top of page
mmu_circDock8_1046
Basic information of circular RNA
PanCircBase ID:
mmu_circDock8_1046
mm39_Chr. Position ID:
chr19|25140304|25152824|+
Gene name:
Dock8
Transcript:
ENSMUST00000025831.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
31,32,33,34,35,36,37,38
Exon/intron offsets:
0,507,1380,6462,8560,9516,11233,12419
Genomic length:
12520
Exon/intron sizes in nt:
1,83,13,08,89,71,35,15,00,00,000
Flanking exon/introns:
chr19:25138519-25140304|chr19:25152824-25154191
Splice length:
1046
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CCCTATAAGCAGTACAACATGCTGAATGCAGACACCACCCGCCACCTCATGATTTGCTTCCTGTGGATCATGAAAAATGCTGATCAGAGCCTCATCAGGAAGTGGATCGCTGACCTGCCTTCCATGCAGCTCAATAGGATTCTGGACCTGCTGTTCATCTGTGTCTCCTGCTTTGAATACAAGGGAAAGCAGAGTTCTGACAAAGTCAGTAACCAGGTCCTGCAGAAGTCAAGAGACGTCAAGGCCAAGCTGGAAGAGGCCCTGCTCCGTGGGGAAGGAGCCCGTGGGGAGATGATGCGCCGTCGCATTCCAGGGACTGACCGGTTTCCAGGCATAAATGAAAATCTGAGATGGAGGAAAGAGCAGACACAGTGGCGGCAGGCTAATGAGAAGCTGGACAAAACAAAGGCAGAGTTAGATCAAGAAGCCTTGATCAGTGGCAACCTGGCTACAGAAGCTAATTTGATCATCCTGGATATGCAGGAGAACATCATCCAGGCAAGCTCCGCCCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTCCTCCGGGTCCTGGTGAATTCTCTGAGCTGTGACCAGAGCACCACCTACCTGACTCACTGTTTCGCAACCCTCCGAGCCCTCATCGCCAAGTTTGGAGACCTGCTGTTCGAGGAGGAGATGGAGCAGTGTGCTGACCTGTGTCAGCGGGTGCTACATCACTGCAGCAGCAGCATGGACGTCACACGGAGCCAAGCCTGCGCCACCCTCTACCTCCTCATGCGGTTCAGCTTCGGAGCCACCAGTAACTTTGCAAGGGTAAAGATGCAAGTGACCATGGCACTGGCATCCCTGGTAGGCAAAGCACCAGACTTCAACGAAGAGCACCTGAGAAGGTCCTTAAGGACAATTTTGGCCTATTCAGAAGAGGACACGGCCATGCAGACAACTCCTTTTCCCATGCAGGTGGAGGAACTTCTCTGCAATCTGAACAGCATTCTGTACGACACAGTGAAGATGAGGGAATTCCAGGAAGACCCTGAGATGCTTATGGACCTCATGTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGGAATTCCAGGAAGACCC
59.79
CCTGATGAGGCTCTGATCAGC
60.27
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACGACACAGTGAAGATGAGGG
59.73
CCTGATGAGGCTCTGATCAGC
60.27
163
PCR template sequence:
TGGAGGAACTTCTCTGCAATCTGAACAGCATTCTGTACGACACAGTGAAGATGAGGGAATTCCAGGAAGACCCTGAGATGCTTATGGACCTCATGTACAGCCCTATAAGCAGTACAACATGCTGAATGCAGACACCACCCGCCACCTCATGATTTGCTTCCTGTGGATCATGAAAAATGCTGATCAGAGCCTCATCAGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-335-3p(2), mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-214-3p(1), mmu-let-7d-5p(1), mmu-miR-224-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACCTCATGTACAGCCCTATAAGCAGT
siRNA sets
TATAGGGCTGTACATGAGG, TTATAGGGCTGTACATGAG, CTTATAGGGCTGTACATGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDOCK8_1046_ORF_1
1.2205
0.382646758
0.997851791
1053
ORF sequence:
ATGCTGAATGCAGACACCACCCGCCACCTCATGATTTGCTTCCTGTGGATCATGAAAAATGCTGATCAGAGCCTCATCAGGAAGTGGATCGCTGACCTGCCTTCCATGCAGCTCAATAGGATTCTGGACCTGCTGTTCATCTGTGTCTCCTGCTTTGAATACAAGGGAAAGCAGAGTTCTGACAAAGTCAGTAACCAGGTCCTGCAGAAGTCAAGAGACGTCAAGGCCAAGCTGGAAGAGGCCCTGCTCCGTGGGGAAGGAGCCCGTGGGGAGATGATGCGCCGTCGCATTCCAGGGACTGACCGGTTTCCAGGCATAAATGAAAATCTGAGATGGAGGAAAGAGCAGACACAGTGGCGGCAGGCTAATGAGAAGCTGGACAAAACAAAGGCAGAGTTAGATCAAGAAGCCTTGATCAGTGGCAACCTGGCTACAGAAGCTAATTTGATCATCCTGGATATGCAGGAGAACATCATCCAGGCAAGCTCCGCCCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTCCTCCGGGTCCTGGTGAATTCTCTGAGCTGTGACCAGAGCACCACCTACCTGACTCACTGTTTCGCAACCCTCCGAGCCCTCATCGCCAAGTTTGGAGACCTGCTGTTCGAGGAGGAGATGGAGCAGTGTGCTGACCTGTGTCAGCGGGTGCTACATCACTGCAGCAGCAGCATGGACGTCACACGGAGCCAAGCCTGCGCCACCCTCTACCTCCTCATGCGGTTCAGCTTCGGAGCCACCAGTAACTTTGCAAGGGTAAAGATGCAAGTGACCATGGCACTGGCATCCCTGGTAGGCAAAGCACCAGACTTCAACGAAGAGCACCTGAGAAGGTCCTTAAGGACAATTTTGGCCTATTCAGAAGAGGACACGGCCATGCAGACAACTCCTTTTCCCATGCAGGTGGAGGAACTTCTCTGCAATCTGAACAGCATTCTGTACGACACAGTGAAGATGAGGGAATTCCAGGAAGACCCTGAGATGCTTATGGACCTCATGTACAGCCCTATAAGCAGTACAACATGCTGA
Peptide sequence:
MLNADTTRHLMICFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGINENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRRSLRTILAYSEEDTAMQTTPFPMQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYSPISSTTC
IRESfinder result
IRESfinder score
0.414892441
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page