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mmu_circFam114a2_1364
Basic information of circular RNA
PanCircBase ID:
mmu_circFam114a2_1364
mm39_Chr. Position ID:
chr11|57378392|57405114|-
Gene name:
Fam114a2
Transcript:
ENSMUST00000020831.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,2543,4353,5437,12159,17667,19270,19926,21811,25675,26069,26528
Genomic length:
26722
Exon/intron sizes in nt:
54,73,140,123,80,124,159,135,89,93,100,194
Flanking exon/introns:
chr11:57374966-57378392|chr11:57405114-57409398
Splice length:
1364
Other database IDs:
circAtlas: mmu-Fam114a2_0028
circAtlas: hsa-FAM114A2_0016, CIRCpedia: HSA_CIRCpedia_102145
Human Ortholog IDs:
Mature spliced Sequence:
GCGATGTTGTAGTCATGTCAGATAAGGATCCTCCAGAGTCTCCAGTGGTAACCGGAGTAGCTTCGACCCTTAAAGATGAGAACTGTGAGCCAGTGGAGAAGCCAGAGGATAAATCTCAGCCTGTAGTGTCCACCCGGAAAAGACCAGAGACCAAACCTTCCAGTGACCTTGAGGCTTCTGCTCTTCCTGCTCAGGAGACTGCTTCTAAAGATGTACCTCAGACTGGGTGGGGTTATTGGGGCAGTTGGGGCAAGTCTCTGCTCTCCTCAGCCTCAGCTACGGTAGCTACAGTAGGACAAGGTATTTCAAATGTCATTGAGAAGGCAGAGACTTCTCTTGGGATTCCTAGCCCCACTGAAATTTCAGCTGAAGTCAAGCAAGCAGCAGGAGAAAAAAATGCTGGTGAGAATGGAAGCTTACTAGTGGCTGCACCATTCGGGATGCTCTCGACCATCTCTACAGCTGTGCAGAGCACGGGCAAGAGCGTTATCAGTGGGGGCTTGGATGCCTTGGAATTCATTGGGAAGAAGACAATGGATGTCATAGCAGAAGGGGACCCTGGATTTAAAAGAACCAAGGGCCTGATGAATCGGACTTCTACGCTGTCTCAGGTTTTACGAGAGGCAAAGGATAAAGAAGAGCAGCGGCCTTCCAATGAAGTAACCATGGAAACAGACAAGAAAACTCATTATGGGCTACTCTTTGATGAGTTTCAAGGCCTTTCACATCTAGAAGCTCTGGAGATGCTTTCCCGAGAAAGTGAGATAAAGGTGAAATCTATTCTTAGTTCTCTGAGTGGAGAAGAACTACAGACAACTAGACTTGAGCTAGAGCAGCTCAAAGAAGTGTTTTCCCTAGCAGAGTTCTGTGAGGAAGAGGAGGAGGAGAGACAAGGGGATGACAATTTTACCAAGGAGATAACAGATCTGTTTGCTCAGCTTCACGTCTCCTCCCGACCAGAGAAGCTTGCCAGGGCAAGAAATACAGCCTACAAATGGATCAGAACATCTCTGGCTAGGCCAGTAGCAGAGAAAGAAGAAGGAGAAAAAGAGTCGGAGGCAGGAAATACCGAGGAAGCCCAGAAACATTCAATAGAGGATATCCATGCATTTGCAATCCGGAGCTTAGCAGAGTTGACTGCCTGCTCAATAGAATTGTTCCACAAAACAGCAGCATTGGTTTTGCATGGCCAGAAGCAGGAGGTGACAGCCTTAGAGAGGAGCCAGACTCTCTCCCAGATGACAGTCATGCTGTGTAAAGACCTGGCCTCGCTGTCTAAAGAGTTCACCACCTGCCTGACCACAGCTGGGGTCAGAGAGAAGGCAGATGTTCTTAATCCAGTCATCACTGCAGTGTTTCTAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCTCGCTGTCTAAAGAGT
59.47
CCACTGGAGACTCTGGAGGA
59.96
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCTCGCTGTCTAAAGAGT
59.47
ACCACTGGAGACTCTGGAGG
60.25
149
PCR template sequence:
GGCCTCGCTGTCTAAAGAGTTCACCACCTGCCTGACCACAGCTGGGGTCAGAGAGAAGGCAGATGTTCTTAATCCAGTCATCACTGCAGTGTTTCTAGAGGCGATGTTGTAGTCATGTCAGATAAGGATCCTCCAGAGTCTCCAGTGGTAACCGGAGTAGCTTCGACCCTTAAAGATGAGAACTGTGAGCCAGTGGAGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(2), mmu-miR-207(2), mmu-miR-200a-3p(1), mmu-miR-211-5p(1), mmu-miR-127-5p(1), mmu-miR-452-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTGTTTCTAGAGGCGATGTTGTAGT
siRNA sets
ACATCGCCTCTAGAAACAC, CAACATCGCCTCTAGAAAC, TACAACATCGCCTCTAGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFAM114A2_1364_ORF_1
0.9756
0.206773973
0.999240014
1362
ORF sequence:
ATGTCAGATAAGGATCCTCCAGAGTCTCCAGTGGTAACCGGAGTAGCTTCGACCCTTAAAGATGAGAACTGTGAGCCAGTGGAGAAGCCAGAGGATAAATCTCAGCCTGTAGTGTCCACCCGGAAAAGACCAGAGACCAAACCTTCCAGTGACCTTGAGGCTTCTGCTCTTCCTGCTCAGGAGACTGCTTCTAAAGATGTACCTCAGACTGGGTGGGGTTATTGGGGCAGTTGGGGCAAGTCTCTGCTCTCCTCAGCCTCAGCTACGGTAGCTACAGTAGGACAAGGTATTTCAAATGTCATTGAGAAGGCAGAGACTTCTCTTGGGATTCCTAGCCCCACTGAAATTTCAGCTGAAGTCAAGCAAGCAGCAGGAGAAAAAAATGCTGGTGAGAATGGAAGCTTACTAGTGGCTGCACCATTCGGGATGCTCTCGACCATCTCTACAGCTGTGCAGAGCACGGGCAAGAGCGTTATCAGTGGGGGCTTGGATGCCTTGGAATTCATTGGGAAGAAGACAATGGATGTCATAGCAGAAGGGGACCCTGGATTTAAAAGAACCAAGGGCCTGATGAATCGGACTTCTACGCTGTCTCAGGTTTTACGAGAGGCAAAGGATAAAGAAGAGCAGCGGCCTTCCAATGAAGTAACCATGGAAACAGACAAGAAAACTCATTATGGGCTACTCTTTGATGAGTTTCAAGGCCTTTCACATCTAGAAGCTCTGGAGATGCTTTCCCGAGAAAGTGAGATAAAGGTGAAATCTATTCTTAGTTCTCTGAGTGGAGAAGAACTACAGACAACTAGACTTGAGCTAGAGCAGCTCAAAGAAGTGTTTTCCCTAGCAGAGTTCTGTGAGGAAGAGGAGGAGGAGAGACAAGGGGATGACAATTTTACCAAGGAGATAACAGATCTGTTTGCTCAGCTTCACGTCTCCTCCCGACCAGAGAAGCTTGCCAGGGCAAGAAATACAGCCTACAAATGGATCAGAACATCTCTGGCTAGGCCAGTAGCAGAGAAAGAAGAAGGAGAAAAAGAGTCGGAGGCAGGAAATACCGAGGAAGCCCAGAAACATTCAATAGAGGATATCCATGCATTTGCAATCCGGAGCTTAGCAGAGTTGACTGCCTGCTCAATAGAATTGTTCCACAAAACAGCAGCATTGGTTTTGCATGGCCAGAAGCAGGAGGTGACAGCCTTAGAGAGGAGCCAGACTCTCTCCCAGATGACAGTCATGCTGTGTAAAGACCTGGCCTCGCTGTCTAAAGAGTTCACCACCTGCCTGACCACAGCTGGGGTCAGAGAGAAGGCAGATGTTCTTAATCCAGTCATCACTGCAGTGTTTCTAGAGGCGATGTTGTAG
Peptide sequence:
MSDKDPPESPVVTGVASTLKDENCEPVEKPEDKSQPVVSTRKRPETKPSSDLEASALPAQETASKDVPQTGWGYWGSWGKSLLSSASATVATVGQGISNVIEKAETSLGIPSPTEISAEVKQAAGEKNAGENGSLLVAAPFGMLSTISTAVQSTGKSVISGGLDALEFIGKKTMDVIAEGDPGFKRTKGLMNRTSTLSQVLREAKDKEEQRPSNEVTMETDKKTHYGLLFDEFQGLSHLEALEMLSRESEIKVKSILSSLSGEELQTTRLELEQLKEVFSLAEFCEEEEEERQGDDNFTKEITDLFAQLHVSSRPEKLARARNTAYKWIRTSLARPVAEKEEGEKESEAGNTEEAQKHSIEDIHAFAIRSLAELTACSIELFHKTAALVLHGQKQEVTALERSQTLSQMTVMLCKDLASLSKEFTTCLTTAGVREKADVLNPVITAVFLEAML
IRESfinder result
IRESfinder score
0.556993512
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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