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mmu_circEhbp1_1110
Basic information of circular RNA
PanCircBase ID:
mmu_circEhbp1_1110
mm39_Chr. Position ID:
chr11|22045317|22051230|-
Gene name:
Ehbp1
Transcript:
ENSMUST00000045167.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
13,12,11
Exon/intron offsets:
0,5104,5734
Genomic length:
5913
Exon/intron sizes in nt:
8,82,49,179
Flanking exon/introns:
chr11:22039668-22045317|chr11:22051230-22087837
Splice length:
1110
Other database IDs:
CIRCpedia: MMU_CIRCpedia_13452, circAtlas: mmu-Ehbp1_0042
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_92056
Human Ortholog IDs:
Mature spliced Sequence:
CCAAGCCCGATACCAAGTCCTGTTCTGGGGCAAAAGCCAAACGCTAGTCAGTCGCTGCTTGCATGGTGCAGAGAAGTTACAAAAAACTACCGGGGCGTGAAAATCACCAACTTTACCACATCGTGGAGGAATGGCTTATCATTCTGTGCAATTTTACACCACTTTAGACCAGACTTAATCGACTACAAGTCTCTGAATCCTCAAGATATTAAAGAAAACAACAAAAAGGCTTACGATGGATTTGCCAGCATTGGAATTTCCCGTTTACTGGAACCTTCTGATATGGTTTTATTAGCAATCCCTGACAAACTGACTGTTATGACTTATCTCTATCAAATAAGGGCACATTTTAGCGGACAAGAACTAAATGTGGTTCAGATAGAGGAAAACAGCAGTAAGAGCACATATAAAGTTGGAAATTATGAGACAGACACAAACAGTTCTGTGGATCAAGAAAAGTTCTACGCTGAACTCAGCGATCTGAAGCGAGAGCCTGAACCTCACCAGCCTGCCCGTGGGGCTGTGGATCTGCTGTCGCAGGATGACTCTGTGTTTGTAACTGACAGTGGGGTGGGAGAGTCTGAAAGTGAACACCAGACTCCAGATGACCACCTTAGTCCAAGCACAGCCTCCCCTTACTACCGCAGGACTAAGAGTGACACGGAGCCCCAAAAGTCGCAGCAGAGCTCCGCGAGGACTTCAGGGTCTGATGACCCTGGGTTAAGTTCTAGTACTGATTCAGCTCAAGCCCTGGCTTCCTTAGGCAAGAAGAGACTAAAAGCTGAGAACTTGGAACTAAGTGACTTATGTGTCAGTGACAAGAAGAAGGATGTGTCTCCACTCTCAGCTTACGAGCAAAAGCTTCAGACTGTACATGCCAGCAGTGATATGGAACAAGGGAAAATGGAGAAGTCCAGATCTTTAGAATGCAGATTGGATGGTGAATTGGCTATCACAAAGCCAAATGTATCCTCTCCTTCTAAACTTGGATATAACAGAGACACAGACTTCACGAAAAAACCATGTGCTTCTCTGAGGCAGATAGAGTCTGATCCAGATGCAGACAAGAGCACTTTAAATCATGCAGACCACCCCAACAAAGCAGTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCATGTGCTTCTCTGAGGC
60.04
AGCGACTGACTAGCGTTTGG
60.39
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCATGTGCTTCTCTGAGGCA
60.04
AGCGACTGACTAGCGTTTGG
60.39
146
PCR template sequence:
CACGAAAAAACCATGTGCTTCTCTGAGGCAGATAGAGTCTGATCCAGATGCAGACAAGAGCACTTTAAATCATGCAGACCACCCCAACAAAGCAGTTCAGCCAAGCCCGATACCAAGTCCTGTTCTGGGGCAAAAGCCAAACGCTAGTCAGTCGCTGCTTGCATGGTGCAGAGAAGTTACAAAAAACTACCGGGGCGTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-223-3p(2), mmu-miR-20b-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-32-5p(1), mmu-miR-217-5p(1), mmu-miR-138-5p(1), mmu-miR-136-5p(1), mmu-miR-363-3p(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-383-5p(1), mmu-miR-224-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAAGCAGTTCAGCCAAGCCCGATAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEHBP1_1110_ORF_1
1.0268
0.174207319
0.999987775
1938
ORF sequence:
ATGGTTTTATTAGCAATCCCTGACAAACTGACTGTTATGACTTATCTCTATCAAATAAGGGCACATTTTAGCGGACAAGAACTAAATGTGGTTCAGATAGAGGAAAACAGCAGTAAGAGCACATATAAAGTTGGAAATTATGAGACAGACACAAACAGTTCTGTGGATCAAGAAAAGTTCTACGCTGAACTCAGCGATCTGAAGCGAGAGCCTGAACCTCACCAGCCTGCCCGTGGGGCTGTGGATCTGCTGTCGCAGGATGACTCTGTGTTTGTAACTGACAGTGGGGTGGGAGAGTCTGAAAGTGAACACCAGACTCCAGATGACCACCTTAGTCCAAGCACAGCCTCCCCTTACTACCGCAGGACTAAGAGTGACACGGAGCCCCAAAAGTCGCAGCAGAGCTCCGCGAGGACTTCAGGGTCTGATGACCCTGGGTTAAGTTCTAGTACTGATTCAGCTCAAGCCCTGGCTTCCTTAGGCAAGAAGAGACTAAAAGCTGAGAACTTGGAACTAAGTGACTTATGTGTCAGTGACAAGAAGAAGGATGTGTCTCCACTCTCAGCTTACGAGCAAAAGCTTCAGACTGTACATGCCAGCAGTGATATGGAACAAGGGAAAATGGAGAAGTCCAGATCTTTAGAATGCAGATTGGATGGTGAATTGGCTATCACAAAGCCAAATGTATCCTCTCCTTCTAAACTTGGATATAACAGAGACACAGACTTCACGAAAAAACCATGTGCTTCTCTGAGGCAGATAGAGTCTGATCCAGATGCAGACAAGAGCACTTTAAATCATGCAGACCACCCCAACAAAGCAGTTCAGCCAAGCCCGATACCAAGTCCTGTTCTGGGGCAAAAGCCAAACGCTAGTCAGTCGCTGCTTGCATGGTGCAGAGAAGTTACAAAAAACTACCGGGGCGTGAAAATCACCAACTTTACCACATCGTGGAGGAATGGCTTATCATTCTGTGCAATTTTACACCACTTTAGACCAGACTTAATCGACTACAAGTCTCTGAATCCTCAAGATATTAAAGAAAACAACAAAAAGGCTTACGATGGATTTGCCAGCATTGGAATTTCCCGTTTACTGGAACCTTCTGATATGGTTTTATTAGCAATCCCTGACAAACTGACTGTTATGACTTATCTCTATCAAATAAGGGCACATTTTAGCGGACAAGAACTAAATGTGGTTCAGATAGAGGAAAACAGCAGTAAGAGCACATATAAAGTTGGAAATTATGAGACAGACACAAACAGTTCTGTGGATCAAGAAAAGTTCTACGCTGAACTCAGCGATCTGAAGCGAGAGCCTGAACCTCACCAGCCTGCCCGTGGGGCTGTGGATCTGCTGTCGCAGGATGACTCTGTGTTTGTAACTGACAGTGGGGTGGGAGAGTCTGAAAGTGAACACCAGACTCCAGATGACCACCTTAGTCCAAGCACAGCCTCCCCTTACTACCGCAGGACTAAGAGTGACACGGAGCCCCAAAAGTCGCAGCAGAGCTCCGCGAGGACTTCAGGGTCTGATGACCCTGGGTTAAGTTCTAGTACTGATTCAGCTCAAGCCCTGGCTTCCTTAGGCAAGAAGAGACTAAAAGCTGAGAACTTGGAACTAAGTGACTTATGTGTCAGTGACAAGAAGAAGGATGTGTCTCCACTCTCAGCTTACGAGCAAAAGCTTCAGACTGTACATGCCAGCAGTGATATGGAACAAGGGAAAATGGAGAAGTCCAGATCTTTAGAATGCAGATTGGATGGTGAATTGGCTATCACAAAGCCAAATGTATCCTCTCCTTCTAAACTTGGATATAACAGAGACACAGACTTCACGAAAAAACCATGTGCTTCTCTGAGGCAGATAGAGTCTGATCCAGATGCAGACAAGAGCACTTTAAATCATGCAGACCACCCCAACAAAGCAGTTCAG
Peptide sequence:
MVLLAIPDKLTVMTYLYQIRAHFSGQELNVVQIEENSSKSTYKVGNYETDTNSSVDQEKFYAELSDLKREPEPHQPARGAVDLLSQDDSVFVTDSGVGESESEHQTPDDHLSPSTASPYYRRTKSDTEPQKSQQSSARTSGSDDPGLSSSTDSAQALASLGKKRLKAENLELSDLCVSDKKKDVSPLSAYEQKLQTVHASSDMEQGKMEKSRSLECRLDGELAITKPNVSSPSKLGYNRDTDFTKKPCASLRQIESDPDADKSTLNHADHPNKAVQPSPIPSPVLGQKPNASQSLLAWCREVTKNYRGVKITNFTTSWRNGLSFCAILHHFRPDLIDYKSLNPQDIKENNKKAYDGFASIGISRLLEPSDMVLLAIPDKLTVMTYLYQIRAHFSGQELNVVQIEENSSKSTYKVGNYETDTNSSVDQEKFYAELSDLKREPEPHQPARGAVDLLSQDDSVFVTDSGVGESESEHQTPDDHLSPSTASPYYRRTKSDTEPQKSQQSSARTSGSDDPGLSSSTDSAQALASLGKKRLKAENLELSDLCVSDKKKDVSPLSAYEQKLQTVHASSDMEQGKMEKSRSLECRLDGELAITKPNVSSPSKLGYNRDTDFTKKPCASLRQIESDPDADKSTLNHADHPNKAVQ
IRESfinder result
IRESfinder score
0.603615574
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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