top of page
mmu_circEif3a_2418
Basic information of circular RNA
PanCircBase ID:
mmu_circEif3a_2418
mm39_Chr. Position ID:
chr19|60757145|60770459|-
Gene name:
Eif3a
Transcript:
ENSMUST00000025955.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
15
Exon/intron indexes:
17,16,15,14,13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,1158,2307,2936,3385,3984,4799,5193,6585,7043,7918,9231,10650,12646,13177
Genomic length:
13314
Exon/intron sizes in nt:
1,85,15,41,23,11,41,05,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr19:60756101-60757145|chr19:60770459-60770605
Splice length:
2418
Other database IDs:
circAtlas: mmu-Eif3a_0032
Human Ortholog IDs:
Mature spliced Sequence:
GTAAACATTAAATCTTTAGAAGATGTTGTTAGGGCATATTTGAAATTAGCAGAGGAAAAAACAGAAGCTGCTAAAGAAGAGTCCCAACAAATGGTGTTAGATATAGAAGATCTGGATAATATTCAGACTCCTGAGAGTGTTCTCTTAAGTGCAGTAAGTGGGGAAGATACTCAGGATCGTACTGACAGATTGCTACTGACTCCCTGGGTCAAGTTCCTGTGGGAATCATACAGGCAGTGTTTGGACCTTCTTCGAAACAATTCTAGAGTAGAGCGCCTTTACCATGATATCGCCCAACAAGCTTTCAAATTCTGCCTCCAGTATACTCGGAAGGCTGAGTTCCGCAAGCTATGTGACAACTTGCGAATGCACTTATCCCAGATTCAGCGCCACCATAACCAAAGCACAGCAATTAATCTTAATAATCCAGAGAGCCAGTCTATGCATTTGGAAACCAGACTTGTTCAGTTGGACAGTGCTATCAGCATGGAATTATGGCAGGAAGCCTTCAAAGCTGTGGAAGATATTCATGGACTATTTTCCTTGTCTAAGAAACCACCTAAGCCTCAGTTGATGGCAAATTACTATAACAAAGTTTCAACAGTGTTTTGGAAATCTGGAAATGCTTTGTTCCATGCATCTACACTTCATCGTCTTTATCATCTGTCTAGAGAAATGAGAAAGAATCTTACACAAGAAGAGATGCAAAGAATGTCTACTAGAGTCCTTTTGGCGACTCTTTCCATTCCTATTACTCCTGAGCGAACTGATATTGCTCGACTTTTGGATATGGATGGTATTATAGTTGAGAAGCAGCGACGCCTTGCCACATTGCTAGGTCTTCAAGCCCCACCCACACGAATCGGCCTAATTAATGATATGGTCAGGTTCAGTGTGCTACAGTATGTTGTCCCAGAAGTGAAAGACCTTTACAACTGGTTGGAGGTGGAATTCAACCCACTAAAACTCTGTGAGAGAGTTACAAAGGTATTAAATTGGGTTAGGGAACAACCTGAAAAAGAACCAGAATTGCAACAATATGTACCACAACTCCAGAACAATACCATACTCCGCCTTCTGCAACAGGTGGCACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACTTCTCTGGTTCCTTTTGTTGATGCTTTCCAACTGGAACGGGCCATAGTAGATGCAGCCAGGCACTGTGACCTGCAGGTACGTATTGACCATACTTCCCGGACTCTGAGTTTTGGATCAGATTTGAATTATGCAACTCGAGAAGATGCCCCAGTTGGCCCTCATCTGCAGAGCATGCCTTCAGAGCAGATAAGAAACCAGCTCACGGCCATGTCCTCAGTGCTTGCCAAAGCACTTGAAGTCATCAGACCTGCTCACATTCTGCAAGAGAAAGAAGAACAGCATCAATTGGCAGTTAATGCATATCTTAAAAATTCAAGAAAAGAGCACCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAAAGAAAAGAGCGTCTTGAGAGTCTGAATATTCAACGTGAGAAGGAAGAACTCGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGAAAGGCTGAAGAAGAAAGGCTGCGCCAAGAGGCAAAGGAGCGAGAGAAGGAACGAATCCTTCAAGAACACGAGCAAATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAGCAGATCAAGAAGACAGAGCTGGGCGCCAAAGCATTTAAAGATATTGACATTGAAGACCTTGAAGAACTGGATCCAGATTTTATTATGGCCAAACAGGTTGAACAACTGGAGAAAGAAAAGAAGGAACTTCAGGAACGCCTGAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGAGCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTATGAGGAACAAAGGGTTAAAGACATGGACCTGTGGGAACAGCAAGAAGAAGAAAGAATCACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAGCATAAGAATAGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGCCTCAAAGCTGCCCGGCAGTCTGTCTACGAGGAAAAACTGAAACAGTTTGAAGAGCGCTTAGCAGAAGAAAGGCATAGTCGCCTAGAAGATCGGAAAAGGCAGCGGAAAGAAGAACGCAAAATAACTTATTACAGAGAAAAGGAAGAAGAAGAGCAGAGGAGGGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGTGCTGAGAGAGCAAAACGGGAGGAAGAACTTAGAGAGTATCAGGAGCGAGTCAAGAAACTAGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGGAAATTGAAGAAAGGGAAAGGCGCAGAGAGGAAGAAAGGAGACTTGGTGATGATCCACTTTCTAGGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGAAAGGGAAAGGCGCAGA
59.89
TGGGACTCTTCTTTAGCAGCT
58.74
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAGAAAGGGAAAGGCGCA
60.18
TGGGACTCTTCTTTAGCAGCT
58.74
150
PCR template sequence:
AGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGGAAATTGAAGAAAGGGAAAGGCGCAGAGAGGAAGAAAGGAGACTTGGTGATGATCCACTTTCTAGGAAGGTAAACATTAAATCTTTAGAAGATGTTGTTAGGGCATATTTGAAATTAGCAGAGGAAAAAACAGAAGCTGCTAAAGAAGAGTCCCAACAAATGGTGTTAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(2), mmu-miR-29b-1-5p(2), mmu-miR-29b-2-5p(2), mmu-miR-9-5p(1), mmu-miR-141-3p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-33-5p(1), mmu-miR-22-3p(1), mmu-miR-382-5p(1), mmu-miR-467b-5p(1), mmu-miR-680(1), mmu-miR-384-3p(1), mmu-miR-697(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
ACTTTCTAGGAAGGTAAACATTAAAT
siRNA sets
ATTTAATGTTTACCTTCCT, TAATGTTTACCTTCCTAGA, TTTAATGTTTACCTTCCTA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF3A_2418_ORF_1
1.1914
0.207571057
1
4746
ORF sequence:
ATGGTGTTAGATATAGAAGATCTGGATAATATTCAGACTCCTGAGAGTGTTCTCTTAAGTGCAGTAAGTGGGGAAGATACTCAGGATCGTACTGACAGATTGCTACTGACTCCCTGGGTCAAGTTCCTGTGGGAATCATACAGGCAGTGTTTGGACCTTCTTCGAAACAATTCTAGAGTAGAGCGCCTTTACCATGATATCGCCCAACAAGCTTTCAAATTCTGCCTCCAGTATACTCGGAAGGCTGAGTTCCGCAAGCTATGTGACAACTTGCGAATGCACTTATCCCAGATTCAGCGCCACCATAACCAAAGCACAGCAATTAATCTTAATAATCCAGAGAGCCAGTCTATGCATTTGGAAACCAGACTTGTTCAGTTGGACAGTGCTATCAGCATGGAATTATGGCAGGAAGCCTTCAAAGCTGTGGAAGATATTCATGGACTATTTTCCTTGTCTAAGAAACCACCTAAGCCTCAGTTGATGGCAAATTACTATAACAAAGTTTCAACAGTGTTTTGGAAATCTGGAAATGCTTTGTTCCATGCATCTACACTTCATCGTCTTTATCATCTGTCTAGAGAAATGAGAAAGAATCTTACACAAGAAGAGATGCAAAGAATGTCTACTAGAGTCCTTTTGGCGACTCTTTCCATTCCTATTACTCCTGAGCGAACTGATATTGCTCGACTTTTGGATATGGATGGTATTATAGTTGAGAAGCAGCGACGCCTTGCCACATTGCTAGGTCTTCAAGCCCCACCCACACGAATCGGCCTAATTAATGATATGGTCAGGTTCAGTGTGCTACAGTATGTTGTCCCAGAAGTGAAAGACCTTTACAACTGGTTGGAGGTGGAATTCAACCCACTAAAACTCTGTGAGAGAGTTACAAAGGTATTAAATTGGGTTAGGGAACAACCTGAAAAAGAACCAGAATTGCAACAATATGTACCACAACTCCAGAACAATACCATACTCCGCCTTCTGCAACAGGTGGCACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACTTCTCTGGTTCCTTTTGTTGATGCTTTCCAACTGGAACGGGCCATAGTAGATGCAGCCAGGCACTGTGACCTGCAGGTACGTATTGACCATACTTCCCGGACTCTGAGTTTTGGATCAGATTTGAATTATGCAACTCGAGAAGATGCCCCAGTTGGCCCTCATCTGCAGAGCATGCCTTCAGAGCAGATAAGAAACCAGCTCACGGCCATGTCCTCAGTGCTTGCCAAAGCACTTGAAGTCATCAGACCTGCTCACATTCTGCAAGAGAAAGAAGAACAGCATCAATTGGCAGTTAATGCATATCTTAAAAATTCAAGAAAAGAGCACCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAAAGAAAAGAGCGTCTTGAGAGTCTGAATATTCAACGTGAGAAGGAAGAACTCGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGAAAGGCTGAAGAAGAAAGGCTGCGCCAAGAGGCAAAGGAGCGAGAGAAGGAACGAATCCTTCAAGAACACGAGCAAATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAGCAGATCAAGAAGACAGAGCTGGGCGCCAAAGCATTTAAAGATATTGACATTGAAGACCTTGAAGAACTGGATCCAGATTTTATTATGGCCAAACAGGTTGAACAACTGGAGAAAGAAAAGAAGGAACTTCAGGAACGCCTGAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGAGCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTATGAGGAACAAAGGGTTAAAGACATGGACCTGTGGGAACAGCAAGAAGAAGAAAGAATCACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAGCATAAGAATAGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGCCTCAAAGCTGCCCGGCAGTCTGTCTACGAGGAAAAACTGAAACAGTTTGAAGAGCGCTTAGCAGAAGAAAGGCATAGTCGCCTAGAAGATCGGAAAAGGCAGCGGAAAGAAGAACGCAAAATAACTTATTACAGAGAAAAGGAAGAAGAAGAGCAGAGGAGGGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGTGCTGAGAGAGCAAAACGGGAGGAAGAACTTAGAGAGTATCAGGAGCGAGTCAAGAAACTAGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGGAAATTGAAGAAAGGGAAAGGCGCAGAGAGGAAGAAAGGAGACTTGGTGATGATCCACTTTCTAGGAAGGTAAACATTAAATCTTTAGAAGATGTTGTTAGGGCATATTTGAAATTAGCAGAGGAAAAAACAGAAGCTGCTAAAGAAGAGTCCCAACAAATGGTGTTAGATATAGAAGATCTGGATAATATTCAGACTCCTGAGAGTGTTCTCTTAAGTGCAGTAAGTGGGGAAGATACTCAGGATCGTACTGACAGATTGCTACTGACTCCCTGGGTCAAGTTCCTGTGGGAATCATACAGGCAGTGTTTGGACCTTCTTCGAAACAATTCTAGAGTAGAGCGCCTTTACCATGATATCGCCCAACAAGCTTTCAAATTCTGCCTCCAGTATACTCGGAAGGCTGAGTTCCGCAAGCTATGTGACAACTTGCGAATGCACTTATCCCAGATTCAGCGCCACCATAACCAAAGCACAGCAATTAATCTTAATAATCCAGAGAGCCAGTCTATGCATTTGGAAACCAGACTTGTTCAGTTGGACAGTGCTATCAGCATGGAATTATGGCAGGAAGCCTTCAAAGCTGTGGAAGATATTCATGGACTATTTTCCTTGTCTAAGAAACCACCTAAGCCTCAGTTGATGGCAAATTACTATAACAAAGTTTCAACAGTGTTTTGGAAATCTGGAAATGCTTTGTTCCATGCATCTACACTTCATCGTCTTTATCATCTGTCTAGAGAAATGAGAAAGAATCTTACACAAGAAGAGATGCAAAGAATGTCTACTAGAGTCCTTTTGGCGACTCTTTCCATTCCTATTACTCCTGAGCGAACTGATATTGCTCGACTTTTGGATATGGATGGTATTATAGTTGAGAAGCAGCGACGCCTTGCCACATTGCTAGGTCTTCAAGCCCCACCCACACGAATCGGCCTAATTAATGATATGGTCAGGTTCAGTGTGCTACAGTATGTTGTCCCAGAAGTGAAAGACCTTTACAACTGGTTGGAGGTGGAATTCAACCCACTAAAACTCTGTGAGAGAGTTACAAAGGTATTAAATTGGGTTAGGGAACAACCTGAAAAAGAACCAGAATTGCAACAATATGTACCACAACTCCAGAACAATACCATACTCCGCCTTCTGCAACAGGTGGCACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACTTCTCTGGTTCCTTTTGTTGATGCTTTCCAACTGGAACGGGCCATAGTAGATGCAGCCAGGCACTGTGACCTGCAGGTACGTATTGACCATACTTCCCGGACTCTGAGTTTTGGATCAGATTTGAATTATGCAACTCGAGAAGATGCCCCAGTTGGCCCTCATCTGCAGAGCATGCCTTCAGAGCAGATAAGAAACCAGCTCACGGCCATGTCCTCAGTGCTTGCCAAAGCACTTGAAGTCATCAGACCTGCTCACATTCTGCAAGAGAAAGAAGAACAGCATCAATTGGCAGTTAATGCATATCTTAAAAATTCAAGAAAAGAGCACCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAAAGAAAAGAGCGTCTTGAGAGTCTGAATATTCAACGTGAGAAGGAAGAACTCGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGAAAGGCTGAAGAAGAAAGGCTGCGCCAAGAGGCAAAGGAGCGAGAGAAGGAACGAATCCTTCAAGAACACGAGCAAATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAGCAGATCAAGAAGACAGAGCTGGGCGCCAAAGCATTTAAAGATATTGACATTGAAGACCTTGAAGAACTGGATCCAGATTTTATTATGGCCAAACAGGTTGAACAACTGGAGAAAGAAAAGAAGGAACTTCAGGAACGCCTGAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGAGCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTATGAGGAACAAAGGGTTAAAGACATGGACCTGTGGGAACAGCAAGAAGAAGAAAGAATCACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAGCATAAGAATAGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGCCTCAAAGCTGCCCGGCAGTCTGTCTACGAGGAAAAACTGAAACAGTTTGAAGAGCGCTTAGCAGAAGAAAGGCATAGTCGCCTAGAAGATCGGAAAAGGCAGCGGAAAGAAGAACGCAAAATAACTTATTACAGAGAAAAGGAAGAAGAAGAGCAGAGGAGGGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGTGCTGAGAGAGCAAAACGGGAGGAAGAACTTAGAGAGTATCAGGAGCGAGTCAAGAAACTAGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGGAAATTGAAGAAAGGGAAAGGCGCAGAGAGGAAGAAAGGAGACTTGGTGATGATCCACTTTCTAGGAAG
Peptide sequence:
MVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQEEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFSVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVAQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPVGPHLQSMPSEQIRNQLTAMSSVLAKALEVIRPAHILQEKEEQHQLAVNAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRVKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHSRLEDRKRQRKEERKITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDDPLSRKVNIKSLEDVVRAYLKLAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQEEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFSVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVAQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPVGPHLQSMPSEQIRNQLTAMSSVLAKALEVIRPAHILQEKEEQHQLAVNAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRVKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHSRLEDRKRQRKEERKITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDDPLSRK
IRESfinder result
IRESfinder score
0.530885553
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page