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mmu_circFam184a_1883
Basic information of circular RNA
PanCircBase ID:
mmu_circFam184a_1883
mm39_Chr. Position ID:
chr10|53517040|53570972|-
Gene name:
Fam184a
Transcript:
ENSMUST00000020003.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
15,14,13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,6038,6627,8157,11355,14022,33621,34191,36786,43291,49006,53750
Genomic length:
53932
Exon/intron sizes in nt:
1,18,14,71,85,12,81,54,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr10:53514377-53517040|chr10:53570972-53573219
Splice length:
1883
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCATATTGGAATGCTTCAGGCAACTCAGATGACCCAAGAAGTTACCATTAAAGATTTAGAATCAGAAAAATCCAGAGCCAACGAGCGATTGTGTCAGCTGGAAGAGGAAAGAGCTTTTTTGCAGAGCAGAACGCAGAGTCTGGATGAAGAACAGAAGCAGCAGGTGCTGGAGCTGGAGAAGAAAGTAAATGAAGCAAAGAGAACTCAGCAAGAATATTACGAAATGGAGCTTAAGAACCTGCAAAATCGATTGGAAGGAGAGGTGGCTCAGTTAAACGAGGCCCATGGGAAGACTTTGGAAGAATTAGCTCGGAAGCACCACATGGCGATTGAAGCTGTCCATAGCAATGCAAGCAGAGATAAGATAAAGCTACAGACGGAATTGGAGGAACAATATAAAAAGGAGAAACTGAGTCTGGAAGAGGATAAAAATCAGCTCCAACTAGAGCTGGAAAGTCTAAAGCAAGCTCTGGGAGACAAGCTGACTTCAGCCAATCAGGAGATTGGCCGCCTCCAGGATCTCGTAAGGAAGAGTGAACAAGGTCTCGGCTCTGCCGAAGGACTCATTTCTAGTCTTCAGGATTCCCAGGAAAGGCTTCAGAGTGAGCTGGACCTGACAAAAGGCAGGCTGAAGGAGACCAAGGATGCCCTATTAAATGTTGAGGCTGAGCTACAACAAGAACGTCATGAGCATGAGCAGACGCTGGCCACCATGAAGGAAGAAGAGAAGCTGAGGGTGGACAGAATGGCCCATGACCTGGAGATCAAGTGGACTGAAAATCTTAGACAAGAGTGCTCCAAGCTTCGCCAGGAGTTGAGGCTTCAACACGAAGAAGATAAAAAGTCTGCCATGTCTCAACTGTTGCAGCTGAAAGAGAGAGAGAAAAATGCCGCCAGGGACTCATGGCAGAAGAAGGTGGAAGATCTCTTAAACCAGATCTCCCTGCTGAAGCAGAACCTGGAGCTGCAGCTTTGCCAGTCCCAGACGTCTCTGCAGCAGCTGCAAGCGCAGTTCACGCAAGAGCGCCAGCGACTCACGCAGGAGCTGGAGGAGCTGGAGGAGCAGCATCAGCAGAGGCACAAGTCCCTCAAAGAAGCACACGTCCTCGCGTTCCAGACCATGGAAGAAGAGAAGGAAAAGGAGCAAAGGGCTCTGGAGACTCACTTACAGCAAAAGCATTCTGCAGAGCTGCAGTCACTGAAAGATGCGCACCGAGAGTCGATGGAGGGCTTCCGCGTGGAGATGGAGCAGGAGCTTCAGACACTCCGGTTTGAGTTAGAAGACGAGGGCAAGGCTATGCTTGCTTCCTTGCGTTCTGAACTAAACCATCAACATGCGGCTTCCATTGATTTATTACGGCATAGTCACCATCAAGAACTGGCAGCAGCTAAGATGGAATTAGAGAGAAGCATAGACATCAGCCGCAGGCAGAGTAAGGAGCACATGTGTAGGATAAGCGATCTGCAGGAGGAGCTGAGGCACCGGGAGCACCACATCACAGACTTGGATAAGGAGGTCCAGCACCTCCATGAAAACATAAATACCCTAACGAAAGAACTGGAACTCAAGGGGAAAGAGATTCTCAGAGTACGGAGTGAATCCAACCAGCAAATGAGGTTGCATGAACAAGATTTAAACAAGAGACTTGAGAAAGAGCTGGATGTCATGACAGCAGACCACCTCAGAGAGAAAAATATCATGCGGGCAGATTTTAATAAGACTAATGAGCTACTCAAGGAAATAAATGCAGCTTTACAAGTGTCACTAGAAGATATGGAAGAGAAGTATCTAATGAGAGAATCAAGGCCAGAAGATATACAGATGATTGCAGAATTAAAATCCCTGATAACAGAACGAGACCAGGTCATAAAGAAACTCATT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTGATAACAGAACGAGACCA
59.5
GCTGACACAATCGCTCGTTG
60.18
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTGATAACAGAACGAGACCA
59.5
AGCTGACACAATCGCTCGTT
60.32
141
PCR template sequence:
GAAGTATCTAATGAGAGAATCAAGGCCAGAAGATATACAGATGATTGCAGAATTAAAATCCCTGATAACAGAACGAGACCAGGTCATAAAGAAACTCATTGTCATATTGGAATGCTTCAGGCAACTCAGATGACCCAAGAAGTTACCATTAAAGATTTAGAATCAGAAAAATCCAGAGCCAACGAGCGATTGTGTCAGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-1904(2), mmu-miR-134-5p(1), mmu-miR-320-3p(1), mmu-miR-127-5p(1), mmu-miR-758-3p(1), mmu-miR-541-5p(1), mmu-miR-425-5p(1), mmu-miR-1198-5p(1), mmu-miR-207(1), mmu-miR-5101(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAGAAACTCATTGTCATATTGGAAT
siRNA sets
ATTCCAATATGACAATGAG, TTCCAATATGACAATGAGT, AATATGACAATGAGTTTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFAM184A_1883_ORF_1
1.2377
0.333495468
0.999994335
1905
ORF sequence:
ATGCTTCAGGCAACTCAGATGACCCAAGAAGTTACCATTAAAGATTTAGAATCAGAAAAATCCAGAGCCAACGAGCGATTGTGTCAGCTGGAAGAGGAAAGAGCTTTTTTGCAGAGCAGAACGCAGAGTCTGGATGAAGAACAGAAGCAGCAGGTGCTGGAGCTGGAGAAGAAAGTAAATGAAGCAAAGAGAACTCAGCAAGAATATTACGAAATGGAGCTTAAGAACCTGCAAAATCGATTGGAAGGAGAGGTGGCTCAGTTAAACGAGGCCCATGGGAAGACTTTGGAAGAATTAGCTCGGAAGCACCACATGGCGATTGAAGCTGTCCATAGCAATGCAAGCAGAGATAAGATAAAGCTACAGACGGAATTGGAGGAACAATATAAAAAGGAGAAACTGAGTCTGGAAGAGGATAAAAATCAGCTCCAACTAGAGCTGGAAAGTCTAAAGCAAGCTCTGGGAGACAAGCTGACTTCAGCCAATCAGGAGATTGGCCGCCTCCAGGATCTCGTAAGGAAGAGTGAACAAGGTCTCGGCTCTGCCGAAGGACTCATTTCTAGTCTTCAGGATTCCCAGGAAAGGCTTCAGAGTGAGCTGGACCTGACAAAAGGCAGGCTGAAGGAGACCAAGGATGCCCTATTAAATGTTGAGGCTGAGCTACAACAAGAACGTCATGAGCATGAGCAGACGCTGGCCACCATGAAGGAAGAAGAGAAGCTGAGGGTGGACAGAATGGCCCATGACCTGGAGATCAAGTGGACTGAAAATCTTAGACAAGAGTGCTCCAAGCTTCGCCAGGAGTTGAGGCTTCAACACGAAGAAGATAAAAAGTCTGCCATGTCTCAACTGTTGCAGCTGAAAGAGAGAGAGAAAAATGCCGCCAGGGACTCATGGCAGAAGAAGGTGGAAGATCTCTTAAACCAGATCTCCCTGCTGAAGCAGAACCTGGAGCTGCAGCTTTGCCAGTCCCAGACGTCTCTGCAGCAGCTGCAAGCGCAGTTCACGCAAGAGCGCCAGCGACTCACGCAGGAGCTGGAGGAGCTGGAGGAGCAGCATCAGCAGAGGCACAAGTCCCTCAAAGAAGCACACGTCCTCGCGTTCCAGACCATGGAAGAAGAGAAGGAAAAGGAGCAAAGGGCTCTGGAGACTCACTTACAGCAAAAGCATTCTGCAGAGCTGCAGTCACTGAAAGATGCGCACCGAGAGTCGATGGAGGGCTTCCGCGTGGAGATGGAGCAGGAGCTTCAGACACTCCGGTTTGAGTTAGAAGACGAGGGCAAGGCTATGCTTGCTTCCTTGCGTTCTGAACTAAACCATCAACATGCGGCTTCCATTGATTTATTACGGCATAGTCACCATCAAGAACTGGCAGCAGCTAAGATGGAATTAGAGAGAAGCATAGACATCAGCCGCAGGCAGAGTAAGGAGCACATGTGTAGGATAAGCGATCTGCAGGAGGAGCTGAGGCACCGGGAGCACCACATCACAGACTTGGATAAGGAGGTCCAGCACCTCCATGAAAACATAAATACCCTAACGAAAGAACTGGAACTCAAGGGGAAAGAGATTCTCAGAGTACGGAGTGAATCCAACCAGCAAATGAGGTTGCATGAACAAGATTTAAACAAGAGACTTGAGAAAGAGCTGGATGTCATGACAGCAGACCACCTCAGAGAGAAAAATATCATGCGGGCAGATTTTAATAAGACTAATGAGCTACTCAAGGAAATAAATGCAGCTTTACAAGTGTCACTAGAAGATATGGAAGAGAAGTATCTAATGAGAGAATCAAGGCCAGAAGATATACAGATGATTGCAGAATTAAAATCCCTGATAACAGAACGAGACCAGGTCATAAAGAAACTCATTGTCATATTGGAATGCTTCAGGCAACTCAGATGA
Peptide sequence:
MLQATQMTQEVTIKDLESEKSRANERLCQLEEERAFLQSRTQSLDEEQKQQVLELEKKVNEAKRTQQEYYEMELKNLQNRLEGEVAQLNEAHGKTLEELARKHHMAIEAVHSNASRDKIKLQTELEEQYKKEKLSLEEDKNQLQLELESLKQALGDKLTSANQEIGRLQDLVRKSEQGLGSAEGLISSLQDSQERLQSELDLTKGRLKETKDALLNVEAELQQERHEHEQTLATMKEEEKLRVDRMAHDLEIKWTENLRQECSKLRQELRLQHEEDKKSAMSQLLQLKEREKNAARDSWQKKVEDLLNQISLLKQNLELQLCQSQTSLQQLQAQFTQERQRLTQELEELEEQHQQRHKSLKEAHVLAFQTMEEEKEKEQRALETHLQQKHSAELQSLKDAHRESMEGFRVEMEQELQTLRFELEDEGKAMLASLRSELNHQHAASIDLLRHSHHQELAAAKMELERSIDISRRQSKEHMCRISDLQEELRHREHHITDLDKEVQHLHENINTLTKELELKGKEILRVRSESNQQMRLHEQDLNKRLEKELDVMTADHLREKNIMRADFNKTNELLKEINAALQVSLEDMEEKYLMRESRPEDIQMIAELKSLITERDQVIKKLIVILECFRQLR
IRESfinder result
IRESfinder score
0.329900466
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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