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mmu_circDiaph3_2824
Basic information of circular RNA
PanCircBase ID:
mmu_circDiaph3_2824
mm39_Chr. Position ID:
chr14|87009521|87275022|-
Gene name:
Diaph3
Transcript:
ENSMUST00000022599.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
23
Exon/intron indexes:
27,26,25,24,23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5
Exon/intron offsets:
0,521,38250,56906,62889,79986,94317,133851,137943,146683,193356,194239,197363,206528,209485,212696,213534,213929,227923,230716,235089,235240,265370
Genomic length:
265501
Exon/intron sizes in nt:
60,96,136,163,127,130,240,102,95,96,115,309,105,65,119,117,119,111,106,137,70,75,131
Flanking exon/introns:
chr14:86894018-87009521|chr14:87275022-87310717
Splice length:
2824
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGAAGTCTTAGAAGTAGCCGACAGATCTCACCTCAGGAATTTCTCCATGAGCTGAAAATGGGTTACACAGACGAGAGACTTTTCACGTATCTGGAGTCACTCCGAGTATCATTGACCAGTCATCCTGTGAGTTGGGTGCAAAGCTTTGGACACGAGGGACTCGGATTATTGCTGGACATTTTGGAAAAACTAATTAATGGGCAAATCCAAGAAAAAGTTGTGAAGAAGACTCAGCACAAAGTCATCCAGTGTCTGAGAGCCCTGATGAACACACAGTATGGCTTAGAAAGGATTATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGATCCCAGGCAGCCCGCTATGATGGCAGACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAAAGCATCCTTGAAGAAGTGTTAGAAGCCTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTTCCATTGTGGAAGGCCTCCGGCATAACTCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCTCGTTACATCTCCTGATGATTTGGACTTCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAACTTAAAGGGCATTAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGCACAAAGAAGAAGATTTGAGTGAGTTTTTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATCTGATGTTTACAGCATGTTATGGGACACAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTTCAGCATCTCCTGCTCATTCGCAATGATCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTGTGTCACAGATTGTATTACATAGAGATGGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGACTTAAGTCAGTTTGTAGATGTTTGCATAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAACATTGCAAGAAATTTGAAAAAGAGTGTACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGGCAAAGATTAATGAGCTTCAAGCAGAGTTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTACAAAAATTCCTTTGCAACCTTCAGTAGAAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCACTCAGTGGAGGAGTGCCGCCTCCCCCACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAATGCCATTTGGTGGCCCTGTACCACCACCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATTAAACCTGCCATTTGGTTTGAAACCAAAGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCGGACCAAATGAAATGTCTGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAAATAGGGATTTGCTTTGTAAACTTGAGAACACTTTTTGTTGCCAAGAAAAAGAGAAAAGGAATACAAATGACTTTGATGAGAAGAAAGTTATTAAGAAGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAGAACCTTTCAATCTTCCTGAGCTCCTTCCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGGATGAAACACAGTTGTCAGAGTCCATGATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAAGTCATTGTCCCAGTTTAGAAGTGACTATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGCAATGTGAAGAGACTCCGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAAGTTGCTGGAACTTGTGTTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTGACCTTAGCTCTCTCTGTAAACTGAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATTTCCTGGTAGATGTATGTGAAGAAAAGCATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGACAAAGCTAGCAGAGTCTCTGTAGAAATGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGAATTTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCATCTTCGTTATCAGTGCGAACGAGCAGTATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTACCAGAGTATAATGGGCTACTATGCTGTCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTGAACAACTTCAGAACTTCATTTATGCTAGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGGAGAAACGTGCCAGGATAGCGAAAGAGCGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCGCTTACTAGAAATGAAAACTGAGGGAGATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCAGGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGATGTCTCAGAGGCCTGTTCTCAAAGTTTGTAACCATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGAGTCTCAGCCCGATGTC
59.9
ACGTGAAAAGTCTCTCGTCTGT
59.64
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTTCCGCGACAGAAGAAA
60.95
GTCTCTCGTCTGTGTAACCCA
59.12
179
PCR template sequence:
GCCTTCCGCGACAGAAGAAAAAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGATGTCTCAGAGGCCTGTTCTCAAAGTTTGTAACCATGGGAAGTCTTAGAAGTAGCCGACAGATCTCACCTCAGGAATTTCTCCATGAGCTGAAAATGGGTTACACAGACGAGAGACTTTTCACGTATCTGGAGTCAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
30
Interacting miRNAs (#binding sites)
mmu-miR-335-3p(3), mmu-miR-30c-5p(2), mmu-miR-127-5p(2), mmu-miR-338-3p(2), mmu-miR-9-5p(1), mmu-miR-23b-3p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-135a-5p(1), mmu-miR-30e-5p(1), mmu-miR-200c-3p(1), mmu-miR-205-5p(1), mmu-miR-30a-5p(1), mmu-miR-19a-3p(1), mmu-miR-18a-5p(1), mmu-miR-23a-3p(1), mmu-miR-30d-5p(1), mmu-miR-30b-5p(1), mmu-miR-676-3p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-382-5p(1), mmu-miR-183-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-342-3p(1), mmu-miR-377-3p(1), mmu-miR-539-5p(1), mmu-miR-3087-3p(1), mmu-miR-1904(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTTGTAACCATGGGAAGTCTTAGAA
siRNA sets
TTCTAAGACTTCCCATGGT, AAGACTTCCCATGGTTACA, TAAGACTTCCCATGGTTAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDIAPH3_2824_ORF_1
1.1229
0.156604567
0.99999998
2826
ORF sequence:
ATGGGAAGTCTTAGAAGTAGCCGACAGATCTCACCTCAGGAATTTCTCCATGAGCTGAAAATGGGTTACACAGACGAGAGACTTTTCACGTATCTGGAGTCACTCCGAGTATCATTGACCAGTCATCCTGTGAGTTGGGTGCAAAGCTTTGGACACGAGGGACTCGGATTATTGCTGGACATTTTGGAAAAACTAATTAATGGGCAAATCCAAGAAAAAGTTGTGAAGAAGACTCAGCACAAAGTCATCCAGTGTCTGAGAGCCCTGATGAACACACAGTATGGCTTAGAAAGGATTATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGATCCCAGGCAGCCCGCTATGATGGCAGACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAAAGCATCCTTGAAGAAGTGTTAGAAGCCTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTTCCATTGTGGAAGGCCTCCGGCATAACTCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCTCGTTACATCTCCTGATGATTTGGACTTCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAACTTAAAGGGCATTAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGCACAAAGAAGAAGATTTGAGTGAGTTTTTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATCTGATGTTTACAGCATGTTATGGGACACAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTTCAGCATCTCCTGCTCATTCGCAATGATCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTGTGTCACAGATTGTATTACATAGAGATGGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGACTTAAGTCAGTTTGTAGATGTTTGCATAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAACATTGCAAGAAATTTGAAAAAGAGTGTACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGGCAAAGATTAATGAGCTTCAAGCAGAGTTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTACAAAAATTCCTTTGCAACCTTCAGTAGAAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCACTCAGTGGAGGAGTGCCGCCTCCCCCACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAATGCCATTTGGTGGCCCTGTACCACCACCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATTAAACCTGCCATTTGGTTTGAAACCAAAGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCGGACCAAATGAAATGTCTGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAAATAGGGATTTGCTTTGTAAACTTGAGAACACTTTTTGTTGCCAAGAAAAAGAGAAAAGGAATACAAATGACTTTGATGAGAAGAAAGTTATTAAGAAGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAGAACCTTTCAATCTTCCTGAGCTCCTTCCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGGATGAAACACAGTTGTCAGAGTCCATGATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAAGTCATTGTCCCAGTTTAGAAGTGACTATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGCAATGTGAAGAGACTCCGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAAGTTGCTGGAACTTGTGTTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTGACCTTAGCTCTCTCTGTAAACTGAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATTTCCTGGTAGATGTATGTGAAGAAAAGCATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGACAAAGCTAGCAGAGTCTCTGTAGAAATGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGAATTTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCATCTTCGTTATCAGTGCGAACGAGCAGTATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTACCAGAGTATAATGGGCTACTATGCTGTCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTGAACAACTTCAGAACTTCATTTATGCTAGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGGAGAAACGTGCCAGGATAGCGAAAGAGCGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCGCTTACTAGAAATGAAAACTGAGGGAGATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCAGGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGATGTCTCAGAGGCCTGTTCTCAAAGTTTGTAACCAT
Peptide sequence:
MGSLRSSRQISPQEFLHELKMGYTDERLFTYLESLRVSLTSHPVSWVQSFGHEGLGLLLDILEKLINGQIQEKVVKKTQHKVIQCLRALMNTQYGLERIMSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHNSVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEFFHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRDGTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAELQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPPPPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLENTFCCQEKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESMIQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEKHADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSIFVISANEQYEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKERAEKERLERQQEKKRLLEMKTEGDETGVMDSLLEALQSGAAFRDRRKRTPKLKDIRQSLSPMSQRPVLKVCNH
IRESfinder result
IRESfinder score
0.584544996
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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