top of page
mmu_circFars2_292
Basic information of circular RNA
PanCircBase ID:
mmu_circFars2_292
mm39_Chr. Position ID:
chr13|36416104|36430477|+
Gene name:
Fars2
Transcript:
ENSMUST00000021857.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,14241
Genomic length:
14373
Exon/intron sizes in nt:
160,132
Flanking exon/introns:
chr13:36389124-36416104|chr13:36430477-36525919
Splice length:
292
Other database IDs:
circBase: mmu_circ_0000453, CIRCpedia: MMU_CIRCpedia_18072, circAtlas: mmu-Fars2_0001
circBase: hsa_circ_0075533, circAtlas: hsa-FARS2_0001, CIRCpedia: HSA_CIRCpedia_55298
Human Ortholog IDs:
Mature spliced Sequence:
TTATTTGCTGGCGTAAAGGATGGAGAAAGCTTGCAGCTCTTTGAAGAAGGTTCTCGCTCTGCTCATAAGCAGGAGACACACACCATGGAGGCTGTGAAGCTTGTGGAGTTCGACCTTAAACAAGTACTGACTAGGCTTGTGACACATCTTTTTGGAGATGGGCTGGAGGTTCGGTGGGTGGATTGCTACTTCCCCTTCACCCACCCTTCCTTCGAGATGGAGATCAACTTCCGTGGAGAATGGCTTGAAGTCCTTGGCTGCGGTGTGATGGAGCAGCAGCTTGTCAATTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCACCCTTCCTTCGAGATGG
59.82
CATGGTGTGTGTCTCCTGCT
59.96
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCACCCTTCCTTCGAGATG
59.82
CATGGTGTGTGTCTCCTGCT
59.96
180
PCR template sequence:
CCCTTCACCCACCCTTCCTTCGAGATGGAGATCAACTTCCGTGGAGAATGGCTTGAAGTCCTTGGCTGCGGTGTGATGGAGCAGCAGCTTGTCAATTCAGTTATTTGCTGGCGTAAAGGATGGAGAAAGCTTGCAGCTCTTTGAAGAAGGTTCTCGCTCTGCTCATAAGCAGGAGACACACACCATGGAGGCTGTGAAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTGTCAATTCAGTTATTTGCTGGCG
siRNA sets
AAATAACTGAATTGACAAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFARS2_292_ORF_1
1.0124
0.125067038
0.260339543
252
ORF sequence:
ATGGAGGCTGTGAAGCTTGTGGAGTTCGACCTTAAACAAGTACTGACTAGGCTTGTGACACATCTTTTTGGAGATGGGCTGGAGGTTCGGTGGGTGGATTGCTACTTCCCCTTCACCCACCCTTCCTTCGAGATGGAGATCAACTTCCGTGGAGAATGGCTTGAAGTCCTTGGCTGCGGTGTGATGGAGCAGCAGCTTGTCAATTCAGTTATTTGCTGGCGTAAAGGATGGAGAAAGCTTGCAGCTCTTTGA
Peptide sequence:
MEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEINFRGEWLEVLGCGVMEQQLVNSVICWRKGWRKLAAL
IRESfinder result
IRESfinder score
0.578164563
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page