACTTGTGAGAACGAGGAAGAGAACTACTATGGAGACAGCATGTGCCCTGGAGAAGCTTTTCCCCAAGCAGTGCCAAGTCCTTGGGATTGTGACCCCGGGAATTGTGGTGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-106b-5p(1), mmu-miR-149-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTACTTGTGAGAACGAGGAAGAGAAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFBXO22_427_ORF_1
0.8579
0.116315217
0.649782564
528
ORF sequence:
ATGGAGACAGCATGTGCCCTGGAGAAGCTTTTCCCCAAGCAGTGCCAAGTCCTTGGGATTGTGACCCCGGGAATTGTGGTGACTCCAATGGGATCAGGTAGCAATCGACCTCAGGAAATAGAAATTGGAGAATCTGGTTTTGCTTTATTATTCCCTCAAATTGAAGGAATAAAAATTCAACCCTTTCATTTTATTAAGGACTCCAAGAATTTAACACTCGAAAGACACCAGCTTACTGAAGTAGGTCTTCTGGACAACCCTGAACTTCGTGTGGTCCTTGTCTTTGGCTATAACTGCTGTAAGGTGGGAGCCAGTAATTACCTGCATCGGGTAGTCAGCACTTTCAGCGATATGAATATCATCTTGGCTGGAGGCCAGGTGGACAATTTGTCTTCACTTACTTGTGAGAACGAGGAAGAGAACTACTATGGAGACAGCATGTGCCCTGGAGAAGCTTTTCCCCAAGCAGTGCCAAGTCCTTGGGATTGTGACCCCGGGAATTGTGGTGACTCCAATGGGATCAGGTAG
Peptide sequence:
METACALEKLFPKQCQVLGIVTPGIVVTPMGSGSNRPQEIEIGESGFALLFPQIEGIKIQPFHFIKDSKNLTLERHQLTEVGLLDNPELRVVLVFGYNCCKVGASNYLHRVVSTFSDMNIILAGGQVDNLSSLTCENEEENYYGDSMCPGEAFPQAVPSPWDCDPGNCGDSNGIR
IRESfinder result
IRESfinder score
0.824456303
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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