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mmu_circEvi5_889
Basic information of circular RNA
PanCircBase ID:
mmu_circEvi5_889
mm39_Chr. Position ID:
chr5|107912541|107961472|-
Gene name:
Evi5
Transcript:
ENSMUST00000112642.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
17,16,15,14,13,12,11,10
Exon/intron offsets:
0,23523,25686,31023,33137,34450,47682,48870
Genomic length:
48931
Exon/intron sizes in nt:
96,96,147,159,141,135,54,61
Flanking exon/introns:
chr5:107896323-107912541|chr5:107961472-107963476
Splice length:
889
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
ACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAACATAAATGCAGTTCCACCTACAACGAAGATTTCGTGCTGCAGCTGGAGAAGGAATTGGTTCAAGCCAGACTGAGTGAAGCCGAGTCTCAGTGTGCACTGAAGGAGATGCAGGATAAAGTGTTGGATATAGAGAAGAAGAACAACTCATTTCCTGATGAGAATAATATTGCAAGGCTTCAGGAGGAACTCATCGCTGTGAAGCTGAGGGAAGCCGAAGCCATTATGGGTTTGAAAGAACTTCGACAGCAAGTCAGAACCTTAGAAGAGCACTGGCAGCGTCACTTAGCTCGAACTTCTGGAAGATGGAAAGACCCACCCAAGAAAAATGCTGTGAATGAGTTACAGGATGAGCTGATGAGCATTCGCCTTAGAGAGGCGGAAACACAGGCAGAAATAAGAGAGATGAAGCAAAGGATGATGGAAATGGAGACACAGAACCAGATCAATAGTAACCAACTTCGAAGAGCGGAGCAAGAGGTGAACAGTCTGCAGGAGAAGGTGTGCTCGCTGTCTGTGAAGAACAAGGGCTTGCTTGCCCAGTTAAGTGAGGCAAAGCGCAGACAGGCAGAGATTGAATGCAAGAACAAGGAGGAAGTCATGGCTGTGCGGCTCCGGGAAGCTGATAGCATCGCGGCCGTGGCTGAGCTGCAGCAGCACATTGCTGAGCTTGAAATCCAGAAAGAAGAGGGCAAGCTCCAAGGACAGCTTAACAGGTCTGACTCTAACCAGTACATTAGGGAACTGAAGGACCAGATAGCAGAGCTGACTCATGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAAGAGGGCAAGCTCCAAG
59.67
AAAGCCTATTTTCTGTGCGT
55.95
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGAGGGCAAGCTCCAAGG
60.68
AAAGCCTATTTTCTGTGCGT
55.95
179
PCR template sequence:
CCAGAAAGAAGAGGGCAAGCTCCAAGGACAGCTTAACAGGTCTGACTCTAACCAGTACATTAGGGAACTGAAGGACCAGATAGCAGAGCTGACTCATGAGACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-27a-3p(1), mmu-miR-135a-5p(1), mmu-miR-211-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-207(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTGACTCATGAGACTTGAAAAAGAA
siRNA sets
TTTTCAAGTCTCATGAGTC, TTTCAAGTCTCATGAGTCA, TTCAAGTCTCATGAGTCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEVI5_889_ORF_1
1.2359
0.343847378
0.992392003
894
ORF sequence:
ATGAGACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAACATAAATGCAGTTCCACCTACAACGAAGATTTCGTGCTGCAGCTGGAGAAGGAATTGGTTCAAGCCAGACTGAGTGAAGCCGAGTCTCAGTGTGCACTGAAGGAGATGCAGGATAAAGTGTTGGATATAGAGAAGAAGAACAACTCATTTCCTGATGAGAATAATATTGCAAGGCTTCAGGAGGAACTCATCGCTGTGAAGCTGAGGGAAGCCGAAGCCATTATGGGTTTGAAAGAACTTCGACAGCAAGTCAGAACCTTAGAAGAGCACTGGCAGCGTCACTTAGCTCGAACTTCTGGAAGATGGAAAGACCCACCCAAGAAAAATGCTGTGAATGAGTTACAGGATGAGCTGATGAGCATTCGCCTTAGAGAGGCGGAAACACAGGCAGAAATAAGAGAGATGAAGCAAAGGATGATGGAAATGGAGACACAGAACCAGATCAATAGTAACCAACTTCGAAGAGCGGAGCAAGAGGTGAACAGTCTGCAGGAGAAGGTGTGCTCGCTGTCTGTGAAGAACAAGGGCTTGCTTGCCCAGTTAAGTGAGGCAAAGCGCAGACAGGCAGAGATTGAATGCAAGAACAAGGAGGAAGTCATGGCTGTGCGGCTCCGGGAAGCTGATAGCATCGCGGCCGTGGCTGAGCTGCAGCAGCACATTGCTGAGCTTGAAATCCAGAAAGAAGAGGGCAAGCTCCAAGGACAGCTTAACAGGTCTGACTCTAACCAGTACATTAGGGAACTGAAGGACCAGATAGCAGAGCTGACTCATGAG
Peptide sequence:
MRLEKEYTTIKTKEMEEQGEIKRLRTENRLLKQRIETLEKHKCSSTYNEDFVLQLEKELVQARLSEAESQCALKEMQDKVLDIEKKNNSFPDENNIARLQEELIAVKLREAEAIMGLKELRQQVRTLEEHWQRHLARTSGRWKDPPKKNAVNELQDELMSIRLREAETQAEIREMKQRMMEMETQNQINSNQLRRAEQEVNSLQEKVCSLSVKNKGLLAQLSEAKRRQAEIECKNKEEVMAVRLREADSIAAVAELQQHIAELEIQKEEGKLQGQLNRSDSNQYIRELKDQIAELTHE
IRESfinder result
IRESfinder score
0.326665767
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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