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mmu_circEvi5_306
Basic information of circular RNA
PanCircBase ID:
mmu_circEvi5_306
mm39_Chr. Position ID:
chr5|107938227|107943723|-
Gene name:
Evi5
Transcript:
ENSMUST00000112642.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
15,14
Exon/intron offsets:
0,5337
Genomic length:
5496
Exon/intron sizes in nt:
1,47,159
Flanking exon/introns:
chr5:107936160-107938227|chr5:107943723-107945678
Splice length:
306
Other database IDs:
circAtlas: mmu-Evi5_0051
Human Ortholog IDs:
Mature spliced Sequence:
CGTCACTTAGCTCGAACTTCTGGAAGATGGAAAGACCCACCCAAGAAAAATGCTGTGAATGAGTTACAGGATGAGCTGATGAGCATTCGCCTTAGAGAGGCGGAAACACAGGCAGAAATAAGAGAGATGAAGCAAAGGATGATGGAAATGGAGACACAGAACCAGATCAATAGTAACCAACTTCGAAGAGCGGAGCAAGAGGTGAACAGTCTGCAGGAGAAGGTGTGCTCGCTGTCTGTGAAGAACAAGGGCTTGCTTGCCCAGTTAAGTGAGGCAAAGCGCAGACAGGCAGAGATTGAATGCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TAAGTGAGGCAAAGCGCAGA
59.97
CCTCTCTAAGGCGAATGCTCA
59.59
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTTGCTTGCCCAGTTAAG
59.76
CCTCTCTAAGGCGAATGCTCA
59.59
157
PCR template sequence:
CAGTCTGCAGGAGAAGGTGTGCTCGCTGTCTGTGAAGAACAAGGGCTTGCTTGCCCAGTTAAGTGAGGCAAAGCGCAGACAGGCAGAGATTGAATGCAAGCGTCACTTAGCTCGAACTTCTGGAAGATGGAAAGACCCACCCAAGAAAAATGCTGTGAATGAGTTACAGGATGAGCTGATGAGCATTCGCCTTAGAGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATTGAATGCAAGCGTCACTTAGCTC
siRNA sets
AAGTGACGCTTGCATTCAA, CTAAGTGACGCTTGCATTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEVI5_306_ORF_1
1.0942
0.339961496
0.882971021
534
ORF sequence:
ATGAGCATTCGCCTTAGAGAGGCGGAAACACAGGCAGAAATAAGAGAGATGAAGCAAAGGATGATGGAAATGGAGACACAGAACCAGATCAATAGTAACCAACTTCGAAGAGCGGAGCAAGAGGTGAACAGTCTGCAGGAGAAGGTGTGCTCGCTGTCTGTGAAGAACAAGGGCTTGCTTGCCCAGTTAAGTGAGGCAAAGCGCAGACAGGCAGAGATTGAATGCAAGCGTCACTTAGCTCGAACTTCTGGAAGATGGAAAGACCCACCCAAGAAAAATGCTGTGAATGAGTTACAGGATGAGCTGATGAGCATTCGCCTTAGAGAGGCGGAAACACAGGCAGAAATAAGAGAGATGAAGCAAAGGATGATGGAAATGGAGACACAGAACCAGATCAATAGTAACCAACTTCGAAGAGCGGAGCAAGAGGTGAACAGTCTGCAGGAGAAGGTGTGCTCGCTGTCTGTGAAGAACAAGGGCTTGCTTGCCCAGTTAAGTGAGGCAAAGCGCAGACAGGCAGAGATTGAATGCAAG
Peptide sequence:
MSIRLREAETQAEIREMKQRMMEMETQNQINSNQLRRAEQEVNSLQEKVCSLSVKNKGLLAQLSEAKRRQAEIECKRHLARTSGRWKDPPKKNAVNELQDELMSIRLREAETQAEIREMKQRMMEMETQNQINSNQLRRAEQEVNSLQEKVCSLSVKNKGLLAQLSEAKRRQAEIECK
IRESfinder result
IRESfinder score
0.253211118
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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