top of page
mmu_circEya1_351
Basic information of circular RNA
PanCircBase ID:
mmu_circEya1_351
mm39_Chr. Position ID:
chr1|14340928|14344827|-
Gene name:
Eya1
Transcript:
ENSMUST00000027066.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,6,5
Exon/intron offsets:
0,3360,3829
Genomic length:
3899
Exon/intron sizes in nt:
138,143,70
Flanking exon/introns:
chr1:14323326-14340928|chr1:14344827-14353344
Splice length:
351
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_141557
Human Ortholog IDs:
Mature spliced Sequence:
CTCTCGGAAGCAGCAGCTTTAGTCCAAGACCAGCTCACCCGTTCTCTCCACCACAGATTTATCCTTCCAAATCATACCCACATATTCTCCCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGGAATGCAACAAGCCACAGCCTACGCCACGTACCCACAGCCTGGACAGCCCTATGGAATTTCCTCCTATGGTGCATTGTGGGCAGGCATCAAGACGGAAAGTGGATTGTCACAGTCTCAGTCACCTGGACAGACGGGATTTCTTAGCTATGGCACAAGCTTTGGTACCCCTCAACCTGGACAGGCACCGTACAGCTACCAGATGCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACCTGGACAGACGGGATTT
59.68
ATCTGTGGTGGAGAGAACGG
59.1
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACCTGGACAGACGGGATTT
59.68
GTGGTGGAGAGAACGGGTG
60
141
PCR template sequence:
TCACAGTCTCAGTCACCTGGACAGACGGGATTTCTTAGCTATGGCACAAGCTTTGGTACCCCTCAACCTGGACAGGCACCGTACAGCTACCAGATGCAAGCTCTCGGAAGCAGCAGCTTTAGTCCAAGACCAGCTCACCCGTTCTCTCCACCACAGATTTATCCTTCCAAATCATACCCACATATTCTCCCTACCCCTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-320-3p(1), mmu-miR-185-5p(1), mmu-miR-501-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TACCAGATGCAAGCTCTCGGAAGCAG
siRNA sets
TTCCGAGAGCTTGCATCTG, CTTCCGAGAGCTTGCATCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEYA1_351_ORF_1
0.7362
0.124996992
0.707946149
591
ORF sequence:
ATGGCTGCATATGGGCAAACACAGTTTACCACAGGAATGCAACAAGCCACAGCCTACGCCACGTACCCACAGCCTGGACAGCCCTATGGAATTTCCTCCTATGGTGCATTGTGGGCAGGCATCAAGACGGAAAGTGGATTGTCACAGTCTCAGTCACCTGGACAGACGGGATTTCTTAGCTATGGCACAAGCTTTGGTACCCCTCAACCTGGACAGGCACCGTACAGCTACCAGATGCAAGCTCTCGGAAGCAGCAGCTTTAGTCCAAGACCAGCTCACCCGTTCTCTCCACCACAGATTTATCCTTCCAAATCATACCCACATATTCTCCCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGGAATGCAACAAGCCACAGCCTACGCCACGTACCCACAGCCTGGACAGCCCTATGGAATTTCCTCCTATGGTGCATTGTGGGCAGGCATCAAGACGGAAAGTGGATTGTCACAGTCTCAGTCACCTGGACAGACGGGATTTCTTAGCTATGGCACAAGCTTTGGTACCCCTCAACCTGGACAGGCACCGTACAGCTACCAGATGCAA
Peptide sequence:
MAAYGQTQFTTGMQQATAYATYPQPGQPYGISSYGALWAGIKTESGLSQSQSPGQTGFLSYGTSFGTPQPGQAPYSYQMQALGSSSFSPRPAHPFSPPQIYPSKSYPHILPTPSSQTMAAYGQTQFTTGMQQATAYATYPQPGQPYGISSYGALWAGIKTESGLSQSQSPGQTGFLSYGTSFGTPQPGQAPYSYQMQ
IRESfinder result
IRESfinder score
0.574295999
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page